Ovarian cancer is highly malignant with a gradually increasing incidence and a high mortality rate. Immunosuppression is induced in ovarian cancer, although the mechanism detail is not clear. It has been indicated that
HVEM (herpesvirus entry mediator) B- and T-lymphocyte attenuator (BTLA) negatively regulates the immune
responses of T lymphocytes. Here, HVEM mRNA was found to be elevated in ovarian cancer tissue samples
and primary ovarian cancer cells in comparison with benign tissue samples. We then knocked down HVEM
expression in an ovarian cancer cell line, OVCAR3, by lentivirus-based small hairpin RNA (shRNA). Cell
Counting Kit-8 (CCK-8) assay and flow cytometry analysis showed that HVEM-shRNA had no effect on the
proliferation, early apoptosis, or cell cycle distribution of OVCAR3. We then isolated activated T cells and
performed coculture experiments in Transwell. Remarkably, HVEM-silenced ovarian cancer cells (primary
ovarian cancer cells and OVCAR3) increased the number of T cells and the secretion of tumor necrosis factor-a
(TNF-α) and interferon-γ (IFN-γ), while activated T cells promoted the apoptosis of HVEM-silenced ovarian
cancer cells. The current study partially explains the immune escape mechanism of ovarian cancer cells and
provides a possible target for immunotherapy.
Cite This Article
APA Style
Zhang, T., Ye, L., He, Q., Zhu, J. (2017). Knockdown of HVEM, a lymphocyte regulator gene, in ovarian cancer cells increases sensitivity to activated T cells. Oncology Research, 25(9), 1665-1665. https://doi.org/10.3727/096504017X15078984695565
Vancouver Style
Zhang T, Ye L, He Q, Zhu J. Knockdown of HVEM, a lymphocyte regulator gene, in ovarian cancer cells increases sensitivity to activated T cells. Oncol Res. 2017;25(9):1665-1665 https://doi.org/10.3727/096504017X15078984695565
IEEE Style
T. Zhang, L. Ye, Q. He, and J. Zhu "Knockdown of HVEM, a Lymphocyte Regulator Gene, in Ovarian Cancer Cells Increases Sensitivity to Activated T Cells," Oncol. Res., vol. 25, no. 9, pp. 1665-1665. 2017. https://doi.org/10.3727/096504017X15078984695565