Open Access

ARTICLE

Expression Changes, Prognostic Analysis and Risk Factors of miR-625-3p and miR-449a in Osteosarcoma Patients after Surgery

Hui Zhang, Zhan Wang, Lin Liu, Fuqiang Zhang, Yuxin Song, Yaowen Qian^*

Department of Orthopaedics, Gansu Provincial Hospital, Lanzhou, 730000, China

* Corresponding Author: Yaowen Qian. Email:

Oncologie 2020, 22(1), 23-33. https://doi.org/10.32604/oncologie.2020.012493

Abstract

The expression changes of miR-625-3p and miR-449a in osteosarcoma patients after surgery is the critical study in this paper. Analysis of their prognosis and risk factors can be helpful in understanding the prognostic value for clinical purposes. Fifty-eight patients with osteosarcoma diagnosed in our hospital were considered as the research group (RG), and 52 health subjects at the same time were collected as the control group (CG). Fluorescence quantitative PCR (RTPCR) was employed to test the expression levels of miR-625-3p and miR-449a in the serum of subjects in both groups before surgery and patients in the RG after surgery. The survival conditions of both groups with high and low miR-625-3p and miR-449a expression levels were compared after 5-year follow-up of patients in the RG. The prognostic factors of osteosarcoma patients were assessed through Cox regression analysis. The miR-625-3p expression level in the RG was dramatically higher than that in the CG (p < 0.05), and the miR-449a expression level was dramatically lower than that in the CG (p < 0.05). The miR-625-3p expression level in the RG after surgery was markedly lower than that before surgery (p < 0.05), and the miR-449a expression level was markedly higher than that before surgery (p < 0.05). After they were followed up for 5 years, the overall survival rate of those in the RG was 60.34%, the rate of miR-625-3p low expression group (LEG) was dramatically higher than that of high expression group (HEG) (p < 0.05), and the rate of miR-449a HEG was markedly higher than that of miR-449a LEG (p < 0.05). The relative expression of miR-625-3p and miR- 449a was remarkably different from tumor size, lymph node metastasis, and Enneking stage (p < 0.05). Univariate Cox regression analysis identified that tumor size, lymph node metastasis, Enneking stage, miR-625-3p and miR-449a were risk factors affecting the prognosis of patients; multivariate Cox regression analysis found that the five were independent prognostic factors of them. miR-625-3p and miR-449a are differentially expressed in the postoperative serum of osteosarcoma patients, and their differential expression is an independent risk factor affecting their prognosis, which has prognostic value and can provide a reference value for clinical practice.

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Keywords

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