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ARTICLE
Truncated Bid Overexpression Induced by Recombinant Adenovirus Cre/LoxP System Suppresses the Tumorigenic Potential of CD133+ Ovarian Cancer Stem Cells
Qifang Long*, Weipei Zhu*, Jundong Zhou†, Jinchang Wu†, Weixian Lu‡, Cui Zheng‡, Dongmei Zhou‡, Ling Yu‡, Ru Yang‡
* Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Soochow University,
Suzhou, Jiangsu Province, P.R. China
† Department of Radio-Oncology, Nanjing Medical University Affiliated Suzhou Hospital,
Suzhou, Jiangsu Province, P.R. China
‡ Department of Gynecology and Obstetrics, Nanjing Medical University Affiliated Suzhou Hospital,
Suzhou, Jiangsu Province, P.R. China
Oncology Research 2017, 25(4), 595-603. https://doi.org/10.3727/096504016X14765492198706
Abstract
Ovarian cancer is one of the most lethal malignant gynecologic tumors with a high relapse rate worldwide.
Cancer stem cells (CSCs) have been identified in ovarian cancer and other malignant tumors as a small
population of cells that are capable of self-renewal and multidifferentiation. CD133
+
ovarian CSCs have been
reported to be more tumorigenic and more resistant to chemotherapeutic treatment. Thus, CD133 has emerged
as one of the most promising therapeutic markers for ovarian cancer treatment. In the current study, we constructed a recombinant adenovirus Cre/loxP regulation system to selectively introduce truncated Bid (tBid)
expression specifically targeting CD133
+
in ovarian CSCs. The results demonstrated that the coinfection
of Ad-CD133-Cre and Ad-CMV-LoxP-Neo-LoxP-tBid significantly increased tBid expression in CD133
+
ovarian CSCs. Moreover, the tBid overexpression induced by a recombinant adenovirus Cre/loxP system
dramatically inhibited cell proliferation and invasion, significantly elevated cell apoptosis, and activated the
mitochondrial apoptosis pathway in CD133
+
ovarian CSCs. Additionally, recombinant adenovirus Cre/loxP
system-mediated tBid overexpression suppressed the tumorigenic potential of CD133
+
ovarian CSCs in a
xenograft mouse model. In conclusion, our study successfully constructed a recombinant adenovirus Cre/loxP
system and induced tBid overexpression in CD133
+
ovarian CSCs, providing a new therapeutic approach for
ovarian cancer treatment.
Keywords
Cite This Article
APA Style
Long, Q., Zhu, W., Zhou, J., Wu, J., Lu, W. et al. (2017). Truncated bid overexpression induced by recombinant adenovirus cre/loxp system suppresses the tumorigenic potential of cd133+ ovarian cancer stem cells. Oncology Research, 25(4), 595-603. https://doi.org/10.3727/096504016X14765492198706
Vancouver Style
Long Q, Zhu W, Zhou J, Wu J, Lu W, Zheng C, et al. Truncated bid overexpression induced by recombinant adenovirus cre/loxp system suppresses the tumorigenic potential of cd133+ ovarian cancer stem cells. Oncol Res. 2017;25(4):595-603 https://doi.org/10.3727/096504016X14765492198706
IEEE Style
Q. Long et al., "Truncated Bid Overexpression Induced by Recombinant Adenovirus Cre/LoxP System Suppresses the Tumorigenic Potential of CD133+ Ovarian Cancer Stem Cells," Oncol. Res., vol. 25, no. 4, pp. 595-603. 2017. https://doi.org/10.3727/096504016X14765492198706