Advances in CRISPR-based gene editing technology and its application in nucleic acid detection
LIUJIE CHEN1,#, LILI DUAN1,2,#, JIA LI1,2, JUN CHEN1,2, DUANFANG LIAO3, NONGYUE HE4, KAI LI1,3, ZHENG HU1,2,*
BIOCELL, Vol.49, No.1, pp. 21-43, 2025, DOI:10.32604/biocell.2024.056698
- 24 January 2025
Abstract Nucleic acid analysis is a key technique that enables accurate detection of various microorganisms. Conventional nucleic acid testing typically requires access to specialized laboratories, equipment, and trained personnel, which hinders the widespread use of on-site testing for DNA and RNA targets. However, integrating gene editing technology with traditional nucleic acid detection methods, especially isothermal amplification technology, can help overcome the limitations associated with on-site testing. This combination can accomplish precise and swift detection of nucleic acid sequences, offering a robust tool for on-site detection. The Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins (CRISPR/Cas) technology, which More >