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Yingchao Li^*, Xiaoni Yan^*, Li Ren^*, Yang Li^†
Oncology Research, Vol.26, No.1, pp. 1-8, 2018, DOI:10.3727/096504016X14772410356982
Abstract Epithelial–mesenchymal transition (EMT) is one of the most important mechanisms in the metastasis of various cancers, including gastric cancer (GC). In this study, we explored the putative significance of miR-644a and its role in EMT-mediated metastasis of GC. We first detected the expression of miR-644a in a cohort of 107 GC tissues using quantitative RT-PCR. The expression of miR-644a was suppressed in GC tissues and was associated with a later clinical stage and tumor metastasis. Restoring the expression of miR-644a could significantly suppress the migration and invasion of HGC-27 and SGC-7901 cells, which might be More >

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Yuan Yang^*, Lingli Quan^†, Ye Ling^*
Oncology Research, Vol.26, No.1, pp. 9-15, 2018, DOI:10.3727/096504017X14871200709504
Abstract RBMS3, a gene encoding a glycine-rich RNA-binding protein, belongs to the family of c-Myc gene singlestrand binding proteins (MSSP). Recently, several reports have provided evidence that RBMS3 was deregulated in a diverse range of solid tumors and played a critical role in tumor progression. However, it remains unclear whether RBMS3 inhibits the progression of human breast cancer. Thus, the aim of this study was to investigate the role of RBMS3 in breast cancer and explore the underlying mechanism in breast cancer progression. Our results showed, for the first time, that the expression of RBMS3 at… More >

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Vinitha Richard, Rajesh Raju, Aswathy Mary Paul, Reshmi Girijadevi, Thankayyan Retnabai Santhosh Kumar, Madhavan Radhakrishna Pillai
Oncology Research, Vol.26, No.1, pp. 17-26, 2018, DOI:10.3727/096504017X14881490607028
Abstract This study is an integrated analysis of the transcriptome profile microRNA (miRNA) and its experimentally validated mRNA targets differentially expressed in the tumorigenic stem-like fraction of oral squamous cell carcinoma (OSCC). We had previously reported the coexistence of multiple drug-resistant tumorigenic fractions, termed side population (SP1, SP2, and MP2), and a nontumorigenic fraction, termed main population (MP1), in oral cancer. These fractions displayed a self-renewal, regenerative potential and expressed known stemness-related cell surface markers despite functional differences. Flow cytometrically sorted pure fractions of SP1 and MP1 cells were subjected to differential expression analysis of both… More >

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Ling Li, He-Sheng Luo
Oncology Research, Vol.26, No.1, pp. 27-35, 2018, DOI:10.3727/096504017X14900515946914
Abstract Colorectal carcinoma is one of the leading causes of cancer-related deaths and has a high tendency for metastasis, which makes it a priority to find novel methods to diagnose and treat colorectal carcinoma at a very early stage. We studied the role of the regulator of G-protein signaling (RGS) family of proteins RGS17 in colorectal carcinoma growth and metastasis. We found that RGS17 was upregulated in both clinical colorectal carcinoma tissues and cultured colorectal carcinoma cells. Knockdown of RGS17 by specific siRNA decreased the cell proliferation rate, whereas overexpression of RGS17 with expression plasmid increased More >

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Bin Zhang¹, Na Li¹, Hao Zhang
Oncology Research, Vol.26, No.1, pp. 37-44, 2018, DOI:10.3727/096504017X14900530835262
Abstract Homeobox B5 (HOXB5), a member of the HOX gene family, has been shown to play an important role in tumor progression. However, the expression and functional role of HOXB5 in human non-small cell lung cancer (NSCLC) have not been defined. Thus, the purpose of this study was to elucidate the expression and functional role of HOXB5 in human NSCLC. Our results showed that HOXB5 expression was elevated in human NSCLC tissues and cell lines. The in vitro experiments demonstrated that knockdown of HOXB5 inhibited proliferation, migration, and invasion and prevented the EMT phenotype in NSCLC More >

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Naoki Katase^*†, Shin-Ichiro Nishimatsu^†‡, Akira Yamauchi^§, Masahiro Yamamura^¶, Kumiko Terada^†‡, Masumi Itadani^§, Naoko Okada^¶#, Nur Mohammad Monsur Hassan^**, Hitoshi Nagatsuka^††, Tohru Ikeda^*‡‡, Tsutomu Nohno^†, Shuichi Fujita^*
Oncology Research, Vol.26, No.1, pp. 45-58, 2018, DOI:10.3727/096504017X14926874596386
Abstract DKK3, a member of the dickkopf Wnt signaling pathway inhibitor family, is believed to be a tumor suppressor because of its reduced expression in cancer cells. However, our previous studies have revealed that DKK3 expression is predominantly observed in head and neck/oral squamous cell carcinoma (HNSCC/OSCC). Interestingly, HNSCC/OSCC patients with DKK3 expression showed a high rate of metastasis and poorer survival, and siRNA-mediated knockdown of DKK3 in HNSCC-derived cancer cell lines resulted in reduced cellular migration and invasion. From these data, it was hypothesized that DKK3 might exert an oncogenic function specific to HNSCC. In… More >

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Ilias Kainis^*, Nikolaos Syrigos^*, Alexandra Kopitopoulou^*, Ioannis Gkiozos^*, Effrosyni Filiou^*, Vasiliki Nikolaou^*, Evangelia Papadavid^†
Oncology Research, Vol.26, No.1, pp. 59-69, 2018, DOI:10.3727/096504017X14913452320194
Abstract Systematic treatment of advanced non-small cell lung cancer (NSCLC) includes targeted treatment with epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs). The development of skin rash and its intensity have been associated with EGFR TKI’s efficacy. The main purpose of this study was to further investigate the potential value of erlotinib-associated rash as a predictor of prognosis and treatment response in a real-world cohort of patients with advanced NSCLC. The medical records of all NSCLC patients treated with erlotinib at the Oncology Unit of GPP, Sotiria Athens General Hospital between January 1, 2014 and… More >

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Jeong Yong Jeon^*1, Misu Lee^*†1, Sang Hyun Whang^*, Jung-Whan Kim^‡, Arthur Cho^*, Mijin Yun^*
Oncology Research, Vol.26, No.1, pp. 71-81, 2018, DOI:10.3727/096504017X14902648894463
Abstract Altered energy metabolism is a biochemical fingerprint of cancer cells. Hepatocellular carcinoma (HCC) shows reciprocal [¹⁸F]fluorodeoxyglucose (FDG) and [¹¹C]acetate uptake, as revealed by positron emission tomography/computed tomography (PET/CT). Previous studies have focused on the role of FDG uptake in cancer cells. In this study, we evaluated the mechanism and roles of [¹¹C]acetate uptake in human HCCs and cell lines. The expression of monocarboxylate transporters (MCTs) was assessed to determine the transporters of [¹¹C]acetate uptake in HCC cell lines and human HCCs with different [¹¹C]acetate uptake. Using two representative cell lines with widely different [¹¹C]acetate uptake (HepG2 for high… More >

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Ce Ji^*, Ying Zhao^*, You-Wei Kou^*, Hua Shao^*, Lin Guo^*, Chen-Hui Bao^*, Ben-Chun Jiang^*, Xin-Ying Chen^*, Jing-Wei Dai^†, Yu-Xin Tong^‡, Ren Yang^*, Wei Sun^*, Qiang Wang^*
Oncology Research, Vol.26, No.1, pp. 83-93, 2018, DOI:10.3727/096504017X14928634401204
Abstract Gastric cancer (GC) is one of the most common cancers in the world. The cathepsin F (CTSF) gene has recently been found to participate in the progression of several types of cancer. However, the clinical characteristics and function of CTSF in GC as well as its molecular mechanisms are not clear. Six GC cell lines and 44 paired adjacent noncancerous and GC tissue samples were used to assess CTSF expression by quantitative polymerase chain reaction (qPCR). We used lentivirus-mediated small hairpin RNA (Lenti-shRNA) against CTSF to knock down the expression of CTSF in GC cells.… More >

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Jun-Ying Wang^*, Xin Jin^*, Xiao-Feng Li^†
Oncology Research, Vol.26, No.1, pp. 95-101, 2018, DOI:10.3727/096504017X14920318811695
Abstract TMPRSS3 belongs to the large type II transmembrane serine protease (TTSP) family, which plays an important role in the development and progression of tumors. However, the function of TMPRSS3 in nasopharyngeal carcinoma (NPC) remains unclear. The present study aimed to examine the impact of TMPRSS3 on the proliferation, migration, and invasion of NPC cells and their potential mechanisms. Our results demonstrated that the expression of TMPRSS3 was obviously upregulated in human NPC tissues and cell lines. Knockdown of TMPRSS3 expression significantly suppressed the proliferation and tumorigenicity of NPC cells in vitro and in vivo. Furthermore, More >

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Yang Sun^*1, Jun-Gong Jin^*1, Wei-Yang Mi^†, Hao-Wu^*, Shi-Rong Zhang^‡, Qiang Meng^*, Shi-Tao Zhang^‡
Oncology Research, Vol.26, No.1, pp. 103-110, 2018, DOI:10.3727/096504017X14934860122864
Abstract Glioma is the most common and lethal malignant intracranial tumor. Long noncoding RNAs (lncRNAs) have been identified as pivotal regulators in the tumorigenesis of glioma. However, the role of lncRNA urothelial carcinoma-associated 1 (UCA1) in glioma genesis is still unknown. The purpose of this study was to investigate the underlying function of UCA1 on glioma genesis. The results demonstrated that UCA1 was upregulated in glioma tissue and indicated a poor prognosis. UCA1 knockdown induced by si-UCA1 significantly suppressed the proliferative, migrative, and invasive activities of glioma cell lines (U87 and U251). Bioinformatics analysis and luciferase More >

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Ruifang Zeng, Guolin Tan, Wei Li, Yanhong Ma
Oncology Research, Vol.26, No.1, pp. 111-122, 2018, DOI:10.3727/096504017X14924753593574
Abstract This study aimed to investigate the clinical significance of cullin 3 expression in nasopharyngeal carcinoma (NPC), as well as to explore the regulatory mechanism of cullin 3 underlying the growth and metastasis of NPC cells. Our findings showed that the expression levels of cullin 3 were significantly increased in both NPC tissues and cell lines. A strong positive correlation was found between cullin 3 expression and the Ki-67-based proliferation index in NPC tissues. Moreover, cullin 3 overexpression was correlated with local relapse and distant metastasis in NPC patients. In vitro experiments showed that knockdown of More >

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Yinghua Li^*†, Songyi Li^†, Lili Huang^*
Oncology Research, Vol.26, No.1, pp. 123-130, 2018, DOI:10.3727/096504017X14912172235777
Abstract Rap2B, belonging to the Ras superfamily, has been implicated in cancer development and functions as a tumor promoter. However, the role of Rap2B in cervical cancer is unknown. In this study, we investigated the expression pattern and biological functions of Rap2B in cervical cancer. The results showed that Rap2B was overexpressed in cervical cancer tissues and cell lines. Knockdown of Rap2B inhibited the proliferation, migration, and invasion of cervical cancer cells. In addition, our tumorigenesis assay showed that Rap2B knockdown suppressed cervical cancer cell growth and metastasis in vivo. We also found that the ERK1/2 More >

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Ye Li^*†‡, Haihong Luo^§, Nan Xiao^*, Jianmin Duan^*, Zhiping Wang^*, Shuanke Wang^¶
Oncology Research, Vol.26, No.1, pp. 131-143, 2018, DOI:10.3727/096504017X14944585873631
Abstract Prostate cancer has become the most commonly diagnosed and the second leading cause of cancer-related deaths in males. The long noncoding RNA second chromosome locus associated with prostate-1 (SChLAP1) has been found to be overexpressed in a subset of prostate cancer. However, the significance and mechanism of SChLAP1 in prostate cancer are not well known. In this study, we explored the role of SChLAP1 in prostate cancer tissues, cell lines, and mouse models. The effect of SChLAP1 on miR-198 and MAPK1 was specifically examined. We found that SChLAP1 expression was significantly increased in prostate cancer… More >

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Rong Wang, Yunfeng Wu, Weihua Huang, Weijun Chen
Oncology Research, Vol.26, No.1, pp. 145-155, 2018, DOI:10.3727/096504017X14902261600566
Abstract In this report, we aimed to explore the role and regulatory mechanism of microRNA-940 (miR-940) in bladder cancer development. The expressions of miR-940 in bladder cancer tissues and cells were measured. miR-940 mimics, miR-940 inhibitor small interference RNA against INPP4A (si-INPP4A), and GSK3b (si-GSK3b) and their corresponding controls were then transfected into cells. We investigated the effects of miR-940, INPP4A, or GSK3b on cell proliferation, migration, invasion, and apoptosis. Additionally, target prediction and luciferase reporter assays were performed to investigate the targets of miR-940. The regulatory relationship between miR-940 and the Wnt/β-catenin pathway was also… More >

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Yinfang Wu^*, Jun Zhang^†, Yu Zheng^‡, Cheng Ma^‡, Xing-E Liu^§, Xiaodong Sun^*‡
Oncology Research, Vol.26, No.1, pp. 157-171, 2018, DOI:10.3727/096504017X15031557924150
Abstract This work aims to elucidate the effects and the potential underlying mechanisms of microRNA-216a-3p (miR- 216a-3p) on the proliferation, migration, and invasion of gastric cancer (GC) cells. In this study, we revealed that the expression of miR-216a-3p was significantly elevated in GC tissues and cell lines. The different expression level of miR-216a-3p was firmly correlated with clinicopathological characteristics of GC patients. We next demonstrated that upregulation of miR-216a-3p could dramatically promote the ability of proliferation, migration, and invasion of GC cells using a series of experiments, whereas downregulation essentially inhibited these properties. Additionally, through bioinformatics More >

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