TY - EJOU
AU - Nguyen-Thi, Phuong-Thao
AU - Le, Quoc Phong
AU - Stoldt, Volker R.
AU - Tran, Ngoc Quyen
AU - Le, Anh Tho
AU - Huynh, Khon
TI - Morphologies of Fibronectin Fibrils Formed under Shear Conditions and Their Cellular Adhesiveness Properties
T2 - Molecular \& Cellular Biomechanics
PY - 2020
VL - 17
IS - 3
SN - 1556-5300
AB - Fibrillar fibronectin (FFN) is a biological active form of FN which form
linear and branched meshwork around cells and support cellular activities. Previous studies have demonstrated that shear stress can induce cell-free FN fibrillogenesis. In this study, we further examined the effect of shear stress conditions on
morphology of formed FFN and preliminarily looked for relationship between
FFN’s morphology and cell adhesion. Plasma FN at 50 µg/ml was perfused
through channel slides at shear rates of 500 s-1 or 4000 s-1. Our results showed
that there were four FFN structures formed: (1) FN nodules, (2) fibril in different
sizes (3) with or without nodule attachment, and (4) fibrillar matrix. At 4000 s-1,
FFN fibrils was formed within the first 10 min and reached the highest surface
coverage only after 20 min. In contrast, FFN formation was significant more
slowly at 500 s-1 at which only FN nodules and small fibrils were formed. Platelets bound on thin layer of FN and rarely found on large FN fibrils. In contrast,
fibroblast stretched their shape on platform of FFN matrix and bound actively
to all types of FFNs. Taken together, our data suggests a morphological dependent
biological activity of FFN.
KW - Shear condition; fibrillar fibronectin; cell-free fibrillogenesis
DO - 10.32604/mcb.2020.09643