Open Access

ARTICLE

Growth of Non-enzymatic Cholesterol Biosensor using TiO₂ Decorated Graphene Oxide with Bare GCE and PPy-GCE

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1 Department of Physics, Sri Ramakrishna Engineering College, Vattamalaipalayam, Coimbatore-641022, Tamil Nadu, India.
2 Department of Physics, Sri Ramakrishna Mission Vidyalaya College of Arts and Science, Coimbatore-641020, Tamil Nadu, India.
3 Department of Physics,Bannari Amman Institute of Technology, Sathymangalam, Erode-638401,Tamil Nadu, India.
4 Department of Physics, Dr. N. G. P. Arts and Science College Coimbatore- 641048 Tamil Nadu, India.
5 Department of Physics, Sri Eshwar College of Engineering, Coimbatore-641202, Tamil Nadu, India.

* Corresponding Author: e-mail:

Journal of Polymer Materials 2021, 38(3-4), 295-307. https://doi.org/10.32381/JPM.2021.38.3-4.10

Abstract

The cholesterol level determination is a significant clinical diagnostic solution for heart and, thrombosis problems. In this work, we examined a novel non-enzymatic cholesterol biosensor using cholesterol oxidase (ChOx) enzyme immobilized on TiO₂ nanoparticles influenced by reduced graphene oxide (rGO) - polypyrrole (PPy) (rGO-TiO₂ /PPy-GCE) nanocomposite was developed on a glassy carbon electrode (GCE) and the higher sensing response with lower detection limits were observed. The electrochemical properties of GCE modified PPy (PPy-GCE) were studied using CV (Cyclic Voltammetry) and DPV (Differential Pulse Voltammetry). The reported sensor exhibited piecewise linearity in the range of 0.1 µM to 1 µM and 1 µM to 600 µM with the sensing response of 61.665 and 0.1466 (2μA μM×cm) respectively. The detection limits of the sensor were found to be 32 nm. The results were repeatable and reproducible and this sensor can be applied to determine the cholesterol at real sample with satisfactory results.

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