Open Access
ARTICLE
miR-204 Regulates Cell Proliferation and Invasion by Targeting EphB2 in Human Cervical Cancer
Shanhong Duan*, Ali Wu†, Zhengyu Chen‡, Yarong Yang*, Liying Liu*, Qi Shu*
* Department of Gynecology, Shaanxi Nuclear Industry 215 Hospital, Xianyang, Shaanxi, P.R. China
† Department of Endoscopy, Shaanxi Nuclear Industry 215 Hospital, Xianyang, Shaanxi, P.R. China
‡ Department of Spine Surgery, The First People’s Hospital of Xianyang City, Xianyang, Shaanxi, P.R. China
Oncology Research 2018, 26(5), 713-723. https://doi.org/10.3727/096504017X15016337254641
Abstract
MicroRNAs (miRNAs) are small noncoding RNAs that are involved in human carcinogenesis and progression. miR-204 has been reported to be a tumor suppressor in several cancer types. However, the function and
underlying molecular mechanism of miR-204 in cervical cancer (CC) are still unclear. In the present study, the
expression level of miR-204 was measured using the qRT-PCR method in 30 paired CC clinical samples and
in 6 CC cell lines. We found that the expression of miR-204 was significantly downregulated in CC tissues and
cell lines compared to normal cervical tissues and cell line. miR-204 was overexpressed by transfection with
the miR-204 mimic in HeLa and C33A cell lines in the following experiments. The results showed that overexpression of miR-204 dramatically suppressed cell proliferation, migration, and invasion, caused cell cycle
arrest at the G
0/G
1 phase, promoted cell apoptosis in vitro, and inhibited tumor growth in vivo. Western blot
results indicated that overexpressing miR-204 decreased the expressions of CDK2, cyclin E, MMP2, MMP9,
Bcl2, whereas it enhanced Bax expression and suppressed the activation of the PI3K/AKT signaling pathways
in CC cells. Ephrin type B receptor 2 (EphB2) was identified as a direct target of miR-204 in CC cells according to bioinformatics analysis and luciferase reporter assay. Furthermore, knockdown of EphB2 mimicked the
inhibitory effect of miR-204 on the proliferation, invasion, and migration of CC cells. These findings suggested
that miR-204 might serve as a tumor suppressor in the development of CC by directly targeting EphB2.
Keywords
Cite This Article
APA Style
Duan, S., Wu, A., Chen, Z., Yang, Y., Liu, L. et al. (2018). Mir-204 regulates cell proliferation and invasion by targeting ephb2 in human cervical cancer. Oncology Research, 26(5), 713-723. https://doi.org/10.3727/096504017X15016337254641
Vancouver Style
Duan S, Wu A, Chen Z, Yang Y, Liu L, Shu Q. Mir-204 regulates cell proliferation and invasion by targeting ephb2 in human cervical cancer. Oncol Res. 2018;26(5):713-723 https://doi.org/10.3727/096504017X15016337254641
IEEE Style
S. Duan, A. Wu, Z. Chen, Y. Yang, L. Liu, and Q. Shu "miR-204 Regulates Cell Proliferation and Invasion by Targeting EphB2 in Human Cervical Cancer," Oncol. Res., vol. 26, no. 5, pp. 713-723. 2018. https://doi.org/10.3727/096504017X15016337254641