Open Access
ARTICLE
Covalent and Ionic Bonding between Tannin and Collagen in Leather Making and Shrinking: A MALDI-ToF Study
Antonio Pizzi*
LERMAB-ENSTIB, University of Lorraine, Epinal, 88000, France
* Corresponding Author: Antonio Pizzi. Email:
Journal of Renewable Materials 2021, 9(8), 1345-1364. https://doi.org/10.32604/jrm.2021.015663
Received 03 January 2021; Accepted 18 January 2021; Issue published 08 April 2021
Abstract
Collagen powder hydrolysates were reacted with a solution of commercial mimosa bark tannin extract. The mixture was prepared at ambient temperature and prepared at 80°C to determine what reactions, if any, did occur
between the collagen protein through its amino acids and the polyphenolic condensed tannin. The reaction products obtained were analyzed by matrix assisted laser desorption ionization time-of-flight (MALDI ToF) mass
spectrometry. Reactions between the two materials did appear to occur, with the formation of a relatively small
proportion of covalent and ionic linkages at ambient temperature but a considerable proportion of covalent linkages tannin-protein amino acids and the disappearance of ionic bonds. The linkages between the two materials
appeared to be by amination of the phenolic –OHs of the tannin by the amino groups of the non-skeletal side
chains of arginine, and by esterification by the –COOH groups of glutamic and aspartic acid of the aliphatic
alcohol-OH on the C3 site of the flavonoid units heterocycle of the tannin. The proportion of covalent linkages
increases markedly and predominate with increasing temperatures. This tightening of the tannin-protein covalent
network formed may be an additional contributing factor both to leather wear resistance and performance as well
to leather shrinking when this is subjected to excessive temperatures.
Keywords
Cite This Article
Pizzi, A. (2021). Covalent and Ionic Bonding between Tannin and Collagen in Leather Making and Shrinking: A MALDI-ToF Study.
Journal of Renewable Materials, 9(8), 1345–1364. https://doi.org/10.32604/jrm.2021.015663
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