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Si Jun Park^*1, Bo Ram Lee^*1, Hyeng-Soo Kim^*, Young Rae Ji^*, Yong Hun Sung^*, Kwang ShikChoi^*, Hum Dai Park^†, Sung-Hyun Kim^*, Myoung Ok Kim^‡, Zae Young Ryoo^*
Oncology Research, Vol.23, No.3, pp. 89-98, 2015, DOI:10.3727/096504015X14496932933539
Abstract In the present study, we found that lung cancer cell line (H460 cells) expressing Tet1 showed higher levels of adhesion, and Tet1 inhibited H460 cell proliferation. In addition, these cells showed a significantly reduced ability of collagen degradation and Smad2/3 phosphorylation compared to controls. Furthermore, vimentin was found to be highly expressed in larger metastatic cancer area. Tet1 overexpression was reduced in the epithelial marker E-cadherin. Moreover, Tet1 repressed cancer cell metastasis in nude mice. Collectively, these findings suggest that Tet1 expression plays a critical role in metastasis of lung cancer cells by suppression of More >

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Gao Liu^*, Tian Xiang^†, Quan-Feng Wu^‡, Wei-Xing Wang^*
Oncology Research, Vol.23, No.3, pp. 99-107, 2015, DOI:10.3727/096504015X14496932933575
Abstract The lncRNA H19 and its mature product miR-675 have recently been shown to be upregulated and promote the progression of gastric cancer. However, the detailed function and underlying molecular mechanism of H19/miR- 675 in the carcinogenesis of gastric cancer remains unclear. In this study, we found that H19 depended on miR- 675 to enhance the proliferation and invasion of gastric cancer AGS cells, and the expression of miR-675 was positively correlated with H19 in patients with gastric cancer. Subsequently, the tumor-suppressor runt domain transcription factor 1 (RUNX1) was confirmed to be a downstream molecule of… More >

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Fei Liu^*1, Bihua Lin^*1, Xin Liu^*, Wenzhang Zhang^*, Erying Zhang^*, Liang Hu^*, Yuefan Ma^*, Xiangyong Li^*, Xudong Tang^*†
Oncology Research, Vol.23, No.3, pp. 109-118, 2015, DOI:10.3727/096504015X14496932933610
Abstract Extracellular signal-regulated kinase (ERK)1/2 signaling pathway plays a critical role in regulating tumor angiogenesis. Our previous studies have demonstrated that HPV-16 oncoproteins enhanced hypoxia-inducible factor-1α (HIF-1α) protein accumulation and vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) expression in non-small cell lung cancer (NSCLC) cells, thus contributing to angiogenesis. In this study, we further investigated the role of ERK1/2 signaling pathway in HPV-16 oncoprotein-induced HIF-1α, VEGF, and IL-8 expression and in vitro angiogenesis in NSCLC cells. Our results showed that HPV-16 E6 and HPV-16 E7 oncoproteins promoted the activation of ERK1/2 signaling pathway in A549 More >

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Hao Lu¹, Chaoyang Sun¹, Ting Zhou, Bo Zhou, Ensong Guo, Wanying Shan, Meng Xia, Kezhen Li, Danhui Weng, Li Meng, Xiaoyan Xu, Junbo Hu, Ding Ma, Gang Chen
Oncology Research, Vol.23, No.3, pp. 119-128, 2015, DOI:10.3727/096504015X14496932933656
Abstract Drug resistance is the leading cause of chemotherapy failure in the treatment of ovarian cancer. So far, little is known about the mechanism of chemoresistance in ovarian cancer. In this study, we explored the mechanism that HSP27 was involved in cisplatin resistance of ovarian cancer both in vitro and clinically. HSP27 protein was found to be upregulated and expressed in cisplatin-resistant ovarian cancer cell line C13*, and HSP27 siRNA transfection reversed the chemoresistance of C13*. We found that HSP27 exerted its chemoresistant role by inhibiting p21 transferring from the nucleus to the plasma through the More >

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Qun Zhao, Bi-Bo Tan, Yong Li, Li-Qiao Fan, Pei-Gang Yang, Yuan Tian
Oncology Research, Vol.23, No.3, pp. 129-136, 2015, DOI:10.3727/096504015X14500513118029
Abstract In this study, the effects of hypoxia-inducible factor-1α (HIF-1α) on gastric carcinoma (GC) drug resistance through apoptosis-related genes are investigated. First, HIF-1α-specific siRNA was synthetized and transfected into drug-resistant GC cell line OCUM-2MD3/L-OHP. Then MTT assay was applied to test the inhibition rate of GC cells by 5-fluorouracil (5-FU) and oxaliplatin (L-OHP). After that, flow cytometry (FCM) was applied to measure apoptosis rate. qPCR and Western blot assay were employed to detect HIF-1α and apoptosis-related genes. Results showed that HIF-1α in OCUM-2MD3/L-OHP cells was higher than that in OCUM-2MD3 and gastric epithelial cells. After HIF-1α-siRNA More >

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Christine G. Roth^*, Amanda Gillespie-Twardy^†, Stanley Marks^†, Mounzer Agha^†, Anastasios Raptis^†, Jing-Zhou Hou^†, Rafic Farah^†, Yan Lin^§, Ying Qian^§, Liron Pantanowitz^‡, Michael Boyiadzis^†
Oncology Research, Vol.23, No.3, pp. 137-146, 2015, DOI:10.3727/096504015X14500972666761
Abstract “Double” or “triple” hit lymphomas (D/THL) with recurrent translocations involving MYC/8q24 and BCL2/18q21 and/or BCL6/3q27 are characterized by a poor prognosis, but their identification is hampered by the clinicopathologic overlap with other disease categories. Cases with circulating blastic-appearing cells may initially cause concern for lymphoblastic leukemia a diagnostic dilemma, which has not been well studied. There is only limited literature regarding the flow cytometric (FC) D/THL phenotype and its clinical correlates. The FC features of 20 D/THL (11 BCL2⁺/MYC⁺, 5 BCL6⁺/MYC⁺, 4 BCL2⁺/BCL6⁺/MYC⁺) were evaluated, compared to 20 B-lymphoblastic leukemias (B-LBL), and correlated with overall survival. Most (89%,… More >

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