Open Access
ARTICLE
MicroRNA-219-5p Represses the Proliferation, Migration, and Invasion of Gastric Cancer Cells by Targeting the LRH-1/Wnt/β-Catenin Signaling Pathway
Chunsheng Li*, Jingrong Dong†, Zhenqi Han†, Kai Zhang‡
* Department of Gastrointestinal Colorectal and Anal Surgery, China–Japan Union Hospital of Jilin University,
Changchun, Jilin, P.R. China
† Endoscopic Center, The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, Jilin, P.R. China
‡ Department of Colorectal and Anal Surgery, The Second Hospital of Jilin University, Changchun, Jilin, P.R. China
Oncology Research 2017, 25(4), 617-627. https://doi.org/10.3727/096504016X14768374457986
Abstract
MicroRNAs (miRNAs) are reportedly involved in gastric cancer development and progression. In particular,
miR-219-5p has been reported to be a tumor-associated miRNA in human cancer. However, the role of miR-
219-5p in gastric cancer remains unclear. In this study, we investigated for the first time the potential role and
underlying mechanism of miR-219-5p in the proliferation, migration, and invasion of human gastric cancer
cells. miR-219-5p was found to be markedly decreased in gastric cancer tissues and cell lines compared with
adjacent tissues and normal gastric epithelial cells. miR-219-5p mimics or anti-miR-219-5p was transfected
into gastric cancer cell lines to overexpress or suppress miR-219-5p expression, respectively. Results showed
that miR-219-5p overexpression significantly decreased the proliferation, migration, and invasion of gastric
cancer cells. Conversely, miR-219-5p suppression demonstrated a completely opposite effect. Bioinformatics
and luciferase reporter assays indicated that miR-219-5p targeted the 3'-untranslated region of the liver receptor homolog-1 (LRH-1), a well-characterized oncogene. Furthermore, miR-219-5p inhibited the mRNA and
protein levels of LRH-1. LRH-1 mRNA expression was inversely correlated with miR-219-5p expression
in gastric cancer tissues. miR-219-5p overexpression significantly decreased the Wnt/β-catenin signaling
pathway in gastric cancer cells. Additionally, LRH-1 restoration can markedly reverse miR-219-5p-mediated
tumor suppressive effects. Our study suggests that miR-219-5p regulated the proliferation, migration, and
invasion of human gastric cancer cells by suppressing LRH-1. miR-219-5p may be a potential target for
gastric cancer therapy.
Keywords
Cite This Article
APA Style
Li, C., Dong, J., Han, Z., Zhang, K. (2017). Microrna-219-5p represses the proliferation, migration, and invasion of gastric cancer cells by targeting the lrh-1/wnt/β-catenin signaling pathway. Oncology Research, 25(4), 617-627. https://doi.org/10.3727/096504016X14768374457986
Vancouver Style
Li C, Dong J, Han Z, Zhang K. Microrna-219-5p represses the proliferation, migration, and invasion of gastric cancer cells by targeting the lrh-1/wnt/β-catenin signaling pathway. Oncol Res. 2017;25(4):617-627 https://doi.org/10.3727/096504016X14768374457986
IEEE Style
C. Li, J. Dong, Z. Han, and K. Zhang "MicroRNA-219-5p Represses the Proliferation, Migration, and Invasion of Gastric Cancer Cells by Targeting the LRH-1/Wnt/β-Catenin Signaling Pathway," Oncol. Res., vol. 25, no. 4, pp. 617-627. 2017. https://doi.org/10.3727/096504016X14768374457986