Open Access

ARTICLE

Two Routes to Produce Chitosan from Agaricus bisporus

Abdelghani Hassainia¹, Hamid Satha^1,*, Boufi Sami²

1 LSPN, University of 8 Mai 1945, BP 401, Guelma, 24000, Algeria
2 Faculty of Science-LMSE, University of Sfax, Sfax, 3000, Tunisia

* Corresponding Author: Hamid Satha. Email:

Journal of Renewable Materials 2020, 8(1), 101-111. https://doi.org/10.32604/jrm.2020.07725

Received 21 June 2019; Accepted 19 August 2019; Issue published 01 January 2020

Abstract

Two methods were used to produce chitosan by deacetylation of chitin which was extracted from Agaricus bisporus stipes. The first one gives chitosan 1 with low yield of 2.5%, degree of acetylation (DA) of 4%, molecular weight (MW) of 2.973 × 10⁵ (g/mol). The second route produces chitosan 2 with higher yield of 41%, degree of acetylation (DA) of 17.23%, molecular weight (MW) of 2.939 × 10⁵ (g/mol). Both chitosans were characterized by XRD, FTIR, ¹ H-NMR spectroscopy nuclear magnetic resonance of proton. The molecular weight (M_W) was determined by size exclusion chromatography (SEC). Thermal analysis shows that both chitosans have moisture content lower than 10%. However chitosan 2 has the less ash % which is the quality grade for chitosan medical applications. Accordingly, fungal chitosan 2 could have potential medical and agricultural applications.

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