Open Access

ARTICLE

miR-183 Modulates Cell Apoptosis and Proliferation in Tongue Squamous Cell Carcinoma SCC25 Cell Line

Dayong Yan^*1, Xiaoqing Cai^*1, Yu Feng^†

* Oral and Maxillofacial Surgery, The Central Hospital of Zhengzhou, University of Zhengzhou, Zhengzhou, Henan, P.R. China
† Anesthesia Medicine, The Central Hospital of Zhengzhou, University of Zhengzhou, Zhengzhou, Henan, P.R. China
1 These authors provided equal contribution to this work.

Oncology Research 2016, 24(6), 399-404. https://doi.org/10.3727/096504016X14685034103239

Abstract

This study was designed to investigate the role of miR-183 in modulating cell growth and apoptosis of tongue squamous cell carcinoma SCC25 cell line. Human squamous epithelial cell and squamous cell carcinoma cell line SCC25 was used, and miR-183 was inhibited. Cell growth, colony formation, and apoptotic rate, as well as the expression of caspase 3 and BCL-xL, were detected. Results showed that miR-183 was significantly overexpressed in the SCC25 cell line when compared with normal control. The miR-183 inhibitor reduced cell growth and colony formation, while the apoptosis percentage was significantly increased. The expression of activated caspase 3 and BCL-xL was obviously up- and downregulated in siRNA-transfected cells, respectively. In conclusion, miR-183 contributed to cell growth and proliferation, and suppressed cell apoptosis in SCC25 cells. Therefore, miR-183 might serve as a therapeutic target in tongue squamous cell carcinoma (TSCC).

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Keywords

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