Open Access
ARTICLE
MicroRNA-186 Suppresses Cell Proliferation and Metastasis Through Targeting Sentrin-Specific Protease 1 in Renal Cell Carcinoma
Dan Jiao*, Man Wu†, Lei Ji‡, Feng Liu§, Yingying Liu§
* Department of Ultrasound, China–Japan Union Hospital of Jilin University, Changchun, Jilin, P.R. China
† Department of Nephrology, The Second Hospital of Jilin University, Changchun, Jilin, P.R. China
‡ Department of Cardiology, Changchun Central Hospital, Changchun, Jilin, P.R. China
§ Department of Nephrology, China–Japan Union Hospital of Jilin University, Changchun, Jilin, P.R. China
Oncology Research 2018, 26(2), 249-259. https://doi.org/10.3727/096504017X14953948675430
Abstract
Recent evidence suggests that dysregulation of microRNAs is associated with the development of multiple
malignancies. miR-186 has been reported as a critical cancer regulator in several types of cancers. However,
its functional significance and molecular mechanism underlying renal cell carcinoma (RCC) remain unknown.
In this study, our results showed that miR-186 expression was dramatically downregulated in RCC tissues and
cell lines compared to that in adjacent normal tissues and cell lines. Overexpression of miR-186 significantly
inhibited cell growth, colony formation, and cell invasion; caused cell cycle arrest at the G
0/G
1 phase; and
induced cell apoptosis as detected by MTT, colony formation, Transwell assay, and flow cytometry assays in
RCC cells. In addition, inhibition of miR-186 expression promoted RCC cell proliferation, invasion, and cell
cycle progression and reduced apoptosis. Bioinformatics analysis and luciferase reporter assay confirmed that
the 3'-UTR of sentrin-specific protease 1 (SENP1) was a direct target of miR-186. A remarkably reverse correlation was observed between miR-186 and SENP1 mRNA in RCC tissues. Furthermore, immunohistochemical
staining revealed that SENP1 was positively expressed in RCC specimens. Restoration of SENP1 expression
could partially abrogate the inhibitory effect of miR-186 overexpression on RCC cell proliferation through activating NF-kB signaling and its downstream proteins. These data demonstrated that miR-186 acted as a novel
tumor suppressor and potential therapeutic biomarker in the progression of RCC by directly targeting SENP1.
Keywords
Cite This Article
APA Style
Jiao, D., Wu, M., Ji, L., Liu, F., Liu, Y. (2018). Microrna-186 suppresses cell proliferation and metastasis through targeting sentrin-specific protease 1 in renal cell carcinoma. Oncology Research, 26(2), 249-259. https://doi.org/10.3727/096504017X14953948675430
Vancouver Style
Jiao D, Wu M, Ji L, Liu F, Liu Y. Microrna-186 suppresses cell proliferation and metastasis through targeting sentrin-specific protease 1 in renal cell carcinoma. Oncol Res. 2018;26(2):249-259 https://doi.org/10.3727/096504017X14953948675430
IEEE Style
D. Jiao, M. Wu, L. Ji, F. Liu, and Y. Liu "MicroRNA-186 Suppresses Cell Proliferation and Metastasis Through Targeting Sentrin-Specific Protease 1 in Renal Cell Carcinoma," Oncol. Res., vol. 26, no. 2, pp. 249-259. 2018. https://doi.org/10.3727/096504017X14953948675430