Open Access
ARTICLE
Overexpression of T-box Transcription Factor 5 (TBX5) Inhibits Proliferation and Invasion in Non-Small Cell Lung Carcinoma Cells
Ruoting Ma*†, Yu Yang*, Qiuyun Tu†, Ke Hu‡
* Department of Senile Disease, The Second Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China
† Department of Senile Disease, The Third Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China
‡ Department of Clinical Medicine, Hunan University of Medicine, Changsha, Hunan, P.R. China
Oncology Research 2017, 25(9), 1495-1504. https://doi.org/10.3727/096504017X14883287513729
Abstract
T-box transcription factor 5 (TBX5), a member of the conserved T-box transcription factor family that functions in organogenesis and embryogenesis, has recently been identified as a critical player in cancer development. The aim of this study was to determine the role of TBX5 in non-small cell lung carcinoma (NSCLC).
Immunohistochemistry was used to detect the correlation between levels of TBX5 and clinicopathological
features of NSCLC patients in tissue microarray. Expression of TBX5 in NSCLC tissues and cell lines was
evaluated by quantitative PCR and Western blot. The role of TBX5 in regulating proliferation, colony formation, invasion, and apoptosis of NSCLC cells was evaluated in vitro. Finally, a tumorigenicity assay was
performed to determine the effect of TBX5 on tumor growth in vivo. The levels of TBX5 in NSCLC tissues
were significantly correlated with the TNM stage (
p = 0.016), histopathologic type (
p = 0.029), and lymph node
status (
p = 0.035) of NSCLC. TBX5 overexpression markedly suppressed in vitro NSCLC cell proliferation,
colony formation, and invasion and induced apoptosis. In vivo tumor growth was significantly suppressed by
TBX5. TBX5 has a tumor-suppressing effect in NSCLC and may serve as a therapeutic target for diagnoses
and treatment of NSCLC.
Keywords
Cite This Article
APA Style
Ma, R., Yang, Y., Tu, Q., Hu, K. (2017). Overexpression of t-box transcription factor 5 (TBX5) inhibits proliferation and invasion in non-small cell lung carcinoma cells. Oncology Research, 25(9), 1495-1504. https://doi.org/10.3727/096504017X14883287513729
Vancouver Style
Ma R, Yang Y, Tu Q, Hu K. Overexpression of t-box transcription factor 5 (TBX5) inhibits proliferation and invasion in non-small cell lung carcinoma cells. Oncol Res. 2017;25(9):1495-1504 https://doi.org/10.3727/096504017X14883287513729
IEEE Style
R. Ma, Y. Yang, Q. Tu, and K. Hu "Overexpression of T-box Transcription Factor 5 (TBX5) Inhibits Proliferation and Invasion in Non-Small Cell Lung Carcinoma Cells," Oncol. Res., vol. 25, no. 9, pp. 1495-1504. 2017. https://doi.org/10.3727/096504017X14883287513729