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Metabolic engineering and genome editing strategies for enhanced lipid production in microalgae

ANJANI DEVI CHINTAGUNTA1, SAMUDRALA PRASHANT JEEVAN KUMAR2, NUNE SATYA SAMPATH KUMAR1,*

1 Department of Biotechnology, Vignan’s Foundation Science, Technology and Research, Vadlamudi, Andhra Pradesh, 522213, India
2 ICAR-Directorate of Floricultural Research, Pune, 412307, India

* Corresponding Author: NUNE SATYA SAMPATH KUMAR. Email: email

BIOCELL 2024, 48(8), 1181-1195. https://doi.org/10.32604/biocell.2024.050540

Abstract

Depleting global petroleum reserves and skyrocketing prices coupled with succinct supply have been a grave concern, which needs alternative sources to conventional fuels. Oleaginous microalgae have been explored for enhanced lipid production, leading towards biodiesel production. These microalgae have short life cycles, require less labor, and space, and are easy to scale up. Triacylglycerol, the primary source of lipids needed to produce biodiesel, is accumulated by most microalgae. The article focuses on different types of oleaginous microalgae, which can be used as a feedstock to produce biodiesel. Lipid biosynthesis in microalgae occurs through fatty acid synthesis and TAG synthesis approaches. In-depth discussions are held regarding other efficient methods for enhancing fatty acid and TAG synthesis, regulating TAG biosynthesis bypass methods, blocking competing pathways, multigene approach, and genome editing. The most potential targets for gene transformation are hypothesized to be a malic enzyme and diacylglycerol acyltransferase while lowering phosphoenolpyruvate carboxylase activity is reported to be advantageous for lipid synthesis.

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APA Style
CHINTAGUNTA, A.D., KUMAR, S.P.J., KUMAR, N.S.S. (2024). Metabolic engineering and genome editing strategies for enhanced lipid production in microalgae. BIOCELL, 48(8), 1181-1195. https://doi.org/10.32604/biocell.2024.050540
Vancouver Style
CHINTAGUNTA AD, KUMAR SPJ, KUMAR NSS. Metabolic engineering and genome editing strategies for enhanced lipid production in microalgae. BIOCELL . 2024;48(8):1181-1195 https://doi.org/10.32604/biocell.2024.050540
IEEE Style
A.D. CHINTAGUNTA, S.P.J. KUMAR, and N.S.S. KUMAR, “Metabolic engineering and genome editing strategies for enhanced lipid production in microalgae,” BIOCELL , vol. 48, no. 8, pp. 1181-1195, 2024. https://doi.org/10.32604/biocell.2024.050540



cc Copyright © 2024 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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