Open Access

ARTICLE

Lulin Zhang, Jun Yao, Wenyao Li, Ce Zhang
Oncology Research, Vol.26, No.6, pp. 827-836, 2018, DOI:10.3727/096504017X14934840662335
Abstract Pancreatic ductal adenocarcinoma (PDAC) is one of the leading causes of cancer deaths due to its highly aggressive biological nature and resistance to chemotherapy. Previous studies indicate that miR-21 is an important regulator in the activation of cancer-associated fibroblasts (CAFs). However, whether miR-21 in CAFs would regulate PDAC’s tumor microenvironment and lead to drug resistance remain unknown. In this study, we evaluated the relationship between CAF activation, miR-21 expression, and drug resistance using tumor samples from PDAC patients. We changed the miR-21 expression level in CAFs and tested its roles in regulating the function of… More >

View

61

Download

56

Open Access

ARTICLE

Gong-Yi Lv, Jun Miao, Xiao-Lin Zhang
Oncology Research, Vol.26, No.6, pp. 837-846, 2018, DOI:10.3727/096504017X14920318811721
Abstract Abnormal expression of long noncoding RNAs (lncRNAs) often contributes to the unrestricted growth and invasion of cancer cells. lncRNA X-inactive specific transcript (XIST) expression is upregulated in several cancers; however, its underlying mechanism in osteosarcoma (OS) has not been elucidated. In the present study, we found that XIST expression was significantly increased in OS tissues and cell lines by LncRNA Profiler and qRT-PCR. The effects of XIST and miR-320b on OS cell proliferation and invasion were studied by MTT and Transwell invasion assays. The competing relationship between XIST and miR-320b was confirmed by luciferase reporter More >

View

60

Download

62

Open Access

ARTICLE

Jifu Song, Zhibin Guan, Maojiang Li, Sha Sha, Chao Song, Zhiwei Gao, Yongli Zhao
Oncology Research, Vol.26, No.6, pp. 847-856, 2018, DOI:10.3727/096504017X15016337254632
Abstract MicroRNAs (miRNAs) have emerged as pivotal regulators of the development and progression of gastric cancer. Studies have shown that miR-154 is a novel cancer-associated miRNA involved in various cancers. However, the role of miR-154 in gastric cancer remains unknown. Here we aimed to investigate the biological function and the potential molecular mechanism of miR-154 in gastric cancer. We found that miR-154 was significantly downregulated in gastric cancer tissues and cell lines. The overexpression of miR-154 significantly repressed the growth and invasion of gastric cancer cells. Bioinformatics analysis and Dual-Luciferase Reporter Assay data showed that miR-154… More >

View

54

Download

51

Open Access

ARTICLE WITHDRAWN

Zhu Zhankun¹, Dai Jinhua¹, Liao Yufeng¹, Ma Jianbo¹, Zhou Wei²
Oncology Research, Vol.26, No.6, pp. 857-867, 2018, DOI:10.3727/096504017X15016337254597
Abstract THIS ARTICLE WAS WITHDRAWN BY THE PUBLISHERS IN OCTOBER 2020. More >

View

47

Download

43

Open Access

ARTICLE

Xianbiao Shi, Xiaoqiao Tang, Lei Su
Oncology Research, Vol.26, No.6, pp. 869-878, 2018, DOI:10.3727/096504017X15123838050075
Abstract This study aimed to investigate the effect of long noncoding RNA PTENP1 in the development of breast cancer (BC). Quantitative real-time PCR was utilized to determine the expression of PTENP1 in tissues and cell lines. pcDNA3.1 and shRNA were used to over- and low-express PTENP1 in BC cell lines, and miR-19b mimic and inhibitor were utilized to over- and low-express miR-19b. Then the abilities of cell survival, apoptosis, migration, and invasion were assessed in BC cells with different expression levels of PTENP1 and miR-19b. The expression of PTENP1 was significantly downregulated in both BC tissues More >

View

57

Download

48

Open Access

ARTICLE

Cheng Zhang^*, Jing-Yi Li^*, Fu-Zhou Tian^†, Gang Zhao^‡, Hai Hu^‡, Yue-Feng Ma^*, Yu-Long Yang^*
Oncology Research, Vol.26, No.6, pp. 879-888, 2018, DOI:10.3727/096504017X15024935181289
Abstract Long noncoding RNAs (lncRNAs) are known to play important roles in cancers. However, little is known about lncRNAs in cholangiocarcinoma (CCA), a cholangiocyte malignancy with poor prognosis. We investigated the role of nuclear paraspeckle assembly transcript 1 (NEAT1) lncRNA in promoting CCA. qRT-PCR analysis of patient samples showed that NEAT1 expression was higher in CCA tumors than in matched adjacent nontumor tissue. NEAT1 levels were also higher in CCA cell lines than in a normal biliary epithelium cell line (HIBEpic). NEAT1 knockdown in CCA cell lines using shNEAT1 reduced cell proliferation and colony formation in… More >

View

60

Download

44

Open Access

ARTICLE

Yuanyuan Wang^*1, Qin Luo^*1, Xianlu He^†1, He Wei^*, Ting Wang^*, Jichun Shao^‡, Xinni Jiang^*
Oncology Research, Vol.26, No.6, pp. 889-899, 2018, DOI:10.3727/096504017X15009419625178
Abstract Recent studies revealed that emodin extracted from Chinese herbs exhibits an anticancer effect on different cancer types, including colon cancer. However, the mechanism is not well understood. In our study, we confirmed that emodin treatment inhibited cell viability and induced apoptosis in colon cancer cells. Further experiments found that emodin was also able to induce autophagy, which is indispensible for apoptosis induced by emodin. More interestingly, emodin treatment also results in mitochondrial dysfunction and ROS accumulation in colon cancer cells. Finally, we stressed that ROS accumulation is essential for autophagy and apoptosis induced by emodin. More >

View

73

Download

60

Open Access

ARTICLE

Jianjun Shen^*1, Weina Niu^†1, Hongbo Zhang^*, Ma Jun^*, Hongyan Zhang^*
Oncology Research, Vol.26, No.6, pp. 901-911, 2018, DOI:10.3727/096504017X15061902533715
Abstract Gastric cancer is the fourth most common malignancy and the third leading cause of cancer-related deaths worldwide. This study aimed to investigate the expression patterns, biological roles, and underlying mechanisms of microRNA-147 (miR-147) in gastric cancer. The present study demonstrated that miR-147 was significantly upregulated in gastric cancer tissues and cell lines. Downregulation of miR-147 decreased cell proliferation and enhanced the chemosensitivity of gastric cancer cells to 5-fluorouracil (5-FU) through the cell apoptosis pathway. In addition, phosphatase and tensin homolog (PTEN) was mechanically identified as the direct target of miR-147 in gastric cancer. PTEN knockdown More >

View

50

Download

45

Open Access

ARTICLE

Jianli Chen^*1, Xiaowen Chen^†1
Oncology Research, Vol.26, No.6, pp. 913-922, 2018, DOI:10.3727/096504017X15135941182107
Abstract Breast cancer remains a public health issue on a global scale. The present study aimed to explore the functional role of MYB proto-oncogene like 2 (MYBL2) in breast cancer, as well as underlying mechanisms. The regulatory relationship between miR-143-3p and MYBL2 was analyzed, and the effects of dysregulation of miR-143-3p and MYBL2 on cell proliferation and apoptosis were investigated. The results showed that MYBL2 and miR-143-3p were inversely expressed in breast cancer tissues and cells: MYBL2 was highly expressed, whereas miR-143-3p was lowly expressed. MYBL2 was confirmed as a target gene of miR-143-3p. Suppression of More >

View

61

Download

43

Open Access

ARTICLE

Yingying Wang^*†, Yongjie Tian^*
Oncology Research, Vol.26, No.6, pp. 923-931, 2018, DOI:10.3727/096504017X15143731031009
Abstract miR-206 and Bcl-2-associated athanogene 3 (BAG3) have been suggested as important regulators in various cancer types. However, the biological role of miR-206 and BAG3 in cervical cancer (CC) remains unclear. We investigated the expressions and mechanisms of miR-206 and BAG3 in CC using in vitro and in vivo assays. In the present study, miR-206 expression was expressed at a lower level in CC tissues and cells than adjacent normal tissues and NEECs. By contrast, BAG3 mRNA and protein were expressed at higher levels in CC tissues and cells. Furthermore, miR-206 overexpression repressed cell proliferation, migration, More >

View

49

Download

68

Open Access

ARTICLE

Xuesong Wang^*, Yong Lin^†, Lei Peng^‡, Ruifu Sun^*, Xiaojin Gong^*, Jinlong Du^*, Xiugong Zhang^*
Oncology Research, Vol.26, No.6, pp. 933-940, 2018, DOI:10.3727/096504017X15144741233346
Abstract Osteosarcoma is one of the most aggressive malignancies with poor prognosis rates. Many studies have demonstrated that miRNAs were involved in osteosarcoma, but the role of miR-103a in osteosarcoma remains elusive. In this study, we detected the expression levels of miR-103 in osteosarcoma and non-osteosarcoma tissues and cell lines. The binding effect of miR-103 on p57 was detected by luciferase reporter assay. After altering expressions of miR-103 or p57, viability, migration, invasion, and apoptosis of MG63 cells and expressions of proteins related with the JNK/STAT and mTOR pathways were all detected. We found the higher More >

View

52

Download

44

Open Access

ARTICLE

Peizhen Yang^*, Dezhong Sun^*, Fei Jiang^†
Oncology Research, Vol.26, No.6, pp. 941-948, 2018, DOI:10.3727/096504018X15149775533331
Abstract Ailanthone (AIL) is a quassinoid isolated from the traditional Chinese medicinal herb Ailanthus altissima. The antitumor activities of AIL have been reported in several cancers. The purpose of the present study was to explore the effect of AIL on vestibular schwannomas (VSs). Various concentrations of AIL (0–1 µM) were used to treat human primary VS cells, and then cell viability, proliferation, apoptosis, and autophagy were assessed. Expression of miR-21 in VS cells was altered by miRNA transfection. The functional actions of AIL on miR-21 dysregulated cells were also assessed. AIL significantly reduced the viability of VS… More >

View

54

Download

41

Open Access

ARTICLE

Liqing Zhang^*, Jianjiang Xu^†, Gaodi Yang^*, Heng Li^‡, Xiuxia Guo^§
Oncology Research, Vol.26, No.6, pp. 949-957, 2018, DOI:doi.org/10.3727/096504018X15149787144385
Abstract Recent studies have demonstrated that miR-202 is associated with several types of cancer; however, the expression and function of miR-202 have not been investigated in bladder cancer. We analyzed the expression of miR-202 in bladder cancer tissues and adjacent noncancerous tissues. The effect of miR-202 on the proliferation, migration, and invasion was evaluated by in vitro assays. The target gene of miR-202 was assessed by luciferase reporter assay. In this study, miR-202 was found to be significantly downregulated in bladder cancer cell lines and tissues and was highly correlated with the T classification, N classification, More >

View

64

Download

44

Open Access

ARTICLE

Xianguang Feng^*, Wenhuan Yao^†, Zengzhen Zhang^*, Fangshui Yuan^*, Li Liang^*, Jingqiang Zhou^*, Shuang Liu^*, Jiqing Song^‡
Oncology Research, Vol.26, No.6, pp. 959-966, 2018, DOI:10.3727/096504017X15145624664031
Abstract Tbx3, a member of the T-box family of transcription factors, contributes directly to tumor formation, migration, and invasion. However, the role of Tbx3 in the metastasis of HCC remains unclear. In the present study, Tbx3 expression was detected in HCC tissues and cells by Western blot, and Tbx3 expression was regulated by use of siRNAs or lentivirus-mediated vectors. Here we found that Tbx3 protein expression increased in HCC tissues and cell lines. Tbx3 expression was positively associated with multiple tumor nodes, venous infiltration, and advanced TNM tumor stage. Survival analysis demonstrated that Tbx3 expression was… More >

View

55

Download

50

Open Access

ARTICLE

Haiyan Jia^*, Hong Wang^†, Yanfen Yao^*, Chunlei Wang^‡, Pibao Li^*
Oncology Research, Vol.26, No.6, pp. 967-976, 2018, DOI:10.3727/096504018X15148192843443
Abstract MicroRNAs (miRNAs) play a vital role in regulating tumor progression. Dysregulated miR-136 expression was linked to the development of various human cancers. In the present study, we investigated the expression and relationship of miR-136 and COX2 in hepatocellular carcinoma (HCC) using relevant experiments, involving CCK-8, Transwell assay, and luciferase reporter assay. We demonstrated that miR-136 expression is obviously decreased in HCC tissues and cells, and negatively correlated with the expression of COX2 mRNA. In vitro assay revealed that overexpression of miR-136 significantly changed the expression of proliferation- and metastasis-related proteins and inhibited the proliferation, migration, More >

View

56

Download

49

Open Access

ARTICLE WITHDRAWN

Wang Xiufen¹, Xie Yucui², Wang Jing³
Oncology Research, Vol.26, No.6, pp. 977-985, 2018, DOI:10.3727/096504017X15037506066252
Abstract THIS ARTICLE WAS WITHDRAWN BY THE PUBLISHERS IN OCTOBER 2020. More >

View

66

Download

47