TY - EJOU AU - Wang, Yequan AU - Yang, Li AU - Zhang, Jin AU - Xue, Ruyue AU - Tang, Zhenyu AU - Huang, Wei AU - Jiang, Dianming AU - Tang, Xinyan AU - Chen, Peter AU - Sung, KL Paul TI - Differential MMP-2 Activity Induced by Mechanical Compression and Inflammatory Factors in Human Synoviocytes T2 - Molecular \& Cellular Biomechanics PY - 2010 VL - 7 IS - 2 SN - 1556-5300 AB - The anterior cruciate ligament, posterior cruciate ligament, cartilage and meniscus in human knee joint have poor healing ability. Accumulation of MMPs in the joint fluids due to knee injury has been considered as the main reason. Our previous experiments showed that synovium may be the major regulator of MMPs in joint cavity after injury. In this paper, we used human synoviocytes harvested from synovium to determine whether mechanical injury and inflammatory factors will induce MMP-2 production in synoviocytes. With zymography, we found that mechanical compression increased the MMP-2 production by 23% under 6% compressions, 61% under 12% compression and 109% under 14% compressions. In addition, TNF-acan also elevate the activity of MMP-2 in a dose dependent manner, while IL-1α does not. However, mixture of these two factors dramatically increased MMP-2 production by 201%. In addition, mechanical injury had a strong synergistic effect on MMP-2 production with TNF-α, IL-1α and their mixture, increasing by 207%, 354% and 468% individually. The generic MMP activity assay revealed that mechanical compression increased the generic activity. APMA treatment increased the generic activity of MMPs induced by compression but not inflammatory factors, which indicates that compression would induce MMPs in pro-form while inflammatory factors induce MMPs mostly in active-form. We concluded that mechanical injury and inflammation would induce large amount of MMP-2 and global MMP activity in synoviocytes, which is always injured accompanied by the injury of other joint tissues. The accumulation of MMPs and the elevated proteolytic activities in the comparative isolated joint cavity would destroy the balance of tissue repairs, which may be the major reason why joint tissues have poor healing ability. Thus, synvoium may be involved in regulating the micro-environment of joint cavity and the injury/healing process of knee joint tissue. KW - Synoviocytes KW - compression KW - TNF-α KW - IL-1α KW - MMP-2 DO - 10.3970/mcb.2010.007.105