Open Access
ISSN:0031-9457(print)
ISSN:1851-5657(online)
Publication Frequency:Monthly
Phyton-International Journal of Experimental Botany is an international journal that publishes research on all aspects of plant science. This journal welcomes original and exciting submissions that provide new and key insights into the origins, growth and development of plants from the molecular to the whole organism and its interactions with the biotic and abiotic environments.
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Open Access
REVIEW
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 539-560, 2025, DOI:10.32604/phyton.2025.061776 - 31 March 2025
（This article belongs to the Special Issue: Emerging Insights into Phytohormonal Crosstalk in Plant Stress Tolerance)
Abstract Cytokinins are plant hormones that are essential for plant growth and development and are involved in a variety of processes. They are synthesized by the modification of adenine with an isoprenoid chain, resulting in cytokinins such as isopentenyladenine and zeatin. The levels of these hormones are regulated by conjugation, degradation and oxidation processes that modulate their activity. Cytokinins are perceived by cells through specific receptors that, when activated, trigger signaling cascades responsible for regulating the expression of genes critical for development. In addition, cytokinins interact with other hormones, such as auxins, to coordinate plant growth… More >
Open Access
REVIEW
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 561-581, 2025, DOI:10.32604/phyton.2025.061262 - 31 March 2025
（This article belongs to the Special Issue: Soil Microbes and Abiotic Stress Factors: Impacts on Root Physiology, Crop Growth, and Hormonal Dynamics)
Abstract Strawberry (Fragaria ananassa) is well known among consumers because of its attractive color, delicious taste, and nutritional benefits. It is widely grown worldwide, but its production has become a significant challenge due to changing climatic conditions that lead to abiotic stresses in plants, which results in poor root development, nutrient deficiency, and poor plant health. In this context, the major abiotic stresses are temperature fluctuations, water shortages, and high levels of soil salinity. The accumulation of salts in excessive amounts disrupts the osmotic balance and impairs physiological processes. However, drought reduces fruit size, yield, and quality.… More >
Open Access
REVIEW
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 583-601, 2025, DOI:10.32604/phyton.2025.063086 - 31 March 2025
（This article belongs to the Special Issue: Plant and Environments)
Abstract Glutathione S-transferases (GSTs) represent a large and diverse enzyme family ubiquitously distributed across the plant kingdom. These proteins catalyze the conjugation of glutathione (GSH) with electrophilic substrates in response to various stress conditions. Beyond their role in stress adaptation, certain GSTs are integral regulators of plant growth and development, contributing to a range of physiological processes. Most GST proteins exhibit dual enzymatic activities, functioning as both transferases and peroxidases, which enables their involvement in diverse cellular processes, including detoxification and stress responses. Recent advancements, particularly in X-ray crystallography, have enabled detailed structural analysis of GST More >
Open Access
REVIEW
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 603-621, 2025, DOI:10.32604/phyton.2025.062560 - 31 March 2025
（This article belongs to the Special Issue: Soil Microbe-Plant Interactions: Unveiling Mechanisms of Biostimulants in Stress Tolerance)
Abstract Soil metal pollution is a global issue due to its toxic nature affecting ecosystems and human health. This has become a concern since metals are non-biodegradable and toxic. Most of the reclamation methods currently used for soils rely on the use of physical and chemical means, which tend to be very expensive and result in secondary environmental damage. However, microbe-aided phytoremediation is gaining attention as it is an eco-friendly, affordable, and technically advanced method to restore the ecosystem. It is essential to understand the complex interaction between plants and microbes. The primary function of plant… More >
Graphic Abstract
Open Access
REVIEW
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 623-640, 2025, DOI:10.32604/phyton.2025.063763 - 31 March 2025
（This article belongs to the Special Issue: Plant Responses to Abiotic Stress Mechanisms)
Abstract Various environmental stressors, such as salinity, heat, drought, and metals, present significant obstacles to crop productivity. This study delves into the adverse effects of metals, specifically focusing on cadmium (Cd), nickel (Ni), mercury (Hg), chromium (Cr), arsenic (As), lead (Pb), and copper (Cu) on plants. It explores the sources of these metals, examining both natural occurrences and human-induced activities, and investigates the mechanisms through which plants absorb them. Metal pollution, in particular, negatively affects plant and microbiome well-being, producing reactive oxygen species (ROS) that harm essential macromolecules. Traditional stress-resistant plant varieties necessitate substantial development, leading… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 641-655, 2025, DOI:10.32604/phyton.2025.062871 - 31 March 2025
（This article belongs to the Special Issue: Plant Responses to Abiotic Stress Mechanisms)
Abstract The biochemical response of Coffea arabica var. Borbon to chitosan and chitosan oligosaccharides (COS) was evaluated in one-year-old plants under greenhouse conditions. COS solutions were synthesized through chemical and physical hydrolysis using acetic acid, hydrogen peroxide, and microwave irradiation. The obtained COS had an average molecular weight (Mw) of 3549.90 ± 0.33 Daltons (Da), a deacetylation degree (DD) of 76.64 ± 1.12%, and a polymerization degree (PD) of 18.91 ± 0.0018. Solutions of chitosan and COS were applied to C. arabica var. Borbon at concentrations of 0.25, 0.5, and 1 wt%. The experimental design was conducted using… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 657-677, 2025, DOI:10.32604/phyton.2025.062289 - 31 March 2025
（This article belongs to the Special Issue: Innovative Approaches in Experimental Botany: Essential Oils as Natural Therapeutics)
Abstract Juniperus oxycedrus (J. oxycedrus) is a traditional culinary spice and medicinal herb with a longstanding history of ethnopharmacological applications across diverse cultures. While prior research has explored the biological activities and phytochemical constituents of extracts derived from its leaves and seed cones, the present study systematically investigates their mineral and phenolic profiles alongside their multifunctional bioactive potential. Inductively coupled plasma-atomic emission spectroscopy (ICP-AES) analysis revealed a substantial abundance of essential macro- and microelements. Reversed-phase high-performance liquid chromatography (RP-HPLC) further identified high concentrations of phenolic acids (e.g., p-coumaric acid) and flavonoids (e.g., rutin and quercetin). The extracts exhibited More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 679-693, 2025, DOI:10.32604/phyton.2025.062196 - 31 March 2025
（This article belongs to the Special Issue: Plants Abiotic and Biotic Stresses: from Characterization to Development of Sustainable Control Strategies)
Abstract Coffee wilt represents one of the most devastating diseases of Arabica coffee (Coffea arabica L.) plantations in the primary coffee-producing regions. In this study, coffee trees manifesting wilt symptoms accompanied by the defoliation and drying of the whole tree were observed in the Jazan, El Baha, Najran, and Asir regions. The purpose of this investigation was to isolate and identify the Fusarium species recovered from symptomatic coffee trees. The developed fungi were initially characterized based on their morphological features followed by molecular phylogenetic multi-locus analysis of the combined sequences of ITS, TEF1-α, RPB2, and CaM. Twenty-five isolates… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 695-707, 2025, DOI:10.32604/phyton.2025.061790 - 31 March 2025
Abstract To promote the growth of cutting seeding of poplar (Populus L.), nitrogen (N) fertilizer and surface weed managements were required. We here conducted a pot experiment to examine the effects of natural vegetation, barnyardgrass (Echinochloa Beauv.), and sesbania (Sesbania cannabina pers.) on the growth of poplar cutting seedlings, soil properties, and ammonia (NH3) volatilization under three N inputs (0, 0.5, and 1.5 g/pot, i.e., N0, N0.5, and N1, respectively). Results showed that N application promoted the growth of poplar cutting seedlings, including plant height, ground diameter, and biomass, compared with N0 treatment. Moreover, under N0, sesbania significantly increased… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 709-721, 2025, DOI:10.32604/phyton.2025.062281 - 31 March 2025
（This article belongs to the Special Issue: Plant and Environments)
Abstract Plant invasion poses a serious environmental challenge to freshwater ecosystems. Heavy metal pollution and nutrient enrichment, particularly nitrogen (N) and phosphorus (P), are two additional critical environmental issues known to influence the growth of invasive plants. Notably, the N/P ratio in the environments is often inconstant; however, whether these variations can cause differential responses in invasive plants remains underexplored, despite its crucial importance for understanding plant invasiveness. In this study, we examined the effects of different N/P ratios (4:1, 16:1, 32:1, 64:1) on the growth of a widespread invasive plant Alternanthera philoxeroides in cadmium (Cd) polluted… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 723-737, 2025, DOI:10.32604/phyton.2025.063403 - 31 March 2025
（This article belongs to the Special Issue: Innovative Approaches in Experimental Botany: Essential Oils as Natural Therapeutics)
Abstract Traditional Palestinian medicine uses Thymus capitatus (T. capitatus), a plant recognized for its therapeutic properties due to its high concentration of essential oils such as thymol and carvacrol, to treat skin diseases, gastrointestinal disorders, and respiratory infections. The present study was conducted to evaluate the antioxidant and anticancer activities of T. capitatus essential oil (EO). Moreover, this study employed computational methods including ADMET analysis and molecular docking. Using Gas chromatography-mass spectrometry (GC-MS) analysis, the phytochemical composition of T. capitatus essential oil was identified. The DPPH scavenging method was used to assess antioxidant activity. The Michigan Cancer Foundation-7 (MCF-7) and… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 739-762, 2025, DOI:10.32604/phyton.2025.060712 - 31 March 2025
（This article belongs to the Special Issue: Influence of Biotic and Abiotic Stresses Signals on Plants and their Performance at Different Environments)
Abstract Brassica napus L. (B. napus), recognized as a significant cash and oil crop, faces challenges in popularization and application in northern China due to its limited cold resistance. Clarifying the mechanism of cold stress on gene regulation and signal transduction in B. napus is crucial. To address these issues, we conducted transcriptome sequencing and gene expression analysis, along with gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway profiling under natural (25°C) and cold (4°C) conditions in cold tolerant 16VHNTS309 and weak cold-resistant Tianyou 2238 B. napus seedlings. Enhanced genomic annotation was achieved through additional sequencing.… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 763-779, 2025, DOI:10.32604/phyton.2025.062717 - 31 March 2025
Abstract The increasing frequency and intensity of drought caused by climate change necessitate the implementation of effective ways to increase the ability of wheat to withstand drought, with humic acid being a promising approach. Therefore, a pot experiment was conducted to determine the efficacy of exogenous humic acid on wheat under water deficit stress via a completely randomized design (CRD) with three replications. The impacts of four growing conditions, i.e., well water (65% field capacity), water deficit stress (35% field capacity), soil application of humic acid (44 mg kg−1 soil) under water deficit stress and foliar feeding… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 781-791, 2025, DOI:10.32604/phyton.2025.062171 - 31 March 2025
（This article belongs to the Special Issue: Functional Plant Extracts and Bioactive Metabolites)
Abstract The use of conventional herbal medicines is a rapidly expanding phenomenon in developed nations. For instance, 30%–50% of all drug use in China is attributed to traditional herbal preparations. Current study evaluated the antioxidant (DPPH, FRAP), total phenolic and flavonoid content, antibacterial activity, and DNA damage protective potential of aqueous and methanolic extracts obtained from the aerial parts and roots of Cymbocarpum wiedemannii Boiss., an endemic plant in Turkey. In antioxidant analyses, the methanolic extract of the aerial parts showed the highest %DPPH (73.38) and IC50 (3.46 mg/mL) values. The FRAP analysis revealed the highest iron-reducing… More >
Graphic Abstract
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 793-807, 2025, DOI:10.32604/phyton.2025.063549 - 31 March 2025
Abstract Lycium ruthenicum Murray, a plant widely cultivated in northwestern China, is integral to traditional Chinese medicine, with applications in treating menstrual disorders, cardiovascular diseases, and menopausal symptoms. Despite its recognized medicinal value and use as a functional food, comprehensive knowledge of its metabolites and their pharmacological effects remains limited. This study presents an innovative approach using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC–QTOF/MS) to conduct a detailed analysis of both wild and cultivated L. ruthenicum samples. A total of 62 peaks were detected in the total ion current profile, with 59 metabolites identified based… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 809-824, 2025, DOI:10.32604/phyton.2025.062683 - 31 March 2025
（This article belongs to the Special Issue: Innovative Approaches in Experimental Botany: Essential Oils as Natural Therapeutics)
Abstract Pimpinella anisum, commonly known as anise, is generally used in both folk medicine and the culinary world. In traditional medicine, it is valued for its digestive, respiratory, and antispasmodic properties. This study aims to examine the volatile compounds and antibacterial effect of P. anisum essential oil (PAEO) as well as for the first time its genotoxicity employing both in vitro and computational approaches. Gas chromatography-mass spectrometry (GC-MS) analysis identified anethole as the principal compound, which comprises 92.47% of PAEO. PAEO was tested for its potential antibacterial properties against Bacillus subtilis ATCC 6633, Listeria innocua ATCC 33090, Staphylococcus aureus ATCC 29213, Klebsiella aerogenes… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 825-842, 2025, DOI:10.32604/phyton.2025.062015 - 31 March 2025
（This article belongs to the Special Issue: Influence of Biotic and Abiotic Stresses Signals on Plants and their Performance at Different Environments)
Abstract In plants, transcription factor (TF) family LATERAL ORGAN BOUNDARIES DOMAIN (LBD) has been identified to be involved in the ripening and senescence processes of fruits. However, the function of LBD in Hylocereus undatus (Haw.) Britton & Rose (H. undatus) has not been reported yet. Through transcriptomic analysis, virus-induced gene silencing (VIGS) technology, and RT-qPCR validation, we investigated the role of the LBD TFs in the senescence of H. undatus. Transcriptomic analysis revealed that HuLBD1 is a key transcription factor of the LBD family regulating H. undatus senescence. After silencing HuLBD1, 5075 differentially expressed genes (DEGs) were identified. GSEA results showed… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 843-860, 2025, DOI:10.32604/phyton.2025.063062 - 31 March 2025
Abstract Fertilization is essential for high yield and quality in Chinese herbs. Grona styracifolia (Osbeck) H. Ohashi, a distinctive medicinal plant in the Lingnan region, currently encounters cultivation issues stemming from the overuse of chemical fertilizers. Adopting organic and microbial fertilizers presents a sustainable solution for its cultivation management. This study compared a no-fertilization control group with eight treatment groups using various concentrations of compound, organic, compound microbial, and Bacillus amyloliquefaciens fertilizers to evaluate their effects on G. styracifolia and soil microbial communities. The results demonstrated that the different fertilization treatments significantly enhanced plant growth and quality of G. styracifolia,… More >
Graphic Abstract
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 861-873, 2025, DOI:10.32604/phyton.2025.062410 - 31 March 2025
（This article belongs to the Special Issue: Abiotic Stress in Agricultural Crops)
Abstract Exogenous proline is an effective agent for increasing plant tolerance to abiotic stress in plants. In this study, we evaluated its effect on seedlings of Siete Caldos chili pepper (Capsicum frutescens), a semi-domesticated variety. The Capsicum genus is known for its sensitivity to water stress. We pretreated the seedlings’ roots by immersing them in proline solutions (0, 2.5, 5, 7.5, and 10 mM) for 48 h. Then, we exposed them to water stress using a Hoagland nutrient solution supplemented with 10% polyethylene glycol (PEG-8000) for nine days. We analyzed key physiological and biochemical parameters, including relative… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 875-895, 2025, DOI:10.32604/phyton.2025.061550 - 31 March 2025
Abstract Mildew resistance locus O (MLO) proteins are extensively found in various plant species and are essential for multiple biological functions. The characterization and analysis of MLO genes have been conducted across numerous species. However, the functions and features of MLO genes inside sugar beet remain poorly understood. In the present research, we conducted a comprehensive analysis of the structural features of MLO genes, physicochemical characteristics of proteins, evolutionary connections, and expression profiles in sugar beet. A total of 13 BvMLO genes containing MLO structural domains were detected and renamed based on their locations on chromosomes within the sugar… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 897-910, 2025, DOI:10.32604/phyton.2025.063093 - 31 March 2025
Abstract The common fig (Ficus carica L.), Moraceae family, is a commonly grown fruit tree in the Mediterranean region. Fig mosaic disease (FMD) poses a substantial threat to Saudi Arabia’s fig-producing economy. A survey was conducted during the two seasons of 2021 and 2022 on fig trees, displaying various fig mosaic disease symptoms. A total of 200 fig leaves and fruit samples were collected from various governorates in several Regions of Saudi Arabia including Riyadh, Tabuk, Hail, Qassim, Al-Jouf, Makkah, Jazan, Al-Madinah, Asir, and Northern Borders. These samples were tested by reverse transcription polymerase chain reaction (RT-PCR) with… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 911-927, 2025, DOI:10.32604/phyton.2025.061770 - 31 March 2025
Abstract The use of a stable reference gene is fundamental for achieving reliable quantitative qRT-PCR (qPCR) results. Developing and evaluating the stability of reference genes is necessary for studying the molecular mechanisms of M. transitoria in response to drought stress. In this study, 18 candidate reference genes were selected from transcriptome sequencing data of M. transitoria according to their FPKM values under different drought stress degrees. Cluster-23533.34641 was identified as the most stable reference gene for M. transitoria under drought stress based on qPCR results and combined analysis of Genorm, NormFinder, BestKeeper, and Delta Ct algorithms. The reference genes More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 929-951, 2025, DOI:10.32604/phyton.2025.059288 - 31 March 2025
Abstract Chromium (Cr), a persistent soil pollutant, has detrimental effects on plants and living things, and its contamination in soil increased as a result of human-induced activities. Pakistan suffers from a lack of fresh water supplies; hence most people use metal-containing water and wastewater to irrigate their crops. Exposure to Cr toxicity, the plant reduces their morphological and physiological growth which ultimately decreases crop productivity. The current study was designed to investigate the foliar application of hesperidin (HSP) at varying effluent rates (25, 50, 75, and 100 mg L−1) on wheat growth under tannery wastewater irrigated soil.… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 953-971, 2025, DOI:10.32604/phyton.2025.060634 - 31 March 2025
（This article belongs to the Special Issue: Optimization of Propagation Techniques and Improvement of the Production and Quality of Red Fruit)
Abstract The objective of this study was to determine the optimal proportions of plant growth regulators for growth and non-tube rapid propagation of Cymbidium tortisepalum var. longibracteatum; seedlings were utilized as the material. The effects of various combinations and concentrations of 6-benzylaminopurine (6-BA), gibberellic acid (GA3), and naphthaleneacetic acid (NAA) on growth and non-tube rapid propagation were assessed through a single-factor testing and response surface methodology. The results indicated that 6-BA at 60 mg/L, GA3 at 150 mg/L, and NAA at 30 mg/L were the most effective concentrations for promoting leaf buds formation in the single-factor analysis. Response surface… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 973-986, 2025, DOI:10.32604/phyton.2025.062421 - 31 March 2025
Abstract Anisodus tanguticus (Maxim.) Pascher, a medicinal plant in the Solanaceae family, is widely distributed across the Qinghai-Tibet Plateau. Its medicinal properties, particularly the tropane alkaloids, are influenced by various ecological factors, but the underlying ecological mechanisms remain poorly understood. This study aimed to investigate how key environmental variables influence both the morphological traits and tropane alkaloid contents of A. tanguticus, with the goal of providing data to support the sustainable cultivation and management of this species. We collected samples from 71 sites across its natural habitat, analyzing the effects of factors such as soil nutrients, altitude, and… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 987-1006, 2025, DOI:10.32604/phyton.2025.062351 - 31 March 2025
（This article belongs to the Special Issue: Plant Biodiversity (Cultivated and Wild Flora) and Its Utility in Plant Breeding)
Abstract The medicinal herb Geranium wallichianum belongs to the family Geraniaceae. The East Asian Himalayas are its primary habitat. Overexploitation and overharvesting pose a threat to this plant, given its extensive ethnomedical utilization in the community. In Pakistan, its population has already declined by over 75%. Given its critical medicinal importance, urgent conservation efforts are needed to prevent extinction. The aim of the current research was to determine the effectiveness of sucrose and polyethylene glycol (PEG) in decelerating the growth of this medicinally important species. Nodal segments were utilized as explant with varying levels of polyethylene glycol… More >
Open Access
ARTICLE
Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 1007-1027, 2025, DOI:10.32604/phyton.2025.061270 - 31 March 2025
（This article belongs to the Special Issue: Advances in Molecular Insights of Plant Secondary Metabolites: Biosynthesis, Regulation, and Applications)
Abstract Cornus capitata, commonly known as the Himalayan strawberry, is a dogwood species from the family Cornaceae. Dogwood plants have been used extensively to practice conventional medicine due to their biological activities; however, the mechanisms behind these activities, particularly in C. capitata, remain largely unexplored. This study focuses on the preliminary phytochemical screening, antioxidant activity, anti-diabetic potential, and antimicrobial profiling of different extracts (i.e., leaves, bark, and fruit) from C. capitata to evaluate its potential pharmaceutical and medicinal applications. The in-vitro antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay, Ferric-reducing antioxidant power (FRAP) assay, and Superoxide Anion Scavenging assay.… More >
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