Open Access

ARTICLE

MicroRNA-379 Suppresses Cervical Cancer Cell Proliferation and Invasion by Directly Targeting V-crk Avian Sarcoma Virus CT10 Oncogene Homolog-Like (CRKL)

Xi Shi^*1, Xiao Xiao^†1, Na Yuan^*, Shili Zhang^*, Fukang Yuan^‡, Xiaohong Wang^§

* Institute of Audiology and Balance Science, Xuzhou Medical University, Xuzhou, P.R. China
† Reproductive Center, Wuxi Maternal and Child Health-Care Hospital, Wuxi, P.R. China
‡ Department of Vascular Surgery, XuZhou Central Hospital, Xuzhou, P.R. China
§ Department of Obstetrics and Gynecology, Chinese PLA 101 Hospital, Wuxi, P.R. China

Oncology Research 2018, 26(7), 987-996. https://doi.org/10.3727/096504017X15140534417184

Abstract

Cervical cancer is the fourth most common malignancy among females worldwide. MicroRNA-379 (miR-379) is aberrantly expressed in multiple human cancer types. However, the expression pattern, roles, and detailed regulatory mechanisms of miR-379 in cervical cancer remain unknown. In this study, we found that miR-379 expression was downregulated in cervical cancer tissues and cell lines. Low miR-379 expression was correlated with International Federation of Gynecology and Obstetrics (FIGO) stage, lymph node metastasis, and distant metastasis. Additionally, miR-379 overexpression suppressed the proliferation and invasion of cervical cancer cells. Furthermore, V-crk avian sarcoma virus CT10 oncogene homolog-like (CRKL) was identified as a direct target of miR-379 in cervical cancer. CRKL was upregulated in cancer tissues and negatively correlated with miR-379 expression. Moreover, restored CRKL expression rescued the inhibitory effects of miR-379 overexpression on cell proliferation and invasion. In conclusion, miR-379 may serve as a tumor suppressor in cervical cancer by directly targeting CRKL. Restoring miR-379 expression may be an effective strategy for the treatment of cervical cancer.

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