Open Access
ARTICLE
Long Noncoding RNA ATB Promotes Proliferation, Migration, and Invasion in Bladder Cancer by Suppressing MicroRNA-126
Xingquan Zhai, Wei Xu
Department of Urology, Zoucheng People’s Hospital, Zoucheng, Shandong, P.R. China
Oncology Research 2018, 26(7), 1063-1072. https://doi.org/10.3727/096504018X15152072098476
Abstract
This study aimed to explore the biological functions of long noncoding RNA activated by transforming growth
factor-b (lncRNA-ATB) in bladder cancer cells. For the expressions of lncRNA-ATB, miR-126, and KRAS,
T24 cells were transfected with their specific vectors/shRNA or mimic/inhibitor. Then cell viability, migration,
invasion, and apoptosis as well as the protein levels of apoptosis-related factors and PI3K/AKT and mTOR
signal pathways were measured. The relationships of lncRNA-ATB and miR-126 or miR-126 and KRAS were
analyzed by Dual-Luciferase Reporter assay. Functional experiments showed that lncRNA-ATB overexpression significantly promoted cell viability, migration, and invasion in T24 cells. lncRNA-ATB was a molecular
sponge of miR-126 and exerted tumor-promoting effects by downregulation of miR-126. Moreover, KRAS
was a direct target of miR-126 and was negatively regulated by miR-126. Finally, overexpression of KRAS
increased cell viability, migration, and invasion, as well as activated PI3K/AKT and mTOR signaling pathways
in T24 cells. The results revealed that lncRNA-ATB was an oncogene, which promoted cell proliferation,
migration, and invasion by regulating miR-126 in bladder cancer. These findings may provide a potential prognostic biomarker and a therapeutic target for bladder cancer.
Keywords
Cite This Article
APA Style
Zhai, X., Xu, W. (2018). Long noncoding RNA ATB promotes proliferation, migration, and invasion in bladder cancer by suppressing microrna-126. Oncology Research, 26(7), 1063-1072. https://doi.org/10.3727/096504018X15152072098476
Vancouver Style
Zhai X, Xu W. Long noncoding RNA ATB promotes proliferation, migration, and invasion in bladder cancer by suppressing microrna-126. Oncol Res. 2018;26(7):1063-1072 https://doi.org/10.3727/096504018X15152072098476
IEEE Style
X. Zhai and W. Xu, "Long Noncoding RNA ATB Promotes Proliferation, Migration, and Invasion in Bladder Cancer by Suppressing MicroRNA-126," Oncol. Res., vol. 26, no. 7, pp. 1063-1072. 2018. https://doi.org/10.3727/096504018X15152072098476