Open Access
ARTICLE
Downregulation of MicroRNA-449 Promotes Migration and Invasion of Breast Cancer Cells by Targeting Tumor Protein D52 (TPD52)
Zhiling Zhang, Jiawei Wang, Runfang Gao, Xuan Yang, Yafen Zhang, Jie Li, Jing Zhang, Xingjuan Zhao, Chunfang Xi, Xiaoting Lu
Department of Breast Surgery, Shanxi Provincial People’s Hospital, Taiyuan, P.R. China
Oncology Research 2017, 25(5), 753-761. https://doi.org/10.3727/096504016X14772342320617
14 June 2024 Editors Note:
Readers are alerted that concerns were raised about the integrity of some of the data presented in the article. Further editorial action will be taken once this matter has been resolved.
Abstract
Our study aimed to investigate whether microRNA-449 (miR-449) plays a key role in regulating the migration
and invasion of breast cancer cells via targeting tumor protein D52 (TPD52). The results of the qRT-PCR and
Western blotting showed that, in comparison with normal breast tissues and cells, miR-449 was significantly
downregulated in breast cancer tissues and cells, while TPD52 was markedly upregulated. After transfection
with an miR-449 inhibitor, suppression of miR-449 significantly promoted cell migration and invasion. Also,
when miR-449 was overexpressed by transfection with miR-449 mimics, E-cadherin expression significantly
increased, and the expression of N-cadherin and vimentin were markedly decreased, whereas the opposite
effects were obtained when miR-449 was suppressed by transfection with an miR-449 inhibitor. TPD52 was
also confirmed as the direct target of miR-449 via luciferase reporter analysis. Knockdown of TPD52 significantly alleviated the effects of miR-449 overexpression on cell migration and invasion, as well as the
expression of E-cadherin, N-cadherin, and vimentin. Our results indicate that downregulation of miR-449
may promote the migration and invasion of breast cancer cells by targeting TPD52. miR-449 may serve as a
potential target in the therapy of breast cancer.
Keywords
Cite This Article
APA Style
Zhang, Z., Wang, J., Gao, R., Yang, X., Zhang, Y. et al. (2017). Downregulation of microrna-449 promotes migration and invasion of breast cancer cells by targeting tumor protein D52 (TPD52). Oncology Research, 25(5), 753-761. https://doi.org/10.3727/096504016X14772342320617
Vancouver Style
Zhang Z, Wang J, Gao R, Yang X, Zhang Y, Li J, et al. Downregulation of microrna-449 promotes migration and invasion of breast cancer cells by targeting tumor protein D52 (TPD52). Oncol Res. 2017;25(5):753-761 https://doi.org/10.3727/096504016X14772342320617
IEEE Style
Z. Zhang et al., "Downregulation of MicroRNA-449 Promotes Migration and Invasion of Breast Cancer Cells by Targeting Tumor Protein D52 (TPD52)," Oncol. Res., vol. 25, no. 5, pp. 753-761. 2017. https://doi.org/10.3727/096504016X14772342320617