Open Access
ARTICLE
Knockdown of SPOCK1 Inhibits the Proliferation and Invasion in Colorectal Cancer Cells by Suppressing the PI3K/Akt Pathway
Ping Zhao*, Hai-Tao Guan†, Zhi-Jun Dai†, Yu-Guang Ma†, Xiao-Xu Liu†, Xi-Jing Wang†
* Department of Gastroenterology, the Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi Province, P.R. China
† Department of Oncology, the Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi Province, P.R. China
Oncology Research 2016, 24(6), 437-445. https://doi.org/10.3727/096504016X14685034103554
Abstract
Sparc/osteonectin, cwcv, and kazal-like domains proteoglycan (testican) 1 (SPOCK1), known as testican-1,
were found to be involved in the development and progression of tumors. However, in colorectal cancer (CRC),
the expression pattern of SPOCK1 and its functional role remain poorly investigated. In the present study,
we explored the role of SPOCK1 in CRC. Our results demonstrated that SPOCK1 is overexpressed in CRC
cell lines. SPOCK1 silencing significantly inhibited the proliferation in vitro and the tumor growth in vivo.
Furthermore, SPOCK1 silencing significantly attenuated the migration/invasion by reversing the EMT process
in CRC cells. Finally, knockdown of SPOCK1 obviously decreased the protein expression levels of p-PI3K and
p-Akt in HCT116 cells. In total, our study demonstrated for the first time that knockdown of SPOCK1 inhibits the
proliferation and invasion in CRC cells, possibly through the PI3K/Akt signaling pathway. Therefore, SPOCK1
may be a potential therapeutic target for the treatment of CRC.
Keywords
Cite This Article
APA Style
Zhao, P., Guan, H., Dai, Z., Ma, Y., Liu, X. et al. (2016). Knockdown of SPOCK1 inhibits the proliferation and invasion in colorectal cancer cells by suppressing the pi3k/akt pathway. Oncology Research, 24(6), 437-445. https://doi.org/10.3727/096504016X14685034103554
Vancouver Style
Zhao P, Guan H, Dai Z, Ma Y, Liu X, Wang X. Knockdown of SPOCK1 inhibits the proliferation and invasion in colorectal cancer cells by suppressing the pi3k/akt pathway. Oncol Res. 2016;24(6):437-445 https://doi.org/10.3727/096504016X14685034103554
IEEE Style
P. Zhao, H. Guan, Z. Dai, Y. Ma, X. Liu, and X. Wang "Knockdown of SPOCK1 Inhibits the Proliferation and Invasion in Colorectal Cancer Cells by Suppressing the PI3K/Akt Pathway," Oncol. Res., vol. 24, no. 6, pp. 437-445. 2016. https://doi.org/10.3727/096504016X14685034103554