Table of Content

Open Access iconOpen Access

ARTICLE

Long Noncoding RNA CAMTA1 Promotes Proliferation and Mobility of the Human Breast Cancer Cell Line MDA-MB-231 via Targeting miR-20b

Pengwei Lu, Yuanting Gu, Lin Li, Fang Wang, Xue Yang, Yunqing Yang

Department of Breast Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P.R. China

Oncology Research 2018, 26(4), 625-635. https://doi.org/10.3727/096504017X14953948675395

A retraction of this article was approved in:

Retraction: Long noncoding RNA CAMTA1 promotes proliferation and mobility of the human breast cancer cell line MDA-MB-231 via targeting miR-20b
Read retraction

Abstract

Breast cancer is a serious threat to women’s physical and psychological health. Long noncoding RNA CAMTA1 (lncCAMTA1) was believed to be related with tumor progression, but its role in breast cancer is not clear. The human breast cancer cell line MDA-MB-231 was used to investigate the effect of lncCAMTA1 on cell viability, migration/invasion, and apoptosis. The expression of lncCAMTA1, miR-20b, and VEGF in MDAMB-231 were measured after corresponding transfections. Binding effects between lncCAMTA1 and miR-20b, miR-20b, and VEGF 3'-UTR were measured. The effects of miR-20b and VEGF on breast cancer cells were also assessed after transfections. The phosphorylation levels of the MAPK/ERK and JAK/STAT3 pathways were determined to assess the effect of VEGF. The results showed that lncCAMTA1 expression promoted cell viability and migration/invasion, while knockdown of lncCAMTA1 promoted cell apoptosis via binding with miR-20b. lncCAMTA1 negatively regulated miR-20b expression. VEGF was a target of miR-20b, leading to the modification of the phosphorylation levels of MAPK, ERK, JAK, STAT1, and STAT3. Our findings suggested that lncCAMTA1 might promote proliferation and mobility of human breast cancer cells via binding with miR-20b. VEGF was a direct target of miR-20b and regulated activation of the MAPK/ERK and JAK/ STAT3 signaling pathways. Therefore lncCAMTA1 has potential as a novel cancer diagnostic marker and as a putative novel therapeutic target for breast cancer treatment.

Keywords


Cite This Article

APA Style
Lu, P., Gu, Y., Li, L., Wang, F., Yang, X. et al. (2018). Long noncoding RNA CAMTA1 promotes proliferation and mobility of the human breast cancer cell line MDA-MB-231 via targeting mir-20b. Oncology Research, 26(4), 625-635. https://doi.org/10.3727/096504017X14953948675395
Vancouver Style
Lu P, Gu Y, Li L, Wang F, Yang X, Yang Y. Long noncoding RNA CAMTA1 promotes proliferation and mobility of the human breast cancer cell line MDA-MB-231 via targeting mir-20b. Oncol Res. 2018;26(4):625-635 https://doi.org/10.3727/096504017X14953948675395
IEEE Style
P. Lu, Y. Gu, L. Li, F. Wang, X. Yang, and Y. Yang, “Long Noncoding RNA CAMTA1 Promotes Proliferation and Mobility of the Human Breast Cancer Cell Line MDA-MB-231 via Targeting miR-20b,” Oncol. Res., vol. 26, no. 4, pp. 625-635, 2018. https://doi.org/10.3727/096504017X14953948675395



cc Copyright © 2018 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 223

    View

  • 114

    Download

  • 0

    Like

Share Link