Open Access
ARTICLE
Long Noncoding RNA (lncRNA) HOTAIR Affects Tumorigenesis and Metastasis of Non-Small Cell Lung Cancer by Upregulating miR-613
Caiyu Jiang, Yan Yang, Yang Yang, Lu Guo, Jiang Huang, Xingren Liu, Chi Wu, Jun Zou
Department of Respiratory and Critical Care Medicine, Sichuan Academy of Medical Sciences
and Sichuan Provincial People’s Hospital, Sichuan, P.R. China
Oncology Research 2018, 26(5), 725-734. https://doi.org/10.3727/096504017X15119467381615
Abstract
Non-small cell lung cancer (NSCLC) is one of the most deadly cancers with poor prognosis. Recent findings
suggested that the lncRNA HOTAIR played an important role in tumorigenesis and metastasis. In the present study, HOTAIR was highly expressed in NSCLC tumor tissues and cell lines (H1299, H23, H292, and
A549). Downregulation of HOTAIR suppressed cell proliferation and invasion, while it promoted apoptosis
of NSCLC cells. The targeting relationship between HOTAIR and miR-613 was first revealed by bioinformatics prediction. miR-613 was found to be lowly expressed in NSCLC tumor tissues and cell lines. Knockdown
of HOTAIR increased the expression of miR-613 significantly, and cotransfection with miR-613 inhibitor
reversed this increase, indicating that the expression of miR-613 was negatively regulated by HOTAIR. The
targeting relationship between HOTAIR and miR-613 was further confirmed through the luciferase report
assay. Moreover, the cotransfection of HOTAIR shRNA and miR-613 inhibitor counteracted the tumor inhibition effects of HOTAIR shRNA through promoting cell proliferation and invasion while suppressing apoptosis
in NSCLC cells, suggesting that the HOTAIR/miR-613 axis was involved in tumorigenesis and metastasis
of NSCLC. In vivo experiments revealed that knockdown of HOTAIR decreased tumor growth and invasion
and increased apoptosis and miR-613 expression. In conclusion, our study indicated that downregulation of
HOTAIR suppressed tumorigenesis and metastasis of NSCLC via upregulating the expression of miR-613. The
HOTAIR/miR-613 axis might provide a new potential therapeutic strategy for NSCLC treatment.
Keywords
Cite This Article
APA Style
Jiang, C., Yang, Y., Yang, Y., Guo, L., Huang, J. et al. (2018). Long noncoding RNA (lncrna) HOTAIR affects tumorigenesis and metastasis of non-small cell lung cancer by upregulating mir-613. Oncology Research, 26(5), 725-734. https://doi.org/10.3727/096504017X15119467381615
Vancouver Style
Jiang C, Yang Y, Yang Y, Guo L, Huang J, Liu X, et al. Long noncoding RNA (lncrna) HOTAIR affects tumorigenesis and metastasis of non-small cell lung cancer by upregulating mir-613. Oncol Res. 2018;26(5):725-734 https://doi.org/10.3727/096504017X15119467381615
IEEE Style
C. Jiang et al., "Long Noncoding RNA (lncRNA) HOTAIR Affects Tumorigenesis and Metastasis of Non-Small Cell Lung Cancer by Upregulating miR-613," Oncol. Res., vol. 26, no. 5, pp. 725-734. 2018. https://doi.org/10.3727/096504017X15119467381615