Open Access

ARTICLE

Meiotic nuclear divisions 1 suppresses the proliferation and invasion of pancreatic cancer cells via regulating H2A.X variant histone

by DONGQIN WANG^1,4, YAN SHI¹, ZHIQIANG WANG⁴, JING ZHANG², LUYAO WANG², HONGYU MA³, SHUHUA SHI², XIAOFU LIAN², HUA HUANG⁴, XIAOJING WANG^1,*, CHAOQUN LIAN^4,*

1 Anhui Province Key Laboratory of Clinical and Preclinical Research in Respiratory Disease, Molecular Diagnosis Center, The Department of Pulmonary Critical Care Medicine, First Affiliated Hospital of Bengbu Medical College, Bengbu, 233030, China
2 Department of Genetics, School of Life Sciences, Bengbu Medical College, Bengbu, 233000, China
3 Department of Clinical Medicine, Bengbu Medical College, Bengbu, 233030, China
4 Research Center of Clinical Laboratory Science, Bengbu Medical College, Bengbu, 233030, China

* Corresponding Authors: XIAOJING WANG. Email: ; CHAOQUN LIAN. Email:

BIOCELL 2024, 48(1), 111-122. https://doi.org/10.32604/biocell.2023.046903

Received 18 October 2023; Accepted 23 November 2023; Issue published 30 January 2024

Abstract

Introduction: Among all malignant tumors of the digestive system, pancreatic carcinoma exhibits the highest mortality rate. Currently, prevention and effective treatment are urgent issues that need to be addressed. Methods: The study focused on meiotic nuclear divisions 1 (MND1), integrating data from the Gene Expression Profiling Interactive Analysis (GEPIA) database with prognostic survival analysis. Simultaneously, experiments at cellular level were employed to demonstrate the effect of MND1 on the proliferation and migration of PC. The small-molecule inhibitor of MND1 was used to suppress the migration of PC cells by knocking down MND1 using small interfering RNA (siRNA) in Patu-8988 and Panc1 cell lines. Results: The results of Cell Counting Kit-8 indicated that the suppression of MND1 resulted in a decrease in cell proliferation. Wound healing and Transwell assays revealed that MND1 knockdown reduced cell migration and invasion. Flow cytometry revealed that inhibiting MND1 hindered the cell cycle. Furthermore, MND1 could stimulate the proliferation, migration, and invasion of Patu-8988 and Panc1 cells by increasing the expression of MND1. Notably, MND1 had a positive effect on H2AFX expression in PC cells. Elevated MND1 expression suggests the low overall survival rate of individuals diagnosed with PC. Conclusion: These findings suggest that MND1 has the potential to be a gene with the ability to accurately diagnose and treat PC.

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Keywords

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