Open Access

ARTICLE

Blend of Polyhydroxyalkanoates Synthesized By Lipase Positive Bacteria From Plant Oils

by Nasir Javaid, Rida Batool, Nazia Jamil*, Rida Batool, Nazia * Jamil*^*

Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore-54590, Pakistan.

*Corresponding Author: Nazia Jamil. Email: .

Journal of Renewable Materials 2019, 7(5), 463-476. https://doi.org/10.32604/jrm.2019.00023

Abstract

A total of 5 biochemically characterized lipase positive bacterial strains were screened for Polyhydroxyalkanoates (PHA) production by Nile blue staining and confirmation was done by Sudan Black B. PHA production ability for all strains was optimized followed by time profiling calculation and comparison via using glucose and two plant oils i.e., canola and mustard oil. Quantitative analysis showed that glucose can serve as a carbon source for maximum biomass (2.5 g/L CDW for strain 5) and PHA production (70.3% for strain 2). PHA produced by strain 2 was further analyzed for its chemical composition and type via Fourier Transform Infrared (FT-IR) spectroscopy. It revealed homopolymer (PHB) and copolymer (PHB-co-PHV) production of PHA (peaks at 1743 cm-1 and 2861 cm-1, respectively) with both canola and mustard oil unlike glucose which produced only homopolymer one i.e., PHB (peaks at 1110 cm-1, 1411 cm-1 and 1650 cm-1). Crystallinity of FT-IR analyzed PHA was calculated using mathematical formulas which showed decrease from glucose to canola to mustard oil. This study revealed that plant oils can serve as better carbon source to produce better quality (ductile and copolymer) PHA. Moreover, 16S rRNA gene sequencing analysis showed that strain 1, strain 2, strain 3, strain 4 and strain 5 are Stenotrophomonas sp. N3, Exiguobacterium sp. N4, Exiguobacterium sp. Ch3, Cellulosimicrobium sp. A8 and Klebsiella sp. LFSM2, respectively.

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