Open Access

ARTICLE

Covalent Immobilization of Lipase on Novel Nanofibrous Membrane for Catalysis of the Organic Synthesis

YINCHUN FANG^a, XINHUA LIU^a,b,*, XU YANG^a, CUIE WANG^a,*

a College of Textile and Clothing, Anhui Polytechnic University, Wuhu 241000, China;
b Technology Public Service Platform for Textile Industry of Anhui Province, Wuhu 241000, China

* Corresponding Authors: e-mail: ,

Journal of Polymer Materials 2019, 36(2), 111-119. https://doi.org/10.32381/JPM.2019.36.02.1

Abstract

Enzymes are green biocatalysts which have been widely used in many fields. Immobilization enzymes on nanofibrous membrane possessed easy recycling and high stability which would broaden their applications. Covalent immobilization of lipase could endow them higher stability than other protocols. In this study, a novel nanofibrous membrane containing epoxy groups and hydrophilic polyethylene oxide branch was used as a support for lipase immobilization. The immobilized lipase was used as the biocatalyst to catalyse Rap. stroermer reaction. The results showed that it obtained the high product yield of 88% when the volume ratio of methanol and water was 4:1, the dosage of immobilized lipase was 40~50 mg, the reaction temperature and time were 30~35 °C and 10 h.

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Keywords

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