Phyton-International Journal of Experimental Botany

About the Journal

Phyton-International Journal of Experimental Botany is an international journal that publishes on the broadest aspects of plant biology and ecology. The journal welcomes the original and exciting submissions that provide new and fundamental insights into the origins, development, and function of plants from the molecular to the whole organism and its interactions within the biotic and abiotic environment. Phyton-International Journal of Experimental Botany publishes outstanding research in the plant and ecology sciences, especially in the areas of plant physiology and biochemistry, plant metabolism, plant ecology and evolution, as well as those making use of synthetic, modeling, bioinformatics, and -omics tools. Manuscripts submitted to this journal must not be under simultaneous consideration or have been published elsewhere, either in part or in whole.

Indexing and Abstracting

Thomson Scientific; Science Citation Index Expanded (SCIE); Journal Citation Report/Science Edition (JCR); Impact Factor (2019): 0.329; 5-Year Impact Factor: 0.373; Biological Abstracts; BIOSIS Previews; Scopus; EMBiology; Latindex; Field Crop Abstracts; CAB Abstracts; CABI Full Text; Periódica; TEEAL, The Essential Electronic Agricultural Library; Núcleo Básico de Revistas Científicas Argentinas (Agosto 2013–Julio 2015); SciELO (Scientific Electronic Library Online); Portal de Revistas en Biodiversidad; Portico, etc...

  • Non-Canonical Functions of the E2F/DP Pathway with Emphasis in Plants
  • Abstract The E2F/DP pathway is a widely conserved regulatory mechanism in pluricellular organisms. The family of E2F and DP transcription factors was originally described having a role in the transition from the G1 to the S phase of the cell cycle. However, the discovery of hundreds of possible gene targets and their involvement in many other biochemical processes, soon showed that they participated in cell development and differentiation, chromatin remodeling, DNA repair and others. The E2F/DP transcription factors can act as either activators or repressors of transcription depending on their association to other regulatory proteins, particularly the retinoblastoma protein, or even… More
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  • Applications of Decision Support System: A Case Study of Solanaceous Vegetables
  • Abstract Crop simulation models constitute the major proportion in decision support systems. A large number of crop models have been developed for potato and few for tomato and peppers. In the literature, thirty three crop models have been reported to simulate potato, nine for tomato and six for peppers. Some of these models dealt with the climate change scenario and others with the crop management practices such as sowing time, irrigation, nitrogen, and insect-pests management. The most evaluated and applied models for potato include; SUBSTOR, and LINTUL-Potato, whereas CROPGRO-tomato model is the most tested and applied for tomato. The AQUACROP is… More
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  • Effects of Exogenous α-Ketoglutaric Acid on 2-Acetyl-1-Pyrroline, Yield Formation and Grain Quality Characters of Aromatic Rice
  • Abstract The improvement of grain quality in aromatic rice is very important for farmer to increase their income. Present study was conducted with a two-year field experiment and three aromatic rice cultivars in order to study the effects of exogenous α-ketoglutaric acid on yield formation, grain quality characters and the biosynthesis of 2-acetyl-1-pyrroline (2-AP, key component of aromatic rice’s fragrance) in aromatic rice. At heading stage, 0.50 mmol L–1 (T1) and 1.00 mmol L–1 (T2) α-ketoglutaric acid solutions were overhead sprinkle to aromatic rice plants, respectively while the treatment which was overhead sprinkled with distilled water was set as control (CK).… More
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  • Character Identification of an Early Flowering Mutant
  • Abstract The concept of gene-function-genetic trait was introduced to explore the effects of early flowering on the growth and development of maize at the jointing stage and to obtain early flowering mutants using ethyl methanesulfonate mutagenesis. First, we studied gene expression, phytohormones, and lignin content to explore the physiological peculiarities of the early flowering mutant. Then we analyzed the genetic features of the mutants during the jointing stage by measuring physiological and biochemical indices of drought tolerance. The results showed that the photosynthetic rate of the mutant was significantly higher than that of the control and the rate of accumulation of… More
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  • Polysaccharide Elicitor from the Endophyte Bionectria sp. Fat6 Improves Growth of Tartary Buckwheat under Drought Stress
  • Abstract Drought can limit the growth and reduce the yield of crops, but the safe and effective bio-approach to improve the drought resistance of crops is very little. We conducted an experiment in which we monitored the effects of polysaccharide from the endophyte Bionectria sp. Fat6 on the growth of Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) seedlings under control and drought-stressed conditions by determining gas exchange, photosynthesis parameters, photosynthetic pigment contents, and metabolite accumulation. Results indicated that the polysaccharide from endophyte stimulated plant growth and increased the aboveground biomass, root mass, and root/shoot ratio of Tartary buckwheat. Application of the polysaccharide… More
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  • Doubled Haploid Production Using an Improved Anther Culture Protocol for Sorghum [Sorghum bicolor (L.) Moench]
  • Abstract Sorghum [Sorghum bicolor (L.) Moench] can benefit from accelerated breeding and release of improved varieties through doubled haploid technology. The technology has been used in speeding up the breeding of other major cereals such as wheat, maize and rice, for which generally widely applied optimised protocols exist. A reproducible protocol for the crop, that can overcome genotype dependency and other species-specific challenges such as phenolic exudation is however lacking. This study aimed at sorghum doubled haploids production thereby contributing to the development of an improved protocol. From the 28 hybrid genotypes, both F1 registered- and experimental hybrids involved, this study… More
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  • Ameliorative Role of Pre-Sowing Proline Treatment in Coriandrum sativum L. Seedlings under Mercury Toxicity
  • Abstract Heavy metal toxicity is one of the major ecosystem concerns globally in present time and is also responsible for significant threat to agronomic crops. The current work was conducted to investigate the possible ameliorative role of proline in Coriandrum sativum L. seedlings treated with mercury (Hg). The seedlings were exposed to different concentrations of Hg (0, 0.1, 0.3 and 0.5 mM) for 20 days. The effects of pre-sowing treatment with proline were studied on C. sativum seedlings in terms of pigment (chlorophylls, carotenoids and anthocyanins), malondialdehyde (MDA), antioxidant compound (glutathione, total phenolic compounds, ascorbic acid) and osmolytes (proline, glycine betaine).… More
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  • Foliar Application of Phosphorus Enhances Photosynthesis and Biochemical Characteristics of Maize under Drought Stress
  • Abstract Water is essential for the growth period of crops; however, water unavailability badly affects the growth and physiological attributes of crops, which considerably reduced the yield and yield components in crops. Therefore, a pot experiment was conducted to investigate the effect of foliar phosphorus (P) on morphological, gas exchange, biochemical traits, and phosphorus use efficiency (PUE) of maize (Zea mays L.) hybrids grown under normal as well as water deficit situations at the Department of Agronomy, University of Agriculture Faisalabad, Pakistan in 2014. Two different treatments (control and P @ 8 kg ha−1 ) and four hybrids (Hycorn, 31P41, 65625,… More
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  • Benzyl Amino Purine and Gibberellic Acid Coupled to Nitrogen-Limited Stress Induce Fatty Acids, Biomass Accumulation, and Gene Expression in Scenedesmus Obliquus
  • Abstract The need for renewable energy sources makes microalgae an essential feedstock for biofuels production. The molecular aspects and the response to nitrogen (N)-limited conditions with a phytohormone stimulus in microalgae have been slightly explored. In this work, Scenedesmus obliquus was used as a study model to analyze the effect of benzyl amino purine (BAP) and gibberellic acid (GA) coupled to nitrogen limitation on cell growth, biomass and fatty acids. The selected 10-5 M BAP increased the biomass by 1.44-fold, and 10-6 M GA by 1.35-fold. The total lipids also increased by 2.8 and 1.11-fold, respectively. The 10-5 M BAP and… More
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  • Evaluation of Ocimum basilicum L. with Different Concentrations of K+ as an Inhibitor of Pathogenic Bacterial Strains
  • Abstract The extraction of bioactive compounds has become one of the most interesting areas of modern chemistry. For therapeutic reasons, it´s important to obtain antimicrobial agents from natural origin. The objective of the present study was to determine the inhibitory effect of ethanolic extract of basil (Ocimum basilicum L. var. Red Rubin) subjected to different concentrations of potassium (K+) on the activity of three bacterial strains that are pathogens in humans. Susceptibility was evaluated by inhibition surface and these results were compared to two antibiotics: Gentamicin (GE) and Ciprofloxacin (CPF) for their efficacy against each bacterial strain. Analyzed variables presented significant… More
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  • Establishment of Rhodiola quadrifida Hairy Roots and Callus Culture to Produce Bioactive Compounds
  • Abstract Rhodiola quadrifida is a rare mountain medicinal plant whose root extracts are used in traditional Chinese medicine as a hemostatic, antitussive, and tonic in the treatment of gynecological diseases. The aim of the study was to obtain R. quadrifida cultures at different degrees of differentiation in vitro and compare their growth characteristics and the content of salidroside and rosavin. Hairy roots were obtained by incubating cotyledons and hypocotyls in a suspension of Agrobacterium rhizogenes strain A4. The presence of the rolB and rolC genes was proven by polymerase chain reaction. The obtained roots were cultivated in Murashige-Skoog medium (MS). Calluses… More
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  • Anthocyanin Profiles in Grape Berry Skins of Different Species of Wine Grapes in Shanxi, China
  • Abstract To understand the anthocyanin characteristics of wine grape varieties, the anthocyanin composition and content of 31 wine grape varieties were analyzed to explore the use of anthocyanins as chemical fingerprints to distinguish varieties. Results showed that a total of 21 anthocyanins were detected in the skins, including cyanidin, delphinidin, petunidin, peonidin and malvidin 3-monoglucosides (or 3,5-diglucosides) along with the corresponding acetyl and p-coumaroyl derivatives. The highest and lowest total amount of anthocyanins were detected in ‘Ruby Cabernet’ and ‘Muscat Rouge’, respectively. In the 21 Vitis vinifera grapes, there were 3~11 monoglucoside anthocyanins detected, however, there were 4 to 9 monoglucoside… More
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  • Efficient Evergreen Plant Regeneration of Cinnamomum japonicum Sieb. through in vitro Organogenesis
  • Abstract Cinnamomum japonicum Sieb. is an excellent roadside tree and medicinal tree species with considerable ornamental and economic value. In this study, we successfully developed a large-scale micropropagation protocol for C. japonicum for the first time. Sterilized shoots were excised and used as explants for shoot induction on several basal media, supplemented with different concentrations of plant growth regulators (PGRs), such as Thidiazuron (TDZ), N6 -Benzyladenine (6-benzylaminopurine) (BA), α-naphthaleneacetic acid (NAA) and Gibberellic acid (GA3). After comparison, the most efficient medium for shoot regeneration was 1/2 Murashige and Skoog (MS) medium containing 0.5 mg L–1 BA, 0.05 mg L–1 NAA and… More
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  • Optimization of Factors Influencing Adventitious Rooting in Hybrid Larch
  • Abstract Optimization of in vitro adventitious root induction contributes to the development of a large-scale production system of hybrid clone seedlings of larch (Larix spp.). We used orthogonal testing to investigate the factors that affect the rooting of hybrid larch shoots–activated carbon, vitamin B1, sucrose, glycine, glutamic acid (Glu), cysteine, pH, and induction time in the dark and indole-3-butyric acid (IBA). Variance analysis showed that the effects of sucrose concentration on rooting rate, survival rate, and average root number, pH on the average number of roots; and Glu on survival rate were all significant (p < 0.05) when compared to the… More
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  • An Efficient Plant Regeneration System of Hydrangea bretschneideri Dipp via Stem Segments as Explants
  • Abstract Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration. Genetic engineering technology has been successfully used in many plant species, but it is limited in Hydrangea. Here we established an efficient regeneration system by using stem segments as explants for the first time. In our study, the plant growth regulators (PGRs) were evaluated at the different regeneration processes, including axillary shoots regeneration and root induction. We found that the optimal concentration for axillary buds’ induction was 2.0 mgL−1 6-BA and 0.5 mgL−1 1 IAA, its highest induction rate was 70%. Moreover, the highest axillary shoots proliferation coefficient… More
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  • Interaction of Acaena elongata L. with Arbuscular Mycorrhizal Fungi under Phosphorus Limitation Conditions in a Temperate Forest
  • Abstract The aim of this study was to analyze the performance of Acaena elongata colonized by arbuscular mycorrhizal fungi (AMF) to different phosphorus (P) concentrations, as a measure of AMF dependency. A. elongata, is a species from soils where P availability is limited, such as temperate forests. Our research questions were: 1) How do different P concentrations affect the AMF association in Acaena elongata, and 2) How does the AMF association influence A. elongata’s growth under different P concentrations? A. elongata’s growth, P content in plant tissue, AMF colonization and dependency were measured under four P concentrations: control (0 g P… More
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  • Detection and Discrimination of Tea Plant Stresses Based on Hyperspectral Imaging Technique at a Canopy Level
  • Abstract Tea plant stresses threaten the quality of tea seriously. The technology corresponding to the fast detection and differentiation of stresses is of great significance for plant protection in tea plantation. In recent years, hyperspectral imaging technology has shown great potential in detecting and differentiating plant diseases, pests and some other stresses at the leaf level. However, the lack of studies at canopy level hampers the detection of tea plant stresses at a larger scale. In this study, based on the canopy-level hyperspectral imaging data, the methods for identifying and differentiating the three commonly occurred tea stresses (i.e., the tea leafhopper,… More
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  • Degree of Humification in Fresh Litter from a Subtropical Mixed Plantation Forest in Southwest China
  • Abstract The humification degree of fresh litter directly controls the accumulation of soil humus derived from plant litter, but very little information on this process is available. Planted forests are well known to restrict soil fertility, which is often indicated by the soil humus level. In this study, fresh litter was collected during different plant phenological stages during 2016 and 2017 in a mixed plantation in Southwest China. The values of hue coefficient ΔlogK (absorbance ratio of 400 nm and 600 nm on a logarithmic scale), optical density E4/E6 (absorbance ratio of 400 nm and 600 nm) and A600/C (absorbance at… More
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  • Poultry Manure as an Organic Fertilizer with or without Biochar Amendment: Influence on Growth and Heavy Metal Accumulation in Lettuce and Spinach and Soil Nutrients
  • Abstract This pot-based study investigated the influence of poultry manure and 1:1 mixture of poultry manure + biochar (produced from farmyard manure [FYM] or wood), on the biomass production and concentration of heavy metals in leaves of lettuce and spinach. The concentration of mineral nitrogen (N) and soluble inorganic phosphorus (P) of soils cultivated with these vegetables was also investigated. The application of poultry manure or FYM biochar in soil as 10% (equivalent to 60 t ha–1 , an estimated 1726.8 kg ha–1 N in poultry manure and 1353.9 kg ha–1 N in FYM) and 15% amendment (equivalent to 90 t… More
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  • Natarajania thailandica sp. nov. (Stilbosporaceae, Diaporthales) from Thailand
  • Abstract A fungus similar to the monotypic genus Natarajania, isolated from dead wood and collected in Thailand, is reported. Analysis of partial ribosomal LSU and a protein coding gene (RPB2) demonstrated that the new isolate belonged to Stilbosporaceae, Diaporthales and genetically different from N. indica. It is unique in producing synnematous conidiophores, smooth-walled conidiogenous cells and a flared collarette but lacks an elongated collar-canal which is distinct in the type species. Therefore, sequence data and morphological traits are used to introduce the new species, Natarajania thailandica. More
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