Open Access

ARTICLE

DAPK2 promotes autophagy to accelerate the progression of ossification of the posterior longitudinal ligament through the mTORC1 complex

by LEI SHI^1,#, JIANSHI YIN^2,#, YU CHEN¹, JIANGANG SHI¹, JINHAO MIAO^1,*

1 Department of Spine Surgery, Shanghai Changzheng Hospital, Shanghai, 200003, China
2 Department of Orthopedics, The 967th Hospital of Chinese People’s Liberation Army, Dalian, 116000, China

* Corresponding Author: JINHAO MIAO. Email:
# These authors contributed equally to this work

BIOCELL 2024, 48(9), 1389-1400. https://doi.org/10.32604/biocell.2024.049562

Received 10 January 2024; Accepted 19 July 2024; Issue published 04 September 2024

Abstract

Background: Ossification of the posterior longitudinal ligament (OPLL) is a prevalent condition in orthopedics. While death-associated protein kinase 2 (DAPK2) is known to play roles in cellular apoptosis and autophagy, its specific contributions to the advancement of OPLL are not well understood. Methods: Ligament fibroblasts were harvested from patients diagnosed with OPLL. Techniques such as real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) and Western blot analysis were employed to assess DAPK2 levels in both ligament tissues and cultured fibroblasts. The extent of osteogenic differentiation in these cells was evaluated using an alizarin red S (ARS) staining. Additionally, the expression of ossification markers and autophagy markers was quantified. The autophagic activity was further analyzed through LC3 immunofluorescence and transmission electron microscopy (TEM). An in vivo heterotopic bone formation assay was conducted in mice to assess the role of DAPK2 in ossification. Results: Elevated DAPK2 expression was confirmed in both OPLL patient tissues and derived fibroblasts, in contrast to non-OPLL controls. Silencing of DAPK2 significantly curtailed osteogenic differentiation and autophagy in these fibroblasts, evidenced by decreased levels of LC3, and Beclin1, and reduced autophagosome formation. Additionally, DAPK2 was found to inhibit the mechanistic target of the rapamycin complex 1 (mTORC1) complex’s activity. In vivo studies demonstrated that DAPK2 facilitates ossification, and this effect could be counteracted by the mTORC1 inhibitor rapamycin. Conclusion: DAPK2 enhances autophagy and osteogenic processes in OPLL through modulation of the mTORC1 pathway.

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Keywords

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