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ARTICLE
MicroRNA-520b Functions as a Tumor Suppressor in Colorectal Cancer by Inhibiting Defective in Cullin Neddylation 1 Domain Containing 1 (DCUN1D1)
Jing Xiao*, Guang Li†, Jingyu Zhou‡, Shalong Wang‡, Dongcai Liu‡, Guoshun Shu‡, Jianping Zhou‡, Feng Ren‡
* Minimally Invasive Surgery Center, The Second Xiangya Hospital of Central South University, Changsha, Hunan, P.R. China
† Department of Gastrointestinal Surgery, People’s Hospital of Linyi City, Linyi, Shandong, P.R. China
‡ Department of Gerontological Surgery, The Second Xiangya Hospital of Central South University, Changsha, Hunan, P.R. China
Oncology Research 2018, 26(4), 593-604. https://doi.org/10.3727/096504017X14920318811712
Abstract
MicroRNAs (miRs), a class of small noncoding RNAs, are important regulators for gene expression through
directly binding to the 3'-untranslated region (3'-UTR) of their target mRNA. Recently, downregulation of
miR-520b has been observed in several common human cancers. However, the exact role of miR-520b in
colorectal cancer (CRC) has not previously been studied. In this study, our data showed that miR-520b was
significantly downregulated in CRC and cell lines when compared with adjacent normal tissues and a normal
intestinal epithelial cell line. Low expression of miR-520b was notably associated with the malignant progress
and a shorter survival time for CRC patients. Restoration of miR-520b inhibited cell proliferation, migration,
invasion, and epithelial–mesenchymal transition (EMT) in CRC cells. Defective in cullin neddylation 1 domain
containing 1 (DCUN1D1) was then identified as a novel target gene of miR-520b in CRC cells. The expression of DCUN1D1 was significantly increased in CRC, with a negative correlation to miR-520b expression
in CRC tissues. Moreover, a high expression of DCUN1D1 was significantly associated with the malignant
progress and a poor prognosis for CRC patients. Furthermore, overexpression of DCUN1D1 rescued the miR-
520b-mediated malignant phenotypes and EMT in CRC cells. The data demonstrate that miR-520b functions
as a tumor suppressor in CRC through targeting DCUN1D1, suggesting that miR-520b may become a potential
therapeutic target for the treatment of CRC.
Keywords
Cite This Article
APA Style
Xiao, J., Li, G., Zhou, J., Wang, S., Liu, D. et al. (2018). Microrna-520b functions as a tumor suppressor in colorectal cancer by inhibiting defective in cullin neddylation 1 domain containing 1 (DCUN1D1). Oncology Research, 26(4), 593-604. https://doi.org/10.3727/096504017X14920318811712
Vancouver Style
Xiao J, Li G, Zhou J, Wang S, Liu D, Shu G, et al. Microrna-520b functions as a tumor suppressor in colorectal cancer by inhibiting defective in cullin neddylation 1 domain containing 1 (DCUN1D1). Oncol Res. 2018;26(4):593-604 https://doi.org/10.3727/096504017X14920318811712
IEEE Style
J. Xiao et al., "MicroRNA-520b Functions as a Tumor Suppressor in Colorectal Cancer by Inhibiting Defective in Cullin Neddylation 1 Domain Containing 1 (DCUN1D1)," Oncol. Res., vol. 26, no. 4, pp. 593-604. 2018. https://doi.org/10.3727/096504017X14920318811712