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ARTICLE

miR-208a Promotes Apoptosis in H9c2 Cardiomyocytes by Targeting GATA4

Liying Gong1,2,3, Hongkun Jiang4, Guangrong Qiu1, Kailai Sun1,*
1 Department of Medical Genetics, School of Life Sciences, China Medical University, Shenyang, China
2 Department of Laboratory Medicine, The People’s Hospital of Liaoning Province, Shenyang, China
3 Department of Laboratory Medicine, The People’s Hospital of China Medical University, Shenyang, China
4 Department of Pediatrics, The First Affiliated Hospital of China Medical University, Shenyang, China
* Corresponding Author: Kailai Sun. Email:

Congenital Heart Disease 2021, 16(5), 499-512. https://doi.org/10.32604/CHD.2021.015831

Received 19 January 2021; Accepted 26 February 2021; Issue published 03 June 2021

Abstract

Background: microRNAs are crucial for cardiovascular development and are associated with congenital heart disease (CHD). Recent studies have shown that microRNAs play a role in heart development and is closely related to CHD. The present study investigated the underlying mechanism of microRNA-208a (miR-208a) in “simple” CHD. Material and Methods: Reverse transcription-quantitative PCR (RT-qPCR) demonstrated miR-208a expression levels in children with CHD (n = 27) compared with normal controls (n = 29), in cardiomyocytes from embryo 10 (E10) to post-birth (P7) and organs in adult rats in healthy rats. Apoptosis of H9c2 cells after transfection with miR-208a detected by TUNEL assay. B-cell lymphoma (Bcl)-2, an anti-apoptotic gene, was detected by RT-qPCR, as well as Gata4. After 48h overexpression of miR-208a, GATA4 was detected via western blotting. Dual luciferase reporting system was used to identify the binding sites of miR-208a to Gata4. Results: Expression of miR-208a was upregulated in the CHD group via the control group (p < 0.01). At P7, miR-208a had the highest expression (p < 0.01), and which was highest in myocardiocytes via other organs or tissues (p < 0.01) in adult rats. The number of apoptotic cells increased significantly post-transfection with miR-208a (p < 0.01), while decreased with the miR-208a inhibitor via the control group (p < 0.01). Compared with the control group, there was no significant difference in the expression level of Bcl-2 after miR-208a overexpression (p > 0.05). The present study proved that miR-208a binds directly to the 3´-UTR of Gata4 at site 1,363-1,369 bp. Expression of GATA4 decreased after miR-208a overexpression (p < 0.01), but increased following transfection with a miR-208a inhibitor via the control group (p < 0.05). Conclusions: Our study demonstrated that miR-208a downregulates Bcl-2 by directly targeting GATA4, which may cause CHD. miR-208a may become a new biomarker or therapeutic target for CHD in the future.

Keywords

Simple CHD; miR-208a; GATA4; apoptosis; Bcl-2

Cite This Article

Gong, L., Jiang, H., Qiu, G., Sun, K. (2021). miR-208a Promotes Apoptosis in H9c2 Cardiomyocytes by Targeting GATA4. Congenital Heart Disease, 16(5), 499–512.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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