Open Access

ARTICLE

Analysis of Antioxidant Potential of Trigonella foenum-graecum (L.) Extract Against Tumorigenesis

Shivangi Goyal, Sreemoyee Chatterjee, Nidhi Gupta^*

Department of Biotechnology, IIS (Deemed to be University), SFS, Gurukul Marg, Mansarovar, Jaipur, Rajasthan, 302020, India

* Corresponding Author: Nidhi Gupta. Email:
# Contributed equally to the research work and manuscript

Oncologie 2021, 23(1), 89-104. https://doi.org/10.32604/Oncologie.2021.015234

Received 02 December 2020; Accepted 18 January 2021; Issue published 30 March 2021

Abstract

Background: Trigonella foenum-graecum Linn. has been extensively used for medicinal purposes. The current study deals with in vitro and in vivo correlation of free radical quenching activity and anticancer potential of seed extracts of Trigonella. Materials and methods: Antioxidant activity was evaluated against DPPH, NO and ABTS via in vitro radical scavenging assay. Cytotoxicity effect of Trigonella seed extract was studied in human embryonic kidney HEK 293 cell line by alamar blue assay. In vivo antioxidant activity in Swiss albino mice model was assessed by studying endogenous antioxidant enzymes such as GSH, GPx, SOD and LPO. Antitumor effect was observed by studying parameters like total number of tumors, tumor size in mice. Further, expression of tumor suppressor gene, p53 in treated mice was investigated. Results: In vitro antioxidant assays had shown methanolic extract to possess higher radical scavenging activity than aqueous and to be minimal cytotoxic. In vivo study has shown a significant reduction in total number of tumors and tumor size for the mice group treated with extract in comparison to DMBA-TPA treated group. An increase in the levels of GSH, SOD and GPx was observed with a significant reduction in LPO levels. Expression of p53 was found to be upregulated in Swiss albino mice treated with extract emphasizing possible antitumor effect of Trigonella. Conclusion: The present study helped in understanding the reducing potential and antitumorigenic activity of Trigonella seed extract and its probable therapeutic effect in skin papillomagenesis.

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