Open Access

REVIEW

Revolutionizing stem cell research: unbiased insights through single-cell sequencing

HAO WU^#, NA HUO^#, SITUO WANG, ZIWEI LIU, YI JIANG^*, QUAN SHI^*

Institute of Stomatology, First Medical Center, Chinese PLA General Hospital, Beijing, 100853, China

* Corresponding Authors: YI JIANG. Email: ; QUAN SHI. Email:
# These authors contributed equally to this work

BIOCELL 2024, 48(11), 1531-1542. https://doi.org/10.32604/biocell.2024.054278

Received 23 May 2024; Accepted 12 July 2024; Issue published 07 November 2024

Abstract

Stem cells have shown great application potential in wound repair, tissue regeneration, and disease treatment. Therefore, a full understanding of stem cells and their related regulatory mechanisms in disease treatment is conducive to improving the therapeutic effect of stem cells. However, thus far, there are still many unsolved mysteries in the field of stem cells due to technical limitations, which hinder the in-depth exploration of stem cells and their wide clinical application. Single-cell sequencing (SCS) has provided very powerful and unbiased insights into cell gene expression profiles at the single-cell level, bringing exciting results to the stem cell field. At present, SCS has been widely applied in the field of stem cells, covering various aspects, including lineage tracing the development of stem cells, identifying new stem cell types, exploring cellular heterogeneity, and identifying internal functional subpopulations. In this paper, we focus on the latest research progress and discuss the application of SCS technology in stem cells.

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Keywords

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