Open Access

ARTICLE

Identification of Phenolic Compounds from K. ivorensis by Selected Chromatographic and Spectrometric Techniques

by A. Bikoro Bi Athomo^1,2, S. P. Engozogho Anris^1,2, R. Safou Tchiama^2,3, F. Eyma⁴, C. Arnaudguilhem⁵, B. Charrier^1,*

1 CNRS, Université de Pau et des Pays de l’Adour, Institut des Sciences Analytiques et de Physico-Chimie pour l’Environnement et les Matériaux-Xylomat, UMR5254, Mont de Marsan, France
2 Laboratoire de Recherche et de Valorisation du Matériau Bois (LaReVa Bois), Ecole Normale Supérieure d’Enseignement Technique (ENSET), Libreville, Gabon
3 Laboratoire des Substances Naturelles et de Synthèses Organométalliques (LASNSOM), Unité de Recherche en Chimie, Université des Sciences et Techniques de Masuku, Franceville, Gabon
4 Institut Clément Ader (ICA), Université de Toulouse, CNRSUMR 5312-INSA-ISAE-Mines Albi-UPS, Tarbes, France
5 CNRS, Univ Pau & Pays de l’Adour/ E2S UPPA, Institut des Sciences Analytiques et de Physicochimie pour l’Environnement et les Matériaux, UMR5254, Pau, France

* Corresponding Author: B. Charrier. Email:

Journal of Renewable Materials 2021, 9(1), 35-48. https://doi.org/10.32604/jrm.2021.013626

Received 14 August 2020; Accepted 19 October 2020; Issue published 30 November 2020

Abstract

A complementary approach using Liquid Chromatographic-Mass Spectrometric analysis was proposed to characterize phenolic compounds from the methanol-water extracts of K. ivorensis A. Chev. Two High Performance Liquid Chromatography (HPLC) complementary methods were used for the determination of phenolic compounds from the bark, sapwood and heartwood of K. ivorensis. Methods employed involved direct analysis after filtration at 0.20 µm, using a RP C₁₈ column and UV-VIS/ESI-FTMS detection. The methods used were different by their elution gradient and allowed analyzing the chemical composition of three parts of African mahogany extracts. In this study, 22 phenolic compounds and derivatives from K. ivorensis were separated, determined or tentatively characterized for the first time based on their methanol/water/formic acid extract mass spectra. The difference of gradients eluted various compounds, 8 were obtained with the first method and 14 with the second one. The main products were hydroxybenzoic acid and derivatives, resorcinol, esterified compounds, mannitol, quercetin and derivatives, dihydroxyflavan, and trihydroxyflavan.

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Keywords

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