Open Access

ARTICLE

Yangjing Chen, Shaoqiang Zhang, Ruimin Zhao, Qian Zhao, Ting Zhang
Oncology Research, Vol.25, No.8, pp. 1215-1222, 2017, DOI:10.3727/096504016X14791715355957
Abstract Medullary thyroid carcinoma (MTC) is a neuroendocrine cancer derived from parafollicular C cells in the thyroid gland. It has great interest as a research focus because of its unusual genetic, clinical, and prognostic characteristics. However, the pathogenesis in MTC is not completely clear. We investigated the role of miR- 9-3p and bladder cancer-associated protein (BLCAP) in MTC TT cells. First, miR-9-3p expression was upregulated in human MTC tissues and TT cells and compared to the control by RT-PCR. Flow cytometric analysis indicated that the cell cycle progression in TT cells was significantly inhibited by the… More >

View

152

Download

126

Open Access

ARTICLE

Valentina K. Todorova^*, Issam Makhoul^†, Ishwori Dhakal^‡, Jeanne Wei^§, Annjanette Stone^¶, Weleetka Carter^¶, Aaron Owen^*, V. Suzanne Klimberg^*
Oncology Research, Vol.25, No.8, pp. 1223-1229, 2017, DOI:10.3727/096504017X14876245096439
Abstract Doxorubicin (DOX) is a commonly used antineoplastic agent for the treatment of various malignancies, and its use is associated with unpredictable cardiotoxicity. Susceptibility to DOX cardiotoxicity is largely patient dependent, suggesting genetic predisposition. We have previously found that individual sensitivity to DOX cardiotoxicity was associated with differential expression of genes implicated in inflammatory response and immune trafficking, which was consistent with the increasing number of reports highlighting the important role of human leukocyte antigen (HLA) complex polymorphism in hypersensitivity to drug toxicity. This pilot study aimed to investigate DNA from patients treated with DOX-based chemotherapy… More >

View

181

Download

154

Open Access

ARTICLE

HaiYan Yang^*†, ZhiGang Peng^†, ZhenZhen Da^*, Xin Li^‡, YeXiao Cheng^*, BinBin Tan^§, Xin Xiang^¶, HaiPing Zheng^†, Yan Li^*, LanHua Chen^*, Ning Mo^†, XueXin Yan^†, Xiaolin Li^*, XiaoHua Hu^†
Oncology Research, Vol.25, No.8, pp. 1231-1243, 2017, DOI:10.3727/096504017X14850134190255
Abstract MicroRNAs (miRs) have been demonstrated to be involved in the development and progression of osteosarcoma (OS), but the molecular mechanism still remains to be fully investigated. The present study investigated the function of miR-148a in OS, as well as its underlying mechanism. Our data showed that miR-148a was significantly downregulated in OS tissues compared to their matched adjacent normal tissues, and also in OS cell lines compared to normal human osteoblast cells. Low expression of miR-148a was significantly associated with tumor progression and a poor prognosis for OS patients. Rho-associated coiled-coil kinase 1 (ROCK1) was… More >

View

153

Download

136

Open Access

ARTICLE

Makoto Akiyama^*†, Yoshihiro Sowa^*, Tomoyuki Taniguchi^*, Motoki Watanabe^*, Shingo Yogosawa^‡, Jo Kitawaki^†,Toshiyuki Sakai^*
Oncology Research, Vol.25, No.8, pp. 1245-1252, 2017, DOI:10.3727/096504017X14850164661097
Abstract Ovarian cancer is the most lethal disease among gynecological malignancies. More effective therapy is required to counter high recurrence rates and chemotherapy resistance. We investigated the efficacy and molecular mechanisms of three combined treatments (TCTs)—a novel histone deacetylase (HDAC) inhibitor OBP-801/YM753, 5-fluorouracil (5-FU), and paclitaxel (PTX)—in human ovarian cancer SKOV-3 and OVCAR-3 cells. The inhibition of cell growth was stronger with TCTs than with each single agent and with two combined treatments. The TCTs significantly induce G₂ phase arrest in both cell lines. We then analyzed the molecular mechanisms and found that the TCTs increased the More >

View

141

Download

98

Open Access

ARTICLE

Yuying Tan^*, Hanxin Yao^†, Jinghai Hu^‡, Lingyun Liu^§
Oncology Research, Vol.25, No.8, pp. 1253-1259, 2017, DOI:10.3727/096504017X14854310794561
Abstract Tripartite motif 44 (TRIM44), a member of the TRIM protein family, has been shown to play a role in tumor development and progression. However, the potential involvement of TRIM44 in prostate cancer has not been fully explored. Therefore, in the present study, we analyzed the expression of TRIM44 in prostate cancer and assessed the role of TRIM44 in the progression of prostate cancer. Our results showed that the expression of TRIM44 was significantly upregulated in human prostate cancer cell lines. In addition, knockdown of TRIM44 significantly inhibited the proliferation, migration, and invasion of prostate cancer More >

View

151

Download

106

Open Access

ARTICLE

Fang Chen^*1, Wei Xiong^*†1, Ke Dou^‡, Qing Ran^*
Oncology Research, Vol.25, No.8, pp. 1261-1267, 2017, DOI:10.3727/096504017X14871164924588
Abstract Forkhead box K1 (FOXK1) is a member of the FOX transcription factor family and plays an important role in the development of several tumors. However, the role of FOXK1 in the progression of prostate cancer remains unknown. Thus, the objectives of this study were to detect the expression of FOXK1 in prostate cancer and to examine its role in prostate cancer cells. We found that the expression of FOXK1 at both the mRNA and protein levels was significantly upregulated in human prostate cancer cell lines. In addition, the downregulation of FOXK1 obviously inhibited the cell… More >

View

146

Download

97

Open Access

ARTICLE

Hui Li^*, Zhigang Xiang^*, Yan Liu^*, Bin Xu^*, Jianzhou Tang^†
Oncology Research, Vol.25, No.8, pp. 1269-1282, 2017, DOI:10.3727/096504017X14850151453092
Abstract MicroRNAs (miRs), a class of small noncoding RNAs, are key gene regulators through inducing translational repression or degradation of their target genes. However, the regulatory mechanism of miR-133b underlying hepatocellular carcinoma (HCC) growth and metastasis remains largely unclear. Here we found that miR-133b was significantly downregulated in HCC tissues and cell lines. Moreover, low miR-133b levels were significantly associated with the malignant progression of HCC. LASP1, upregulated in HCC tissues and cell lines, was then identified as a novel target of miR-133b in HCC HepG2 and Hep3B cells. Moreover, the increased expression of LASP1 was… More >

View

147

Download

103

Open Access

ARTICLE

Maitham A. Khajah, Princy M. Mathew, Yunus A. Luqmani
Oncology Research, Vol.25, No.8, pp. 1283-1295, 2017, DOI:10.3727/096504017X14883245308282
Abstract Current mainstream pharmacological options for the treatment of endocrine-resistant breast cancer have limitations in terms of their side effect profile and lack of discrimination between normal and cancer cells. In the current study, we assessed the responses of normal breast epithelial cells MCF10A, estrogen receptorpositive (ER+ ) MCF-7, and ER-silenced pII breast cancer cells to inhibitors (either individually or in combination) of downstream signaling molecules. The expression/activity of ERK1/2, p38 MAPK, and Akt was determined by Western blotting. Cell proliferation, motility, and invasion were determined using MTT, wound healing, and Matrigel assays, respectively. Morphological changes… More >

View

163

Download

101

Open Access

ARTICLE

Jin Wang^*, Wenquan Pang^*, Zhenbai Zuo^*, Wenyan Zhang^*, Weidong He^†
Oncology Research, Vol.25, No.8, pp. 1297-1304, 2017, DOI:10.3727/096504017X14873430389189
Abstract Spinal osteosarcoma (OS) is a malignant tumor that has a poor outcome. MicroRNA-520b (miR-520b) acts as a cancer suppressor in various types of cancer. Because of the limited amount of literature on OS, we aimed to identify the role of miR-520b in OS. The miR-520b level in clinical spinal OS tissues and adjacent nontumor tissues as well as in cell lines was assessed. The effect of miR-520b on cell proliferation, migration, invasion, and frizzled-8 (FZD8) degradation were all evaluated. Alterations of key proteins involved in the Wnt/b-catenin pathway were assessed by Western blot analysis. In… More >

View

173

Download

97

Open Access

ARTICLE

Yingbo Xue, Tingting Ni, Ying Jiang, Yong Li
Oncology Research, Vol.25, No.8, pp. 1305-1316, 2017, DOI:10.3727/096504017X14850182723737
Abstract Growth arrest-specific transcript 5 (GAS5) has been demonstrated to correlate with clinicopathological characteristics and serve as a tumor suppressor in non-small cell lung cancer (NSCLC). However, the underlying mechanism of the competing endogenous RNA (ceRNA) regulatory network involving GAS5 in NSCLC remains to be elucidated. In this study, qRT-PCR results showed that GAS5 was downregulated and miR-135b was upregulated in NSCLC tissues and cells. The expressions of GAS5 and miR-135b changed inversely in response to irradiation. Gain-of-function experiments revealed that GAS5 overexpression and miR-135b downregulation significantly suppressed tumorigenesis by repressing cell proliferation and invasion, and More >

View

177

Download

110

Open Access

ARTICLE

Junfeng Zhang^*1, Kun Xu^†1, Lili Shi^*, Li Zhang^*, Zhaohua Zhao^*, Hao Xu^*, Fei Liang^*, Hongbo Li^*, Yan Zhao^*, Xi Xu^*, Yingfang Tian^‡
Oncology Research, Vol.25, No.8, pp. 1317-1327, 2017, DOI:10.3727/096504017X14874323871217
Abstract Increasing studies have suggested that microRNAs (miRNAs) are involved in the development of gliomas. MicroRNA-216a has been reported to be a tumor-associated miRNA in many types of cancer, either as an oncogene or as a tumor suppressor. However, little is known about the function of miR-216a in gliomas. The present study was designed to explore the potential role of miR-216a in gliomas. We found that miR-216a was significantly decreased in glioma tissues and cell lines. Overexpression of miR-216a significantly suppressed the proliferation, migration, and invasion of glioma cells. Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) More >

View

138

Download

102

Open Access

ARTICLE

Kairui Liu^*, Xiaolin Wu^*, Xian Zang^†, Zejian Huang^*, Zeyu Lin^‡, Wenliang Tan^*, Xiang Wu^*, Wenrou Hu^*, Baoqi Li^*, Lei Zhang^*
Oncology Research, Vol.25, No.8, pp. 1329-1340, 2017, DOI:10.3727/096504017X14876227286564
Abstract Overexpression of the tumor necrosis factor receptor-associated factor 4 (TRAF4) has been detected in many cancer types and is considered to foster tumor progression. However, the role of TRAF4 in hepatocellular carcinoma (HCC) remains elusive. In this study, we found that TRAF4 was highly expressed in HCC cell lines and HCC tissues compared with normal liver cell lines and adjacent noncancerous tissues. TRAF4 overexpression in HCC tissues was correlated with tumor quantity and vascular invasion. In vitro studies showed that TRAF4 was associated with HCC cell migration and invasion. An in vivo study verified that More >

View

157

Download

181

Open Access

ARTICLE

Chengyan Luo, Jiangnan Qiu
Oncology Research, Vol.25, No.8, pp. 1341-1348, 2017, DOI:10.3727/096504017X14867268787969
Abstract Cervical cancer is among the most common cancers inflicting women worldwide. Understanding the pathological mechanisms of cervical cancer development is critical for identifying novel targets for cervical cancer treatment. MicroRNAs (miRs) have various roles in regulating cancer development. In this study, we investigated the potential role of miR-181a and its target in regulating cervical cancer development and chemotherapy resistance. The expression of miR-181a was evaluated and modulated in several human cervical cancer cell lines. The role of miR-181a in regulating cervical cancer growth and chemotherapy sensitivity was investigated in cell culture models and mouse tumor… More >

View

148

Download

102

Open Access

ARTICLE

Li-Kun Yang^*1, Jie Zhu^*1, Yu-Hua Chen^†1, Dong-Liang Wang^‡, Hua Li^§, Liang-Jun Zhang^§, Jing-Ru Zhou^‡, Wei Liu^†
Oncology Research, Vol.25, No.8, pp. 1349-1355, 2017, DOI:10.3727/096504017X14874337324615
Abstract Angiopoietin-like protein 2 (ANGPTL2), a member of the glycoprotein family, is mainly secreted by adipose tissues under normal conditions. Recently, ANGPTL2 has been found to be upregulated in some types of cancers and is considered to be a tumor promoter. However, the functional significance of ANGPTL2 in glioma has not yet been elucidated. In this study, we investigated the specific role of ANGPTL2 in glioma. The results showed that ANGPTL2 was highly expressed in glioma tissues and cell lines. Knockdown of ANGPTL2 reduced the proliferative and invasive abilities of glioma cells. Moreover, the tumorigenesis assay More >

View

154

Download

102

Open Access

ARTICLE

Hua Tao, Yiqin Zhou, Chengyun Yao, Dayong Gu, Wei Chen, Jincheng Lu
Oncology Research, Vol.25, No.8, pp. 1357-1362, 2017, DOI:10.3727/096504017X14889842609577
Abstract The aim of this study was to evaluate the efficacy and toxicity of intensity-modulated radiotherapy concurrent with weekly docetaxel in patients with node-positive esophageal squamous cell carcinoma after radical surgery. Between January 2011 and December 2013, a total of 46 eligible patients were enrolled. All patients received intensity-modulated radiotherapy concurrent with weekly docetaxel (20 mg/m² ). Patients were treated 5 days per week at 2.0 Gy/day. The total dose of external radiotherapy given was 50 Gy in 25 fractions. The primary endpoints included treatment completion and safety. The secondary endpoint was to assess whether the approach… More >

View

212

Download

122

Open Access

ARTICLE

Shuke Ge^*, Dan Wang^†, Qinglong Kong^‡, Wei Gao^*, Jiayi Sun^*
Oncology Research, Vol.25, No.8, pp. 1363-1371, 2017, DOI:10.3727/096504017X14878536973557
Abstract miR-152, as a tumor suppressor, has been reported to be downregulated in a number of cancer cell lines and tumor tissues, including breast cancer. This study aimed to investigate the role of miR-152 in human breast cancer and its underlying mechanisms. Human breast cancer cell line HCC1806 was transfected with hsa-miR- 152-3p mimic, inhibitor, or scrambled negative controls. The efficiency of miR-152-3p transfection was evaluated by quantitative real-time PCR, and the effects on cell viability and apoptosis as well as on the PI3K/AKT signaling pathway were investigated by MTT assay, flow cytometry, and Western blot… More >

View

196

Download

123

Open Access

ARTICLE

Qingchun Li^*, Yuan Tian^†, Guangrui Hu^†, Yun Liang^†, Wei Bai^†, Hongjun Li^†
Oncology Research, Vol.25, No.8, pp. 1373-1382, 2017, DOI:10.3727/096504017X14878509668646
Abstract Long noncoding RNA (lncRNA) antisense noncoding RNA in the INK4 locus (ANRIL) is involved in several human cancers. However, the role of ANRIL in renal cell carcinoma (RCC) remains unclear. This study aimed to explore whether, and how, ANRIL affects the progression of RCC. First, the expression of ANRIL in clinical tumor tissues and four kinds of RCC cell lines was evaluated. After transfection, cell viability, colony number, apoptosis, migration, and invasion were assessed. The expression of proteins related to apoptosis, epithelial-tomesenchymal transition (EMT), and the β-catenin signaling pathway was then assessed. In addition, the… More >

View

134

Download

109

Open Access

ARTICLE

Xiaoyu Wu^*1, Jin Zhou^†1, Zhenfeng Wu^*, Che Chen^*, Jiayun Liu^*, Guannan Wu^*, Jing Zhai^*, Fukun Liu^*, Gang Li^‡
Oncology Research, Vol.25, No.8, pp. 1383-1390, 2017, DOI:10.3727/096504017X14879366402279
Abstract miR-101-3p has been identified as a tumor suppressor in several cancers, but its exact role in gastric adenocarcinoma is still largely unknown. In this study, we found that, compared with the RGM-1 human normal gastric epithelial cells, miR-101-3p was significantly downregulated in all six human gastric adenocarcinoma cell lines, including BGC-823, MNK-45, MGC-803, SGC-7901, AGS, and HGC-27. Overexpression of miR- 101-3p suppressed both the proliferation and invasion of AGS gastric adenocarcinoma cells, and knockdown of miR-101-3p displayed the opposite effect. In addition, miR-101-3p could directly target and suppress the expression of the serum response factor More >

View

152

Download

97

Open Access

ARTICLE

Ya-Li Gao^*1, Zi-Shen Zhao^†1, Ming-Yun Zhang^*, Li-Jie Han^*, Yu-Jin Dong^‡, Bo Xu^‡
Oncology Research, Vol.25, No.8, pp. 1391-1398, 2017, DOI:10.3727/096504017X14881559833562
Abstract Emerging evidence suggests that the long noncoding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) gene is involved in the pathogenesis of cervical cancer. However, the potential mechanism is rarely reported. Our study found that PVT1 was upregulated in cervical cancer tissue and cell lines. After transfecting PVT1 siRNA, the proliferation, migration, and invasion of cervical cancer cells were markedly decreased. miRNA expression profiles demonstrate that miR-424 was markedly downregulated in cervical cancer tissue. Bioinformatics analysis revealed that miR-424 was potentially targeted by PVT1, which was confirmed by dual-luciferase reporter assay. Pearson’s correlation analysis showed that More >

View

177

Download

108

Open Access

ARTICLE

Zhuang Kun^*†, Guo Hanqing^*, Tang Hailing^*, Yan Yuan^*, Zhang Jun^†, Zhang Lingxia^*, Han Kun^*, Zhang Xin^*
Oncology Research, Vol.25, No.8, pp. 1399-1407, 2017, DOI:10.3727/096504016X14823648620870
Abstract Gastric cancer (GC) is one of the most frequent epithelial malignancies worldwide. The gastrointestinal (GI) peptide gastrin is an important regulator of the secretion and release of gastric acid from stomach parietal cells, and it also plays a vital role in the development and progression of GC. The aim of the current study was to investigate the role and underlying mechanism of gastrin and autophagy in regulating GC tumorigenesis. Gastrin-17 amide (G-17) was applied in the GC cell lines SGC7901 and MGC-803. The results showed that G-17 maintained the high viability of SGC7901 and MGC-803.… More >

View

155

Download

110

Open Access

ARTICLE

Rui Jiang^*, Chao Zhang^†, Guangyao Liu^*, Rui Gu^*, Han Wu^*
Oncology Research, Vol.25, No.8, pp. 1409-1419, 2017, DOI:10.3727/096504017X14882829077237
Abstract Osteosarcoma is the most common primary bone malignancy manifested predominantly in children and young adults. Studies indicate that miR-107 is involved in the pathogenesis of osteosarcoma and that tropomyosin 1 (TPM1) acts as a tumor suppressor in many types of cancer. In this study, we analyzed the effect of miR-107 on human osteosarcoma cells and investigated the mechanism in which TPM1 is involved. miR-107 expression in human osteosarcoma tissues and cells was analyzed in quantitative real-time PCR (qRT-PCR). Human osteosarcoma (U2OS) cells were transfected with miR-107 mimic, inhibitor, or scramble controls to evaluate the effect… More >

View

240

Download

100