Vol.17, No.4, 2020, pp.199-204, doi:10.32604/mcb.2020.011811
OPEN ACCESS
ARTICLE
Toxicity Evaluation of Geniposide on MCF-7 Cancer Cells
  • Kena Lv1, Shuangshuang Zheng2, Xiangqin Li1, Yi Nie2,3,*, Tianqing Liu1, Kedong Song1,*
1 State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian, 116024, China
2 Zhengzhou Institute of Emerging Industrial Technology, Zhengzhou, 450000, China
3 Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China
* Corresponding Authors: Kedong Song. Email: Kedongsong@dlut.edu.cn; Yi Nie. ynie@ipe.ac.cn
Received 30 May 2020; Accepted 17 September 2020; Issue published 20 October 2020
Abstract
As one of the adjuvant treatments for cancer treatment, traditional Chinese medicine treatment has a wide range of cancer treatments, such as preventing metastasis and relapse, improving the efficacy of radiotherapy and chemotherapy, reducing the side effects of chemotherapy, improving body function, extending life and improving the life quality. Geniposide (GEN) is a bioactive substance extracted from the fruit of gardenia. In recent years, it has attracted attention due to its anti-tumor effect. In this study, we aimed to investigate whether GEN could inhibit the proliferation of human breast cancer cells (MCF-7) and promote its apoptosis. The half-inhibitory concentration (IC50) values of GEN were firstly determined as 16.06, 14.85 and 13.14 mg/mL by the CCK-8 experiment after cells treated for 24 h, 48 h, and 72 h, respectively. The inhibitory effect of GEN on MCF-7 cells was in concentration- and time-dependent manners from the results of CCK-8 experiment and Live/Dead staining. AO/EB staining result has shown that GEN has induced MCF-7 cell apoptosis.
Keywords
MCF-7; geniposide; anti-cancer
Cite This Article
Lv, K., Zheng, S., Li, X., Nie, Y., Liu, T. et al. (2020). Toxicity Evaluation of Geniposide on MCF-7 Cancer Cells. Molecular & Cellular Biomechanics, 17(4), 199–204.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.