Open Access
ARTICLE
Inhibition of Proliferation, Migration, and Invasion by Knockdown of Pyruvate Kinase-M2 (PKM2) in Ovarian Cancer SKOV3 and OVCAR3 Cells
Yi Miao1, Meng Lu1, Qin Yan, Shuangdi Li, Youji Feng
Department of Obstetrics and Gynecology, Shanghai General Hospital of Nanjing Medical University, Shanghai, P.R. China
Oncology Research 2016, 24(6), 463-475. https://doi.org/10.3727/096504016X14685034103671
Abstract
Pyruvate kinase (PK) is a key enzyme in the process of glycolysis, catalyzing phosphoenolpyruvate (PEP)
into pyruvate. Currently, PK isozyme type M2 (PKM2), one subtype of PK, has been proposed as a new
tumor marker with high expression in various tumor tissues. Here we aimed to explore the effects of siRNAPKM2 on ovarian carcinoma (OC) cell lines SKOV3 and OVCAR3, in which PKM2 was notably expressed.
PKM2 gene interference lentivirus vectors were built by miRNA transfection assay. siRNA-PKM2-transfected
SKOV3 and OVCAR3 cells were evaluated for cell proliferation, cell cycle distribution, cell apoptosis, cell
migration, and invasion in this study. In addition, the expression levels of several tumor-related genes were
measured using real-time PCR and Western blot. Results showed that siRNA-PKM2 markedly inhibited cell
proliferation, induced apoptosis, and caused cell cycle arrest at the G
0/G
1 phase. Cell migration and invasion
were significantly suppressed by siRNA-PKM2. Furthermore, the tumor-related genes caspase 7, Bad, and
E-cadherin were upregulated, while MMP2, HIF1a, VEGF, and MMP9 were depressed by siRNA-PKM2. The
function of siRNA-PKM2 on the biological behavior of OC cells indicated that PKM2 may also be a target for
treatment of OC.
Keywords
Cite This Article
APA Style
Miao, Y., Lu, M., Yan, Q., Li, S., Feng, Y. (2016). Inhibition of proliferation, migration, and invasion by knockdown of pyruvate kinase-m2 (PKM2) in ovarian cancer SKOV3 and OVCAR3 cells. Oncology Research, 24(6), 463-475. https://doi.org/10.3727/096504016X14685034103671
Vancouver Style
Miao Y, Lu M, Yan Q, Li S, Feng Y. Inhibition of proliferation, migration, and invasion by knockdown of pyruvate kinase-m2 (PKM2) in ovarian cancer SKOV3 and OVCAR3 cells. Oncol Res. 2016;24(6):463-475 https://doi.org/10.3727/096504016X14685034103671
IEEE Style
Y. Miao, M. Lu, Q. Yan, S. Li, and Y. Feng "Inhibition of Proliferation, Migration, and Invasion by Knockdown of Pyruvate Kinase-M2 (PKM2) in Ovarian Cancer SKOV3 and OVCAR3 Cells," Oncol. Res., vol. 24, no. 6, pp. 463-475. 2016. https://doi.org/10.3727/096504016X14685034103671