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Hai-tao Geng^*, Rui-juan Cao^*, Lei Cheng^†, Chun-yuan Liu^†
Oncology Research, Vol.25, No.7, pp. 1039-1046, 2017, DOI:10.3727/096504016X14813914187138
Abstract Hepatocyte cell adhesion molecule (hepaCAM), a new type of CAM, belongs to the immunoglobulin superfamily. Recently, hepaCAM was reported to be implicated in cancer development, and many researchers investigated its biological function in the tumorigenesis of various cancers. However, what kind of role hepaCAM plays in colorectal cancer (CRC) remains unknown. In this study, we found that hepaCAM was downregulated in CRC tissues and cell lines. Overexpression of hepaCAM inhibited CRC cell proliferation, migration, and invasion in vitro. Furthermore, the tumorigenesis assay showed that increased expression of hepaCAM suppressed CRC tumor growth and metastasis in More >

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Paweena Dana^*†‡, Ryusho Kariya^‡, KulthidaVaeteewoottacharn^*†, Kanlayanee Sawanyawisuth^*†, Wunchana Seubwai^†§, Kouki Matsuda^‡, Seiji Okada^‡, Sopit Wongkham^*†
Oncology Research, Vol.25, No.7, pp. 1047-1059, 2017, DOI:10.3727/096504016X14813899000565
Abstract CD147 is a transmembrane protein that can induce the expression and activity of matrix metalloproteinases (MMPs). Expression of CD147 has been shown to potentiate cell migration, invasion, and metastasis of cancer. In this study, the critical role of CD147 in metastasis was elucidated using CD147-overexpressing cholangiocarcinoma (CCA) cells in vitro and in vivo. The molecular mechanism, demonstrated herein, supported the hypothesis that metastasis increased in CD147-overexpressing cells. Five CD147-overexpressing clones (Ex-CD147) were established from a low CD147-expressing CCA cell line, KKU-055, using lentivirus containing pReceiver-Lenti-CD147. The metastatic capability was determined using the tail vein injection… More >

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Huijie Fan^*1, Jing Li^*1, Yongxu Jia^*, Jingjing Wu^*, Long Yuan^†, Mingjun Li^*, Jiangqi Wei^‡, Benling Xu^§
Oncology Research, Vol.25, No.7, pp. 1061-1068, 2017, DOI:10.3727/096504016X14830466773541
Abstract Ribosomal protein L34 (RPL34) belongs to the L34E family of ribosomal proteins and contains a zinc finger motif. Aberrant expression of RPL34 has been reported in several human malignancies. However, the precise role and potential underlying mechanisms of RPL34 in human esophageal cancer remain largely unknown. Thus, the objective of this study was to investigate the role of RPL34 in esophageal cancer progression. Our results showed that the expression of RPL34 at both the mRNA and protein levels was frequently upregulated in esophageal cancer cell lines. Knockdown of RPL34 efficiently inhibited esophageal cancer cell proliferation, More >

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Yueli Gu^*, Jinchun Si^†, Xichun Xiao^*, Ying Tian^*, Shuo Yang^*
Oncology Research, Vol.25, No.7, pp. 1069-1079, 2017, DOI:10.3727/096504016X14829256525028
Abstract Aberrant expression of microRNA-92a (miR-92a) has been investigated in various cancers. However, the function and mechanism of miR-92a in acute myeloid leukemia (AML) remain to be elucidated. Our data showed that miR-92a was evidently downregulated and methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) was remarkably upregulated in AML cell lines HL-60 and THP-1. Dual luciferase reporter assay revealed that MTHFD2 was a direct target of miR-92a. Gain- and loss-of-function analysis demonstrated that MTHFD2 knockdown or miR-92a overexpression notably inhibited proliferation and promoted apoptosis of AML cell lines. Restoration of MTHFD2 expression reversed proliferation inhibition and apoptosis induction of More >

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Zhangjie Jiang^*1, Yida Zhang^†1, Runfu Cao^†, Li Li^‡, Kezhao Zhong^†, Qingsheng Chen^†, Jianjun Xiao^†
Oncology Research, Vol.25, No.7, pp. 1081-1087, 2017, DOI:10.3727/096504016X14831120463349
Abstract miRNAs play a key role in the carcinogenesis of many cancers, including bladder cancer. In the current study, the role of miR-5195-3p, a quite recently discovered and poorly studied miRNA, in the proliferation and invasion of human bladder cancer cells was investigated. Our data displayed that, compared with healthy volunteers (control) and SU-HUC-1 normal human bladder epithelial cells, miR-5195-3p was sharply downregulated in bladder cancer patients and five human bladder cancer cell lines. The oligo miR-5195-3p mimic or miR-5195-3p antagomir was subsequently transfected into both T24 and BIU-87 bladder cancer cell lines. The miR-5195-3p mimic… More >

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Lei Yang^*, Fei Xie^†, Shuangqing Li^‡
Oncology Research, Vol.25, No.7, pp. 1089-1095, 2017, DOI:10.3727/096504016X14784668796788
Abstract Homeobox B7 (HOXB7), a member of the HOX gene family, plays a role in tumorigenesis. However, until now the expression status and role of HOXB7 in osteosarcoma remain unclear. Therefore, the present study aimed to investigate the functional role and mechanism of HOXB7 in osteosarcoma. Our results demonstrated that HOXB7 was overexpressed in osteosarcoma cell lines. Downregulation of HOXB7 significantly inhibited osteosarcoma cell proliferation in vitro, as well as attenuated xenograft tumor growth in vivo. Downregulation of HOXB7 also inhibited the migration and invasion of osteosarcoma cells. Furthermore, downregulation of HOXB7 significantly suppressed the protein More >

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Yidong Cao^*1, Liang Zhang^*1, Minghao Wei^*, Xue Jiang^†, Dong Jia^*
Oncology Research, Vol.25, No.7, pp. 1097-1107, 2017, DOI:10.3727/096504017X14836170586829
Abstract Emerging evidence has suggested that aberrantly expressed microRNAs (miRNAs) are associated with glioma development and progression. The aberrant expression of miR-409-3p has been reported in several human cancers. However, little is known about the function of miR-409-3p in gliomas. The aim of this study was to investigate the specific role and molecular mechanism of miR-409-3p in gliomas. In the present study, we found that miR-409-3p was downregulated in glioma tissue and cell lines. Overexpression of miR-409-3p inhibited glioma cell invasion and proliferation, whereas suppression of miR-409-3p promoted glioma cell invasion and proliferation. High-mobility group nucleosome-binding More >

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Yan Zhen^*1, Jia Liu^*†1, Yujie Huang^*†1, Yajun Wang^*, Wen Li^*†, Jun Wu^*†
Oncology Research, Vol.25, No.7, pp. 1109-1116, 2017, DOI:10.3727/096504016X14800889609439
Abstract Although increasing evidence indicates that the deregulation of microRNAs (miRNAs) contributes to tumorigenesis and invasion, little is known about the role of miR-133b in human non-small cell lung cancer (NSCLC). In the present study, we revealed that the introduction of miR-133b dramatically suppressed NSCLC cell growth, migration, and invasion in vitro. On the contrary, miR-133b inhibitors promoted cell growth, migration, and invasion in vitro. Further studies revealed that matrix metallopeptidase 9 (MMP9) is a direct target gene of miR-133b. Silencing MMP9 inhibited cell growth, migration, and invasion of NSCLC cells, which was consistent with the More >

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Tinghui Jiang^*, Mengfan Li^†, Qiuyin Li^‡, Zhiqiang Guo^§, Xianjun Sun^‡, Xufeng Zhang^§, Yan Liu^‡, Wenyi Yao^‡, Ping Xiao^*
Oncology Research, Vol.25, No.7, pp. 1117-1127, 2017, DOI:10.3727/096504016X14821952695683
Abstract Some microRNAs (miRs) have been demonstrated to play promoting or tumor-suppressing roles in the development and progression of hepatocellular carcinoma (HCC). However, the regulatory mechanism of miR-98-5p in HCC still remains largely unclear. In the present study, our data showed that miR-98-5p was significantly downregulated in 84 cases of HCC tissues compared to the matched adjacent nontumor tissues. In addition, downregulation of miR-98-5p was associated with tumor size, portal vein tumor embolus, node metastasis, and clinical stage in HCC. HCC patients with low expression of miR-98-5p showed a shorter survival time compared with those with… More >

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Antonello Di Paolo¹, Paola Orlandi¹, Teresa Di Desidero, Romano Danesi, Guido Bocci
Oncology Research, Vol.25, No.7, pp. 1129-1140, 2017, DOI:10.3727/096504017X14841698396900
Abstract The combination of folinate salts to 5-fluoruracil (5-FU)-based schedules is an established clinical routine in the landscape of colorectal cancer treatment. The aim of this study was to investigate the pharmacological differences between the sequential administration of folinate salts (1 h before, as in clinical routine) followed by 5-FU and the simultaneous administration of both drugs. Proliferation and apoptotic assays were performed on human colon cancer cells exposed to 5-FU, calcium (CaLV), or disodium (NaLV) levofolinate or their simultaneous and sequential combination for 24 and 72 h. TYMS and SLC19A1 gene expression was performed with… More >

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Huan Shen^*1, Li Liu^†1, Yongjin Yang^*, Wenxing Xun^‡, Kewen Wei^‡, Guang Zeng^‡
Oncology Research, Vol.25, No.7, pp. 1141-1152, 2017, DOI:10.3727/096504017X14841698396784
Abstract Oral squamous cell carcinoma (OSCC) is a common cancer of the head and neck. Betulinic acid (BA) is a naturally occurring pentacyclic triterpenoid. The present study was designed to explore the effects of BA on OSCC KB cell proliferation in vitro and on implanted tumor growth in vivo and to examine the possible molecular mechanisms. The results showed that BA dose-dependently inhibited KB cell proliferation and decreased implanted tumor volume. In addition, BA significantly promoted mitochondrial apoptosis, as reflected by an increase in TUNEL+ cells and the activities of caspases 3 and 9, an increase… More >

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Ning Wu^*, Changyu He^†, Bohui Zhu^*, Jinling Jiang^†, Yiwen Chen^*, Tao Ma^†
Oncology Research, Vol.25, No.7, pp. 1153-1159, 2017, DOI:10.3727/096504017X14845839228545
Abstract Gastric cancer (GC) is one of the most common cancers and the second leading cause of cancer deaths in the world. Many factors have been reported regarding the progression and development of GC. In this study, we aimed to investigate the correlation of 3-phosphoinositide dependent protein kinase-1 (PDK-1) with cell viability, migration, and invasion of GC. The expression of PDK-1 was measured in different GC cell lines. Thereafter, the expression of PDK-1 was interfered by small hairpin RNA (shRNA) and then incubated with or without the inhibitor of nuclear factor-kB (NF-kB) pyrrolidine dithiocarbamate (PDTC). We… More >

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Bin Xu, Hehuan Xia, Junming Cao, Zhihong Wang, Yipeng Yang, Yongsheng Lin
Oncology Research, Vol.25, No.7, pp. 1161-1168, 2017, DOI:10.3727/096504017X14841698396829
Abstract Currently, multiple microRNAs (miRNAs) have been found to play vital roles in the pathogenesis of osteosarcoma. This study aimed to investigate the role of miR-21 in osteosarcoma. The level of miR-21 in 20 pairs of osteosarcoma and corresponding adjacent tissues was monitored by qPCR. Human osteosarcoma cell line SAOS-2 was transfected with either miR-21 mimic or miR-21 inhibitor, and then cell viability, survival, and apoptosis were measured by MTT, colony formation assay, and flow cytometry. A target of miR-21 was predicted by the microRNA.org database and verified in vitro by using luciferase reporter, qPCR, and More >

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Chang Li^*, Xiaoping Li^†, Shuohui Gao^*, Chang Li^‡, Lianjun Ma^§
Oncology Research, Vol.25, No.7, pp. 1169-1176, 2017, DOI:10.3727/096504017X14847395834985
Abstract Gastric cancer is the fourth most common type of cancer and the second highest leading cause of cancer-related deaths worldwide. It has already been established that miR-133a is involved in gastric cancer. In this study, we investigated the molecular mechanisms by which miR-133a inhibits the proliferation of gastric cancer cells. We analyzed the proliferative capacity of human gastric cancer cells SNU-1 using an MTT assay. Cell apoptosis was determined using flow cytometry. The expression levels of ERBB2, p-ERK1/2, and p-AKT in SNU-1 cells were determined using Western blot analysis. To confirm that ERBB2 is a… More >

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Sukru Gulluoglu^*†, Mesut Sahin^‡, Emre Can Tuysuz^†, Cumhur Kaan Yaltirik^§, Aysegul Kuskucu^*, Ferda Ozkan^¶, Fikrettin Sahin^†, Ugur Ture^§, Omer Faruk Bayrak^*
Oncology Research, Vol.25, No.7, pp. 1177-1188, 2017, DOI:10.3727/096504017X14874349473815
Abstract Chordomas are rare tumors of the spine and skull base that are locally destructive and resistant to chemotherapy and radiation therapy, with a poor prognosis and limited therapeutic options. Chordoma patients have a long life expectancy with high mortality from the disease. Cancer stem cells, which are known to exist in chordomas, have extensive proliferative and self-renewal potential and are responsible for maintaining tumor heterogeneity along with chemotherapy and radiotherapy resistance. Leukemia inhibitory factor (LIF) has multiple functions in stem cell biology, the immune response, and cancer, and is potentially a key molecule that allows… More >

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Jie Guo^*†, Jinqi Ma^‡, Guosheng Zhao^‡, Guocai Li^‡, Yunfeng Fu^‡, Yanwei Luo^‡, Rong Gui^‡
Oncology Research, Vol.25, No.7, pp. 1189-1197, 2017, DOI:10.3727/096504017X14865126670075
Abstract Nasopharyngeal carcinoma (NPC) is a distinct head and neck cancer, which is occurring at a high frequency in Southern China. Emerging studies have shown that long noncoding RNAs (lncRNAs) play a critical role in carcinogenesis and progression. In this study, we established a comprehensive lncRNA profile in NPC and found that 35 lncRNAs were differentially expressed in NPC. We found that LINC0086 was decreased in NPC patient serum samples and tissues. The Kaplan–Meier survival curve showed that patients with high LINC0086 expression had a higher survival rate than those with low LINC0086 expression. LINC0086 expression… More >

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Peihe Wang^*, Yuanyuan Cai^*, Dongju Lin^†, Yingxiao Jiang^*
Oncology Research, Vol.25, No.7, pp. 1199-1205, 2017, DOI:10.3727/096504017X14873444858101
Abstract Gamma ray can promote cancer cell apoptosis and cell cycle arrest. It is often used in the clinical treatment of tumors, including lung cancer. In this study, we aimed to explore the role of gamma ray treatment and its correlation with BTG2 in cell proliferation, apoptosis, and cell cycle arrest regulation in a lung cancer cell line. A549 cell viability, apoptosis rate, and cell cycle were investigated after gamma ray treatment. We then used siRNA for BTG2 to detect the effect of BTG2 knockdown on the progress of gamma ray-treated lung cancer cells. Finally, we… More >

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Yi-Bin Meng, Xin He, Yun-Fei Huang, Qi-Ning Wu, Yong-Cun Zhou, Ding-Jun Hao
Oncology Research, Vol.25, No.7, pp. 1207-1214, 2017, DOI:10.3727/096504017X14886679715637
Abstract It has been determined that long noncoding RNAs (lncRNAs) are identified as a potential regulatory factor in multiple tumors as well as multiple myeloma (MM). However, the role of colorectal neoplasia differentially expressed (CRNDE) in the pathogenesis of MM remains unclear. In this study, we found that the CRNDE expression level, in MM samples and cell lines, is higher than that in the control detected by real-time qPCR, which is also closely related to tumor progression and poor survival in MM patients. Knockdown of CRNDE significantly inhibits the proliferative vitality of MM cells (U266 and… More >

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