Open Access
ARTICLE
UCA1 Regulates the Growth and Metastasis of Pancreatic Cancer by Sponging miR-135a
Xiaobo Zhang*, Feng Gao*, Lei Zhou*, Huaitao Wang*, Gang Shi†, Xiaodong Tan*
* Department of General Surgery, Affiliated Shengjing Hospital, China Medical University, Liaoning, P.R. China
† Department of Colorectal Surgery, Liaoning Cancer Hospital, Liaoning, P.R. China
Oncology Research 2017, 25(9), 1529-1541. https://doi.org/10.3727/096504017X14888987683152
Abstract
Pancreatic cancer (PC) is a devastating malignant disease with a poor prognosis. This study aimed to investigate the role of urothelial carcinoma associated 1 (UCA1) in the progression of PC. Our results revealed that
long noncoding RNA (lncRNA) UCA1 was overexpressed in PC tissues compared with adjacent histologically normal tissues. A downregulated level of UCA1 was also detected in five human PC cell lines (SW1990,
BxPC-3, MiaPaCa-2, PANC-1, and CAPAN-1) compared with normal pancreatic duct epithelial HPDE cells.
The proliferation of PC cells was inhibited after UCA1 was suppressed by a lentiviral vector. The cell apoptosis
rate was largely promoted by downregulating UCA1. Further research revealed that microRNA (miRNA)-135a
is a direct target of UCA1. The expression of miR-135a was decreased in PC tissues and cell lines compared
with control groups. In addition, the decreased level of miR-135a was elevated by adding miR-135a mimic in
SW1990 cells transfected with lncRNA UCA1. Similarly, an upregulated level of miR-135a was downregulated by adding miR-135a inhibitor in SW1990 cells transfected with UCA1 siRNA. Luciferase activity assay
further confirmed the targeting relationship between UCA1 and miR-135a. Moreover, miR-135a reversed the
effect of UCA1 on cell apoptosis rate and cell viability in SW1990 cells. The migration and invasion capacities
of PC cells were suppressed by UCA1. siRNA was then enhanced by the miR-135a inhibitor. In vivo, UCA1
siRNA effectively suppressed tumor growth and the expression of migration markers. Taken together, our
research revealed that UCA1 works as an oncogene by targeting miR-135a. The UCA1–miR-135a pathway
regulated the growth and metastasis of PC, providing new insight in the treatment of PC.
Keywords
Cite This Article
APA Style
Zhang, X., Gao, F., Zhou, L., Wang, H., Shi, G. et al. (2017). UCA1 regulates the growth and metastasis of pancreatic cancer by sponging mir-135a. Oncology Research, 25(9), 1529-1541. https://doi.org/10.3727/096504017X14888987683152
Vancouver Style
Zhang X, Gao F, Zhou L, Wang H, Shi G, Tan X. UCA1 regulates the growth and metastasis of pancreatic cancer by sponging mir-135a. Oncol Res. 2017;25(9):1529-1541 https://doi.org/10.3727/096504017X14888987683152
IEEE Style
X. Zhang, F. Gao, L. Zhou, H. Wang, G. Shi, and X. Tan "UCA1 Regulates the Growth and Metastasis of Pancreatic Cancer by Sponging miR-135a," Oncol. Res., vol. 25, no. 9, pp. 1529-1541. 2017. https://doi.org/10.3727/096504017X14888987683152