Open Access

ARTICLE

Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5

Mengyi Huang, Xin Gong

Department of Neurosurgery, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha City, P.R. China

Oncology Research 2018, 26(7), 1103-1111. https://doi.org/10.3727/096504018X15164123839400

Abstract

As a member of the miRNA family, let-7c has been identified as a tumor suppressor in many cancers. However, the molecular biological function of let-7c in glioma has not been elucidated. The aim of this study was to explore let-7c expression levels and evaluate its function in glioma cells. We first measured the expression of let-7c in four glioma cell lines and a normal cell line by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), and the results showed that let-7c was downregulated in glioma cells. By applying gain-of-function and loss-of-function assays, the experiments suggested that dysregulation of let-7c could obviously affect cell proliferation, metastasis, and invasion. Based on online bioinformatics analysis and Dual-Luciferase Reporter assays, we found that E2F5 was a target gene of let-7c and contributed to the function of let-7c in glioma cells. Our investigations indicated that loss of let-7c contributed to the progression of glioma cells.

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Keywords

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