Open Access

ARTICLE

MicroRNA-433 Represses Proliferation and Invasion of Colon Cancer Cells by Targeting Homeobox A1

Heming Li^*1, Junfeng Li^*1, Taisheng Yang^*, Shuwen Lin^†, Heng Li^‡

* Emergency Department, 5th Hospital of Dongguan City, Dongguan, P.R. China
† Hepatobiliary Surgery Department, 5th Hospital of Dongguan City, Dongguan, P.R. China
‡ Cardiovascular Department, TungWah Hospital of Sun-Yat Sen University, Donggguan, P.R. China
1 These authors provided equal contribution to this work.

Oncology Research 2018, 26(2), 315-322. https://doi.org/10.3727/096504017X15067856789781

Abstract

The aberrant expression of miR-433 has been validated in some types of cancers. However, the expression profile and the biological function of miR-433 on colon cancer are still elusive. This study was designed to investigate the function of miR-433 on the proliferation and invasion of colon cancer cells. We detected the expression of miR-433 in colon cancer tissues, adjacent normal tissues, and cell lines. CCK8 and Transwell assays were performed to explore the impact of miR-433 on colon cancer cell proliferation and invasion. The luciferase reporter assay was applied to identify the direct target of miR-433. The results demonstrated that miR-433 was downregulated in colon cancer tissues and cell lines when compared with the control. Overexpression of miR- 433 significantly suppressed the ability of colon cancer cell proliferation and invasion, whereas knockdown of miR-433 remarkably enhanced cell proliferation and invasion. Homeobox A1 (HOXA1) was identified as a target of miR-433, and it mediated the functions of miR-433 on colon cancer cells. To conclude, we revealed that miR-433 was downregulated in colon cancer, and it inhibited colon cancer cell proliferation and invasion by directly targeting HOXA1.

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