Open Access
ARTICLE
Mir-612 Inhibits Proliferation and Invasion of Urothelial Carcinoma of Bladder Cells through Activating Hippo Pathway via Targeting PMEPA1
Peng Wang, Haiyang Zhang, Zilian Cui, Xunbo Jin*, Dong Zhang*
Department of Urology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250021, China
* Corresponding Authors: Xunbo Jin. Email: ; Dong Zhang. Email:
Oncologie 2021, 23(2), 259-268. https://doi.org/10.32604/Oncologie.2021.015503
Received 23 December 2020; Accepted 02 April 2021; Issue published 22 June 2021
Abstract
Urothelial carcinoma of bladder (UCB) is a common urological malignancy in the world, but its progression
mechanism remains unclear. MiR-612 was found as an anti-tumor factor in multiple types of cancer, while
few studies have revealed its functions in UCB cells. Based on this, UCB cells such as HTB-9 and HTB-4, and
normal urothelial of bladder cells such as SV-Huc1, were used as subjects in this study. Western blot, qRTPCR, CCK-8 assay and transwell assay were used to assess functions of miR-612 in UCB cells. The database,
miRWalk, was used to search for potential targets of miR-612, and dual-luciferase reporter assay was used to
verify the accuracy of the forecasting results. Besides, PMEPA1 overexpressed vector and miR-612 mimics were
co-transfected into HTB-4 to observe the regulation mechanism of miR-612. It was found that miR-612 was
significantly downregulated in HTB-9 and HTB-4 cells rather than in SV-Huc1 cells, and overexpressed
miR-612 reduced the proliferation and invasion of HTB-4 cells. The expression level of YAP1 was negatively related
with miR-612 while LATS1 was positively related with miR-612. Besides, the results of miRWalk and dual-luciferase
reporter assay indicated that PMEPA1 was a target of miR-612, and overexpression of PMEPA1 could reverse the
effects of miR-612 on UCB including weakened proliferation and invasion abilities, low expression level of YAP1 and
high expression level of LATS1. Therefore, this study suggests that miR-612 inhibits the proliferation and invasion of
urothelial carcinoma of bladder cells through activating Hippo pathway via targeting PMEPA1.
Keywords
Cite This Article
Wang, P., Zhang, H., Cui, Z., Jin, X., Zhang, D. (2021). Mir-612 Inhibits Proliferation and Invasion of Urothelial Carcinoma of Bladder Cells through Activating Hippo Pathway via Targeting PMEPA1.
Oncologie, 23(2), 259–268. https://doi.org/10.32604/Oncologie.2021.015503