Open Access

ARTICLE

Injectable Collagen/CMC Soft Tissue Filler with Developed Flow Properties

Reza Samanipour¹, Aida Pourmostafa², Adel Marzban², Sara Tabatabaee³, Hasan Bahraminasab², Amir Ali Hamidieh⁴, Amirhossein Tavakoli^4,*

1 Department of Tissue Engineering and Applied Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Department of Research and Development Iranian Tissue Product Co., Tehran, Iran
3 Department of Bio-Computing, Faculty of Interdisciplinary Sciences and Technologies, Tarbiat Modares University, Tehran, Iran
4 Iranian Tissue Bank & Research Center, Gene, Cell, and Tissue Research Institute, Tehran University of Medical Sciences, Tehran, Iran

* Corresponding Author: Amirhossein Tavakoli. Email:

Molecular & Cellular Biomechanics 2022, 19(2), 97-104. https://doi.org/10.32604/mcb.2022.019080

Received 01 September 2021; Accepted 30 December 2021; Issue published 25 March 2022

Abstract

Based on the remarkable demand for facial reconstitute or reshape fillers due to the dermal defects arising from specific diseases, trauma, or aging, several natural or synthetic materials have been investigated. Among the evaluated materials, decellularized dermis is one of the most biocompatible choices for the aim of skin tissue regenerative approaches. On the other hand, Carboxymethyl Cellulose (CMC), a synthetic polysaccharide, with the desirable degradability, biomechanical stability, and nontoxicity seems to be an acceptable reinforcement agent for decellularized dermis. Thus, in this research, an injectable soft tissue filler contained of human-derived decellularized collagen and CMC was fabricated. The cell-removal approving was performed utilizing H&E staining assay. The biocompatibility of the prepared samples was confirmed by MTT assay. The rheology examination demonstrated the increased storage modulus and enhanced elastic property as a consequence of CMC presence. Furthermore, the required flow force of the collagen/CMC filler was decreased as a consequence of decreasing the viscosity and its injectability was improved. According to the provided biomechanical and biological results, it could be claimed that the collagen/CMC hydrogel is a suitable substitute filler for skin tissue engineering.

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Keywords

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