Open Access
ARTICLE
Long Noncoding RNA ANRIL Promotes Cervical Cancer Development by Acting as a Sponge of miR-186
Jun-Jun Zhang*, Dan-Dan Wang*, Chen-Xiang Du†, Yan Wang‡
* Department of Obstetrics and Gynecology, The First Affiliated Hospital of Henan University of Science and Technology,
Luoyang, Henan Province, P.R. China
† Department of Obstetrics, People’s Hospital of Zhengzhou University, Zhengzhou, Henan Province, P.R. China
‡ Department of Gynecology, The First Affiliated Hospital of Henan University of Science and Technology in The New Area,
Luoyang, Henan Province, P.R. China
Oncology Research 2018, 26(3), 345-352. https://doi.org/10.3727/096504017X14953948675449
Abstract
Cervical cancer is a common malignancy of the female reproductive system. Long noncoding RNAs (lncRNAs)
have been reported to modulate tumor progression in multiple cancers. The lncRNA antisense noncoding RNA
in the INK4 locus (ANRIL) has been identified as an oncogenic molecular target in several tumors; however,
the function and underlying mechanism involved in cervical cancer oncogenesis are still unclear. In the present
study, RT-PCR showed that ANRIL expression was significantly upregulated in cervical cancer tumors and
cell lines. Nevertheless, ANRIL knockdown transfected with interference oligonucleotide inhibited the proliferation activity and invasive ability, and promoted apoptosis of cervical cancer cell lines. The bioinformatics
prediction program and luciferase assay predicted and validated that miR-186 directly targeted ANRIL. The
expression level of miR-186 was downregulated in cervical cancer tumors and cell lines and was negatively
correlated to that of ANRIL. Moreover, rescue experiments showed that miR-186 inhibitor could reverse the
suppression of ANRIL knockdown. In summary, our study demonstrated that the ANRIL/miR-186 axis might
play a vital role in cervical cancer tumorigenesis.
Keywords
Cite This Article
APA Style
Zhang, J., Wang, D., Du, C., Wang, Y. (2018). Long noncoding RNA ANRIL promotes cervical cancer development by acting as a sponge of mir-186. Oncology Research, 26(3), 345-352. https://doi.org/10.3727/096504017X14953948675449
Vancouver Style
Zhang J, Wang D, Du C, Wang Y. Long noncoding RNA ANRIL promotes cervical cancer development by acting as a sponge of mir-186. Oncol Res. 2018;26(3):345-352 https://doi.org/10.3727/096504017X14953948675449
IEEE Style
J. Zhang, D. Wang, C. Du, and Y. Wang "Long Noncoding RNA ANRIL Promotes Cervical Cancer Development by Acting as a Sponge of miR-186," Oncol. Res., vol. 26, no. 3, pp. 345-352. 2018. https://doi.org/10.3727/096504017X14953948675449