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ARTICLE
Taguchi Design of the Experimental approach to Increase the Biomass and Cell Wall Chitosan Contents of Zygomycetous Dimorphic Fungus Benjaminiella Poitrasii
S.R. MANE1,2, E. K. PATHAN3, G. PATIL4, S. G. TUPE5, V. GHORMADE4, B. P.
CHAUDHARI1,2, M.V. DESHPANDE5,*
1
Biochemical Sciences Division, CSIR-National Chemical Laboratory, Pune-411008, India.
2
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad-201002, India.
3 Department of Biosciences and Bioengineering, Indian Institute of Technology-Bombay,
Powai, Mumbai-400076, India.
4
Nanobioscience, Agharkar Research Institute, Pune-411004, India.
5
Greenvention Biotech Pvt. Ltd., Pune-412202, India.
* Corresponding Author: e-mail:
Journal of Polymer Materials 2021, 38(3-4), 219-230. https://doi.org/10.32381/JPM.2021.38.3-4.4
Abstract
A dimorphic fungus Benjaminiella poitrasii contains high chitin/chitosan (35% of the cell wall) in
the mycelial (M) form than its yeast (Y) form (20% of the cell wall). However, the relative proportion
of chitosan is more in yeast form cells (chitosan: chitin ratio, 6:1) than mycelial cells (chitosan:
chitin ratio, 3:1). Using the Taguchi design of experimental (DOE) approach, interactions among
eight different parameters showed that carbon source (starch, 10 g/L), incubation time (48 h),
inoculum (M and Y mixed 10%), yeast extract (6 g/L) and peptone (10 g/L), were optimum for
maximum biomass production. Under these conditions, the chitosan yield from the mycelia was
60.89±2.30 mg/g of dry biomass, while from the yeast cells was 28.29±2.7 mg/g. The molecular
weights of chitosan isolated from M and Y cells were 41.28 kDa and 21.72 kDa, respectively as
measured by gel permeation chromatography. The degree of deacetylation of chitosans as
88.17-90.72% DDA measured by
1
H-NMR. Furthermore, chitosans from M and Y cells inhibited
the growth of plant pathogenic Fusarium oxysporum (MIC
900.1 and 0.4 mg/mL) and Ustilago
maydis (MIC
900.4 and 0.8 mg/mL) at lower concentrations as compared to chitosan isolated
from marine sources (MIC
90 0.8 and >1.6 mg/mL).
Keywords
Cite This Article
APA Style
MANE, S., PATHAN, E.K., PATIL, G., TUPE, S.G., GHORMADE, V. et al. (2021). Taguchi design of the experimental approach to increase the biomass and cell wall chitosan contents of zygomycetous dimorphic fungus benjaminiella poitrasii. Journal of Polymer Materials, 38(3-4), 219-230. https://doi.org/10.32381/JPM.2021.38.3-4.4
Vancouver Style
MANE S, PATHAN EK, PATIL G, TUPE SG, GHORMADE V, P.
CHAUDHARI B, et al. Taguchi design of the experimental approach to increase the biomass and cell wall chitosan contents of zygomycetous dimorphic fungus benjaminiella poitrasii. J Polym Materials . 2021;38(3-4):219-230 https://doi.org/10.32381/JPM.2021.38.3-4.4
IEEE Style
S. MANE et al., "Taguchi Design of the Experimental approach to Increase the Biomass and Cell Wall Chitosan Contents of Zygomycetous Dimorphic Fungus Benjaminiella Poitrasii," J. Polym. Materials , vol. 38, no. 3-4, pp. 219-230. 2021. https://doi.org/10.32381/JPM.2021.38.3-4.4