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Juntao Yao^*†, Xuan Yao^‡, Tao Tian^*, Xiao Fu^*, Wenjuan Wang^*, Suoni Li^§, Tingting Shi^*, Aili Suo^*, Zhiping Ruan^*, Hui Guo^*, Kejun Nan^*, Xiongwei Huo^¶
Oncology Research, Vol.25, No.3, pp. 305-316, 2017, DOI:10.3727/096504016X14734149559061
Abstract ABCB5 belongs to the ATP-binding cassette (ABC) superfamily, which is recognized for playing a role in the failure of chemotherapy. ABCB5 has also been found to be overexpressed at the transcriptional level in a number of cancer subtypes, including breast cancer. However, the exact mechanism ABCB5 uses on cancer cell metastasis is still unclear. In the present study, we demonstrate that ABCB5 expression was increased in metastatic tissues when compared with nonmetastatic tissues. ABCB5 can significantly enhance metastasis and epithelial–mesenchymal transition (EMT), while knockdown of ABCB5 inhibited these processes. Microarray analysis indicated that ZEB1 may More >

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Shuye Lin^*†, Bonan Lin^*, Xiaoyue Wang^*, Yuanming Pan^‡, Qing Xu^*, Jin-Shen He^*, Wanghua Gong^§, Rui Xing^‡, Yuqi He^¶, Lihua Guo^*, Youyong Lu^‡, Ji Ming Wang^†, Jiaqiang Huang^*†
Oncology Research, Vol.25, No.3, pp. 317-329, 2017, DOI:10.3727/096504016X14734735156265
Abstract The ATPase H⁺/K⁺ Transporting Beta Subunit (ATP4B) encodes the b subunit of the gastric H⁺, K⁺ -ATPase, which controls gastric acid secretion and is therefore a target for acid reduction. Downregulation of ATP4B was recently observed in human gastric cancer (GC) without known mechanisms. In the present study, we demonstrated that ATP4B expression was decreased in human GC tissues and cell lines associated with DNA hypermethylation and histone hypoacetylation of histone H3 lysine 9 at its intragenic region close to the transcriptional start site. The expression of ATP4B was restored in GC cell lines by treatment with… More >

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Bingqian Ding¹, Bei Cui¹, Ming Gao, Zhenjiang Li, Chenyang Xu, Shaokang Fan, Weiya He
Oncology Research, Vol.25, No.3, pp. 331-340, 2017, DOI:10.3727/096504016X14736286083065
Abstract Ras-related protein 25 (Rab25) is a member of the Rab family, and it has been reported to play an important role in tumorigenesis. However, its direct involvement in human glioblastoma multiforme (GBM) is still unclear. The aim of the current study was to investigate the potential role of Rab25 in the growth, proliferation, invasion, and migration of human GBM. Our results showed that Rab25 expression was significantly higher in human GBM cell lines compared with a normal astrocyte cell line. In vitro functional studies revealed that knockdown of Rab25 reduced cell proliferation and inhibited invasion More >

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Vivian Adamski^*, Anne Dorothée Schmitt^*, Charlotte Flüh^*, Michael Synowitz^*, Kirsten Hattermann^†1, Janka Held-Feindt^*1
Oncology Research, Vol.25, No.3, pp. 341-353, 2017, DOI:10.3727/096504016X14737243054982
Abstract Gliomas are the most common primary brain tumors. The most malignant form, the glioblastoma multiforme (GBM; WHO IV), is characterized by an invasive phenotype, which enables the tumor cells to infiltrate into adjacent brain tissue. When investigating GBM migration and invasion properties in vitro, in most cases GBM cell lines were analyzed. Comprehensive investigations focusing on progression-dependent characteristics of migration processes using fresh human glioma samples of different malignancy grades do not exist. Thus, we isolated fast-migrating tumor cells from fresh human glioma samples of different malignancy grades (astrocytomas WHO grade II, grade III, GBM,… More >

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Guojun Wang, Yang Fu, Guanghui Liu, Yanwei Ye, Xiefu Zhang
Oncology Research, Vol.25, No.3, pp. 355-364, 2017, DOI:10.3727/096504016X14738114257367
Abstract MicroRNAs (miRNAs) play an important role in carcinogenesis. miR-218 is one of the most known miRNAs and has been demonstrated to inhibit progression in gastric cancer. However, the underlying molecular mechanism is not established. In this study, qRT-PCR and Western blot indicated that miR-218 was downregulated in gastric cancer cell lines SGC7901 and BGC823 compared to that in normal gastric epithelial cell line GES-1. MTT and wound scratch assays suggested that overexpression of miR-218 markedly suppressed cell proliferation, migration, and EMT of gastric cancer cells. Furthermore, we proved that WASF3 was a direct target of More >

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Zhenjiang Li^*1, Chenyang Xu^*1, Ming Gao^*, Bingqian Ding^*, Xinting Wei^†, Nan Ji^‡
Oncology Research, Vol.25, No.3, pp. 365-372, 2017, DOI:10.3727/096504016X14738135889976
Abstract Jumonji AT-rich interactive domain 2 (JARID2) is a member of the Jumonji family of proteins and has been proposed as an oncogene in several types of human cancer. However, the role of JARID2 in human glioma has not yet been understood. The present study was designed to determine the roles of JARID2 in the proliferation and migration in human glioma cells and the growth of glioma cells in nude mice. Our data indicate that JARID2 is upregulated in human glioma tissues and cell lines. Knockdown of JARID2 obviously inhibits the proliferation of U87MG cells and More >

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Zhao Xiaoguang^*1,2, Liu Meirong^†1, Zhang Jingjing^‡, Zhang Ruishen^§, Zhang Qing^¶, Tan Xiaofeng^†2
Oncology Research, Vol.25, No.3, pp. 373-380, 2017, DOI:10.3727/096504016X14741486659473
Abstract The long noncoding CPS1 intronic transcript 1 (lncRNA CPS1-IT1) is a recently identified tumor suppressor in the lncRNA family of proteins. Whether this lncRNA plays any functional role in solid tumors remains largely unknown. The present study aimed to investigate the role of lncRNA CPS1-IT1 in human lung cancer. Expression of lncRNA CPS1-IT1 was initially assessed in human lung cancer and in a series of lung cancer cell lines. The effects of CPS1-IT1 overexpression on cell proliferation, migration, and invasion were examined in lung cancer cell lines A549 and 95D. It was found that lncRNA… More >

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Gulijiahan Aierken¹, Ayinuer Seyiti¹, Mayinuer Alifu, Gulina Kuerban
Oncology Research, Vol.25, No.3, pp. 381-388, 2017, DOI:10.3727/096504016X14741511303522
Abstract The tripartite motif (TRIM) family of proteins is a class of highly conservative proteins that have been implicated in multiple processes. TRIM59, one member of the TRIM family, has now received recognition as a key regulator in the development and progression of human diseases. However, its role in human tumorigenesis has remained largely unknown. In this study, the effects of TRIM59 expression on cell proliferation and migration were investigated in human cervical cancer cells. The expression of TRIM59 in clinical cervical cancer tissues and cervical cancer cells was initially determined by RT-PCR and Western blot.… More >

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Xingtao Han^*†, Jinjian Yang^*, Zhankui Jia^*, Pengtao Wei^†, Han Zhang^†, Wenwei Lv^†, Jiantao Sun^†, Qingxiang Huo^†
Oncology Research, Vol.25, No.3, pp. 389-395, 2017, DOI:10.3727/096504016X14743324568129
Abstract The pre-mRNA splicing regulator serine–arginine protein kinase 1 (SRPK1), a member of the SR kinase family, plays an essential role in cancer development and various pathophysiological processes. However, its expression pattern and functions in renal cell carcinoma (RCC) remain unknown. Therefore, the aim of this study was to assess the role of SRPK1 in RCC. Our data showed that SRPK1 was significantly upregulated in human RCC tissues and cell lines. SRPK1 interference significantly inhibited the proliferation of RCC cells and inhibited tumor growth in vivo. In addition, SRPK1 interference also suppressed migration and invasion in More >

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Yong Zhou, Chuandong Yang, Kunpeng Wang, Xuefeng Liu, Quan Liu
Oncology Research, Vol.25, No.3, pp. 397-405, 2017, DOI:10.3727/096504016X14743337535446
Abstract Recently, microRNA (miR)-33b has been demonstrated to act as a tumor suppressor in osteosarcoma. However, the regulatory mechanism of miR-33b in osteosarcoma cell proliferation and migration remains largely unknown. In this study, real-time PCR showed that miR-33b was significantly downregulated in osteosarcoma tissues compared to their matched adjacent nontumor tissues. Its expression was also decreased in several common osteosarcoma cell lines, including Saos-2, MG63, U2OS, and SW1353, when compared to normal osteoblast cell line hFOB. Overexpression of miR-33b suppressed U2OS cell proliferation and migration. HIF-1α was further identified as a target of miR-33b, and its… More >

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Cheng Xu^*1, Lu Cao^*1, Jianhua Liu^†1, Zhongrun Qian^‡, Yu Peng^§, Wenjing Zhu^§, Yongming Qiu^‡, Yingying Lin^‡¶
Oncology Research, Vol.25, No.3, pp. 407-415, 2017, DOI:10.3727/096504016X14743350548249
Abstract Elevated circulating asparaginyl endopeptidase (AEP), a novel lysosomal protease, has been found in breast cancer, and AEP is thus considered to be a prognostic factor in this disease. However, the pathological functions of circulating AEP in the development of breast cancer and the potential of AEP-targeted therapy remain unclear. We used MMTV-PyVmT transgenic mice, which spontaneously develop mammary tumors. Western blotting showed overexpression of AEP in both primary tumor tissue and lung metastases compared to their normal counterparts. Moreover, the concentration of circulating AEP gradually increased in the serum during the development of mammary tumors. More >

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Feng Jiang¹, Dengfeng Zhang¹, Guojun Li, Xiao Wang
Oncology Research, Vol.25, No.3, pp. 417-425, 2017, DOI:10.3727/096504016X14747253292210
Abstract DDX46, a member of the DEAD-box (DDX) helicase family, is involved in the development of several tumors. However, the exact role of DDX46 in osteosarcoma and the underlying mechanisms in tumorigenesis remain poorly understood. Thus, in the present study, we explored the role of DDX46 in osteosarcoma and the underlying mechanisms. Our results demonstrated that the expression levels of DDX46 in both mRNA and protein were greatly elevated in human osteosarcoma tissues and cell lines. Knockdown of DDX46 obviously inhibited osteosarcoma cell proliferation and tumor growth in vivo. In addition, knockdown of DDX46 also significantly More >

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Xiaorui Liu^*†1, Jingjing Li^*1, Zujiang Yu^*, Juan Li^*, Ranran Sun^*, Quancheng Kan^‡
Oncology Research, Vol.25, No.3, pp. 427-435, 2017, DOI:10.3727/096504016X14747300207374
Abstract Hepatocellular carcinoma (HCC) is the most common cancer in the world. MicroRNAs (miRNAs) are a type of small noncoding RNA that can regulate the expression of target genes under physiological and pathophysiological conditions. Aberrant expression of microRNA-935 (miR-935) has been reported in cancer studies. However, its expression and mechanism in HCC remain unclear. In our study, we found that miR-935 was upregulated in liver cancer tissues and cells. Overexpression of miR-935 in liver cells promoted cell proliferation, tumorigenesis, and cell cycle progression, whereas inhibition of miR-935 reduced cell proliferation, tumorigenicity, and cell cycle progression. These… More >

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Erkun Guo, Hongjiang Liu, Xiaopeng Liu
Oncology Research, Vol.25, No.3, pp. 437-444, 2017, DOI:10.3727/096504016X14747335734344
Abstract Signal peptide CUB EGF-like domain-containing protein 2 (SCUBE2), a member of the SCUBE family of proteins, was recently found to play an important role in cancer development. However, little is known regarding its biological function in glioma. In the present study, we investigated the effect of SCUBE2 on glioma and explored its relevant mechanisms. The study showed that SCUBE2 had a low expression in glioma tissue and cell lines. SCUBE2 overexpression inhibited glioma cell proliferation in vitro and in vivo as well as suppressed glioma cell migration and invasion in vitro. Furthermore, we found that More >

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Ying Liu^*†, Hui Lv^‡, Xiaoying Wu^*§, Jun Zhou^¶, Ying Shi^#, Jifang Wen^*†
Oncology Research, Vol.25, No.3, pp. 445-454, 2017, DOI:10.3727/096504016X14747368729786
Abstract Breast cancer is the leading cause of cancer deaths in females all over the world, mainly resulting from metastasis. Previous studies have revealed that repressor element-1 (RE-1) silencing transcription (REST) acted as a tumor suppressor in breast cancer. However, the mechanism by which REST is regulated remains unknown, and its role in the metastasis in breast cancer cells remains unclear. In the present study, we showed that the expression of REST was lower in breast cancer samples than that of adjacent samples by immunohistochemical analysis, which may be due to hypermethylation of the REST promoter.… More >

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Jihong Sun^*†1, Jingjing Li^‡1, Weiguo Zhang^*, Juan Zhang^*, Shenjie Sun^*, Guiqi Li^*, Hengliang Song^*, Daguo Wan^*
Oncology Research, Vol.25, No.3, pp. 455-461, 2017, DOI:10.3727/096504016X14747283032017
Abstract Gastric cancer (GC) is the fourth most common cancer globally. Recently, microRNAs (miRNAs) have been suggested to be closely associated with tumorigenesis. Aberrant expression of miR-509-3p has been reported in cancer studies. However, the expression and mechanism of its function in GC remain unclear. Here we showed that miR-509-3p was downregulated in GC specimens, which was associated with overall survival. Functional investigations demonstrated that the overexpression of miR-509-3p inhibited the migration and proliferation of the GC cells. Additionally, we identified X-linked inhibitor of apoptosis protein (XIAP) as a direct target of miR-509-3p. Knockdown of XIAP More >

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