Open Access
ARTICLE
Gastrin Enhances Autophagy and Promotes Gastric Carcinoma Proliferation via Inducing AMPKα
Zhuang Kun*†, Guo Hanqing*, Tang Hailing*, Yan Yuan*, Zhang Jun†, Zhang Lingxia*, Han Kun*, Zhang Xin*
* Division of Gastroenterology, Xi’an Central Hospital, Xi’an, P.R. China
† Division of Gastroenterology, The Second Affiliated Hospital Xi’an Jiaotong University, Xi’an, P.R. China
Oncology Research 2017, 25(8), 1399-1407. https://doi.org/10.3727/096504016X14823648620870
Abstract
Gastric cancer (GC) is one of the most frequent epithelial malignancies worldwide. The gastrointestinal (GI)
peptide gastrin is an important regulator of the secretion and release of gastric acid from stomach parietal
cells, and it also plays a vital role in the development and progression of GC. The aim of the current study
was to investigate the role and underlying mechanism of gastrin and autophagy in regulating GC tumorigenesis. Gastrin-17 amide (G-17) was applied in the GC cell lines SGC7901 and MGC-803. The results showed
that G-17 maintained the high viability of SGC7901 and MGC-803. The expression of autophagy marker
proteins LC3II and Beclin1 was significantly increased, while the autophagy substrate p62 was obviously
decreased in the gastrin group compared with the control group. Moreover, G-17 strengthened the expressions
of AMPKα, Ras, Raf, MEK, and ERK1/2. Additionally, administration of AMPKα siRNA counteracted the
effect of gastrin in SGC7901 cells. Finally, in an in vivo study of the tumor growth and survival rate of rats, the
levels of AMPKα/Ras/Raf/MEK/ERK were significantly increased in the gastrin group and decreased following AMPKα shRNA injection. In conclusion, these findings indicate that gastrin plays a tumorigenic role by
promoting autophagy in GC and may provide a novel therapeutic target for GC treatment.
Keywords
Cite This Article
APA Style
Kun, Z., Hanqing, G., Hailing, T., Yuan, Y., Jun, Z. et al. (2017). Gastrin enhances autophagy and promotes gastric carcinoma proliferation via inducing AMPKα. Oncology Research, 25(8), 1399-1407. https://doi.org/10.3727/096504016X14823648620870
Vancouver Style
Kun Z, Hanqing G, Hailing T, Yuan Y, Jun Z, Lingxia Z, et al. Gastrin enhances autophagy and promotes gastric carcinoma proliferation via inducing AMPKα. Oncol Res. 2017;25(8):1399-1407 https://doi.org/10.3727/096504016X14823648620870
IEEE Style
Z. Kun et al., "Gastrin Enhances Autophagy and Promotes Gastric Carcinoma Proliferation via Inducing AMPKα," Oncol. Res., vol. 25, no. 8, pp. 1399-1407. 2017. https://doi.org/10.3727/096504016X14823648620870