Open Access
ARTICLE
RETRACTED: LncRNA TUG1 Targets miR-222-3p to Take Part in Proliferation and Invasion of Breast Cancer Cells
Yuqin Xie1, Shuang Deng1, Qian Deng2, Jiudong Xu*
1 Department of Cardiothoracic and Breast Surgery, The Second Affiliated Hospital of Chengdu Medical College, Chengdu, 610051, China
2 National Nuclear Corporation 416 Hospital, Chengdu, 610051, China.
* Corresponding Author: Jiudong Xu. Email:
Oncologie 2020, 22(3), 179-188. https://doi.org/10.32604/Oncologie.2020.012544
Abstract
This study aimed to explore LncRNA TUG1 targeted miR-222-3p in
the proliferation and invasion of breast cancer (BC) cells. Seventy-six BC patients
admitted to our hospital and 62 health check-ups at the same time were selected as
the research objects. Among them, the former was seen as the observation group
(OG), and the latter was considered as the control group (CG). The clinical
significance of LncRNA TUG1 and miR-222-3p in BC was detected. Human BC
cell MCF7 and normal human breast epithelial cell MCF-10A were purchased.
After cells were transfected with LncRNA TUG1 and miR-222-3p, their
proliferation, invasion, and apoptosis were observed, and the relationship between
the two was detected. LncRNA TUG1 was highly expressed, while miR-222-3p
was low expressed in BC. When the cut-off value (COV) was 2.109, the sensitivity
and specificity of detecting LncRNA TUG1 expression in peripheral blood for BC
diagnosis were 98.39% and 69.74%, respectively. When the COV was 0.760, the
sensitivity and specificity of detecting miR-222-3p expression were 67.11% and
91.94%. The cell proliferation and invasion of the sh-TUG1 group were
dramatically higher than those of the other two, while the apoptosis rate was lower
(
p < 0.05). The cell proliferation and invasion of the si-TUG1r group were lower
than those of the TUG1-NC group, while the apoptosis rate was higher (
p < 0.05).
The cell proliferation and invasion of the miR-222-3p-inhibitor group were
dramatically higher than those of the other two, while the apoptosis rate was lower
(
p < 0.05). The miR-222-3p-mimics group has lower cell proliferation and
invasion but higher apoptosis rate than NC group. (
p < 0.05) The LncRNA TUG1
and miR-222-3p had the same gene sequence after target gene verification by
http://starbase.sysu.edu.cn/. Biological behavior tests showed no difference in cell
proliferation, invasion and apoptosis between the sh-TUG1 + miR-222-3p-mimics
group and the TUG1-NC group (
p > 0.05). TUG1 promotes the proliferation,
invasion and inhibit apoptosis of BC cells by targeted regulation of miR-222-3p.
Keywords
Cite This Article
Xie, Y., Deng, S., Deng, Q., Xu, J. (2020). RETRACTED: LncRNA TUG1 Targets miR-222-3p to Take Part in Proliferation and Invasion of Breast Cancer Cells.
Oncologie, 22(3), 179–188. https://doi.org/10.32604/Oncologie.2020.012544