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ARTICLE
Analysis of Antioxidant Potential of Trigonella foenum-graecum (L.) Extract Against Tumorigenesis
Shivangi Goyal, Sreemoyee Chatterjee, Nidhi Gupta*
Department of Biotechnology, IIS (Deemed to be University), SFS, Gurukul Marg, Mansarovar, Jaipur, Rajasthan, 302020, India
* Corresponding Author: Nidhi Gupta. Email:
# Contributed equally to the research work and manuscript
Oncologie 2021, 23(1), 89-104. https://doi.org/10.32604/Oncologie.2021.015234
Received 02 December 2020; Accepted 18 January 2021; Issue published 30 March 2021
Abstract
Background: Trigonella foenum-graecum Linn. has been extensively used for medicinal purposes. The current study deals with
in vitro and
in vivo correlation of free radical quenching activity and anticancer potential of seed extracts of
Trigonella.
Materials and methods: Antioxidant activity was evaluated against DPPH, NO and ABTS via
in vitro radical scavenging assay. Cytotoxicity effect of
Trigonella seed extract was studied in human embryonic kidney HEK 293 cell line by alamar blue assay.
In vivo antioxidant activity in Swiss albino mice model was assessed by studying endogenous antioxidant enzymes such as GSH, GPx, SOD and LPO. Antitumor effect was observed by studying parameters like total number of tumors, tumor size in mice. Further, expression of tumor suppressor gene, p53 in treated mice was investigated.
Results: In vitro antioxidant assays had shown methanolic extract to possess higher radical scavenging activity than aqueous and to be minimal cytotoxic.
In vivo study has shown a significant reduction in total number of tumors and tumor size for the mice group treated with extract in comparison to DMBA-TPA treated group. An increase in the levels of GSH, SOD and GPx was observed with a significant reduction in LPO levels. Expression of p53 was found to be upregulated in Swiss albino mice treated with extract emphasizing possible antitumor effect of
Trigonella.
Conclusion: The present study helped in understanding the reducing potential and antitumorigenic activity of
Trigonella seed extract and its probable therapeutic effect in skin papillomagenesis.
Keywords
Cite This Article
APA Style
Goyal, S., Chatterjee, S., Gupta, N. (2021). Analysis of antioxidant potential of trigonella foenum-graecum (L.) extract against tumorigenesis. Oncologie, 23(1), 89-104. https://doi.org/10.32604/Oncologie.2021.015234
Vancouver Style
Goyal S, Chatterjee S, Gupta N. Analysis of antioxidant potential of trigonella foenum-graecum (L.) extract against tumorigenesis. Oncologie . 2021;23(1):89-104 https://doi.org/10.32604/Oncologie.2021.015234
IEEE Style
S. Goyal, S. Chatterjee, and N. Gupta "Analysis of Antioxidant Potential of Trigonella foenum-graecum (L.) Extract Against Tumorigenesis," Oncologie , vol. 23, no. 1, pp. 89-104. 2021. https://doi.org/10.32604/Oncologie.2021.015234