Open Access

ARTICLE

miR-326 Inhibits Gastric Cancer Cell Growth Through Downregulating NOB1

Sheqing Ji, Bin Zhang, Ye Kong, Fei Ma, Yawei Hua

Department of General Surgery, Henan Cancer Hospital, Zhengzhou University Cancer Hospital, Zhengzhou, Henan, P.R. China

Oncology Research 2017, 25(6), 853-861. https://doi.org/10.3727/096504016X14759582767486

Abstract

MicroRNAs (miRNAs) play a crucial role in the development and progression of human cancers, including gastric cancer (GC). The discovery of miRNAs may provide a new and powerful tool for studying the mechanism, diagnosis, and treatment of GC. In this study, we aimed to investigate the role of miR-326 in the development and progression of GC. Quantitative PCR (qPCR) was used to measure the expression level of miR-326 in GC tissues and cell lines. We found that miR-326 was significantly downregulated during GC. In addition, overexpression of miR-326 inhibited GC cell proliferation. Fluorescence-activated cell sorting (FACS) further showed that miR-326 significantly induced GC cell G₂/M arrest. Subsequent dual-luciferase reporter assay identified one of the proto-oncogene NOB1 as a direct target of miR-326, and NOB1 can save growth inhibition caused by miR-326. We also confirmed that the growth inhibition caused by miR-326 is associated with AKT pathway activation. Taken together, our results indicate that miR-326 could serve as a potential diagnostic biomarker and therapeutic option for GC in the near future.

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Keywords

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