Open Access

ARTICLE

Overexpression of miR-140 Inhibits Proliferation of Osteosarcoma Cells via Suppression of Histone Deacetylase 4

Qianren Xiao^*1, Lu Huang^†1, Zhongzu Zhang^‡1, Xiang Chen^*, Jiaquan Luo^*, Zhanmin Zhang^§, Shaoqing Chen^§, Yong Shu^*, Zhimin Han^*, Kai Cao^*

* Department of Orthopedics, The First Affiliated Hospital of Nanchang University, Nanchang, P.R. China
† Department of Children Health and Care, Jiangxi Maternal and Child Health Hospital, Nanchang, Jiangxi, P.R. China
‡ Department of Orthopedics, The Yongchuan Hospital of Chongqing Medical University, Chongqing, P.R. China
§ Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, P.R. China
1 These authors provided equal contribution to this work.

Oncology Research 2017, 25(2), 267-275. https://doi.org/10.3727/096504016X14732510786564

Abstract

miRNAs play a pivotal role in the development and progression of osteosarcoma (OS). Previous studies indicated that miR-140 acts as a tumor suppressor in many cancers. However, its accurate expression and exact function in OS cells remain unknown. Herein, we demonstrated the lower expression of miR-140 in 40 paired OS tissues. Restoring miR-140 expression in OS cells had a marked effect on inhibiting cell proliferation and invasion, inducing cell apoptosis in vitro, and suppressing tumor growth in vivo. Moreover, a bioinformatics prediction indicated that the histone deacetylase 4 (HDAC4) is a target gene of miR-140 and is involved in miR-140-mediated suppressive effects. In conclusion, our findings show that miR-140 acts as a tumor suppressor in OS by targeting HDAC4.

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